PABP1_ARATH
ID PABP1_ARATH Reviewed; 497 AA.
AC F4HT49; Q9FX22;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2013, sequence version 2.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Polyadenylate-binding protein 1;
DE Short=PABP-1;
DE Short=Poly(A)-binding protein 1;
GN Name=PAB1; OrderedLocusNames=At1g34140; ORFNames=F12G12.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=8341686; DOI=10.1073/pnas.90.14.6686;
RA Belostotsky D.A., Meagher R.B.;
RT "Differential organ-specific expression of three poly(A)-binding-protein
RT genes from Arabidopsis thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:6686-6690(1993).
RN [4]
RP GENE FAMILY.
RX PubMed=12586718; DOI=10.1093/genetics/163.1.311;
RA Belostotsky D.A.;
RT "Unexpected complexity of poly(A)-binding protein gene families in
RT flowering plants: three conserved lineages that are at least 200 million
RT years old and possible auto- and cross-regulation.";
RL Genetics 163:311-319(2003).
CC -!- FUNCTION: Binds the poly(A) tail of mRNA. Appears to be an important
CC mediator of the multiple roles of the poly(A) tail in mRNA biogenesis,
CC stability and translation (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in roots but also at lower
CC levels in immature flowers. {ECO:0000269|PubMed:8341686}.
CC -!- MISCELLANEOUS: A.thaliana contains 8 PABP genes.
CC -!- SIMILARITY: Belongs to the polyadenylate-binding protein type-1 family.
CC {ECO:0000305}.
CC -!- CAUTION: Lacks the PABC domain, which is one of the conserved features
CC of the PAPB family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG12523.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AEE31676.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC015446; AAG12523.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31676.1; ALT_SEQ; Genomic_DNA.
DR PIR; E86465; E86465.
DR RefSeq; NP_174676.2; NM_103137.2.
DR AlphaFoldDB; F4HT49; -.
DR SMR; F4HT49; -.
DR BioGRID; 25545; 3.
DR STRING; 3702.AT1G34140.1; -.
DR PaxDb; F4HT49; -.
DR PeptideAtlas; F4HT49; -.
DR PRIDE; F4HT49; -.
DR ProteomicsDB; 248732; -.
DR EnsemblPlants; AT1G34140.1; AT1G34140.1; AT1G34140.
DR GeneID; 840313; -.
DR Gramene; AT1G34140.1; AT1G34140.1; AT1G34140.
DR KEGG; ath:AT1G34140; -.
DR Araport; AT1G34140; -.
DR TAIR; locus:2009036; AT1G34140.
DR eggNOG; KOG0118; Eukaryota.
DR HOGENOM; CLU_012062_22_7_1; -.
DR OrthoDB; 1027234at2759; -.
DR PRO; PR:F4HT49; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HT49; baseline and differential.
DR Genevisible; F4HT49; AT.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IBA:GO_Central.
DR GO; GO:0008143; F:poly(A) binding; IBA:GO_Central.
DR GO; GO:0008266; F:poly(U) RNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR CDD; cd12379; RRM2_I_PABPs; 1.
DR Gene3D; 3.30.70.330; -; 4.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR006515; PABP_1234.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR045305; RRM2_I_PABPs.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR003954; RRM_dom_euk.
DR Pfam; PF00076; RRM_1; 4.
DR SMART; SM00360; RRM; 4.
DR SMART; SM00361; RRM_1; 2.
DR SUPFAM; SSF54928; SSF54928; 2.
DR TIGRFAMs; TIGR01628; PABP-1234; 1.
DR PROSITE; PS50102; RRM; 4.
PE 2: Evidence at transcript level;
KW Cytoplasm; Nucleus; Reference proteome; Repeat; RNA-binding;
KW Translation regulation.
FT CHAIN 1..497
FT /note="Polyadenylate-binding protein 1"
FT /id="PRO_0000422642"
FT DOMAIN 32..111
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 121..198
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 209..286
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 313..390
FT /note="RRM 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
SQ SEQUENCE 497 AA; 56214 MW; EE22117397BCF9A2 CRC64;
MTLEPDIYLK KIIREQRRVS TIRTSENKFE KHTLYIGDLD SSVEEGDLYT KFSEFTHDIS
SVKVCCDRNT GLSLGYGYVN FNHSSQAKTA MEVLNYCKLK GKPMRIMFSE RDPSNRMSGR
GNVFVKNLDE SIDNKQLCDM FSAFGKVLSC KVARDASGVS KGYGFVQFYS DLSVYTACNF
HNGTLIRNQH IHVCPFVSRG QWDKSRVFTN VYVKNLVETA TDADLKRLFG EFGEITSAVV
MKDGEGKSRR FGFVNFEKAE AAVTAIEKMN GVVVDEKELH VGRAQRKTNR TEDLKAKFEL
EKIIRDMKTR KGMNLYVKNL DDSVDNTKLE ELFSEFGTIT SCKVMVHSNG ISKGVGFVEF
STSEEASKAM LKMNGKMVGN KPIYVSLAQC KEQHKLHLQT QFNNPPPSPH QQPIFSQVVA
PATMLSQQTP LRGYNFQPYS MCGSRMPNSC PPISIPNFMV PQPFRPTLYP PAPLVGLYGS
LPQPLLQPNL WNPYRRF
