ASK14_ARATH
ID ASK14_ARATH Reviewed; 149 AA.
AC O81057;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=SKP1-like protein 14;
DE Short=AtSK14;
GN Name=ASK14; OrderedLocusNames=At2g03170; ORFNames=T18E12.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=12970487; DOI=10.1104/pp.103.024703;
RA Zhao D., Ni W., Feng B., Han T., Petrasek M.G., Ma H.;
RT "Members of the Arabidopsis-SKP1-like gene family exhibit a variety of
RT expression patterns and may play diverse roles in Arabidopsis.";
RL Plant Physiol. 133:203-217(2003).
RN [5]
RP TISSUE SPECIFICITY, AND INTERACTION WITH AT4G39550; AT3G61590 AND
RP AT5G49610.
RX PubMed=14749489; DOI=10.1093/pcp/pch009;
RA Takahashi N., Kuroda H., Kuromori T., Hirayama T., Seki M., Shinozaki K.,
RA Shimada H., Matsui M.;
RT "Expression and interaction analysis of Arabidopsis Skp1-related genes.";
RL Plant Cell Physiol. 45:83-91(2004).
RN [6]
RP INTERACTION WITH CPR1/CPR30.
RX PubMed=19682297; DOI=10.1111/j.1365-313x.2009.03995.x;
RA Gou M., Su N., Zheng J., Huai J., Wu G., Zhao J., He J., Tang D., Yang S.,
RA Wang G.;
RT "An F-box gene, CPR30, functions as a negative regulator of the defense
RT response in Arabidopsis.";
RL Plant J. 60:757-770(2009).
CC -!- FUNCTION: Involved in ubiquitination and subsequent proteasomal
CC degradation of target proteins. Together with CUL1, RBX1 and a F-box
CC protein, it forms a SCF E3 ubiquitin ligase complex. The functional
CC specificity of this complex depends on the type of F-box protein. In
CC the SCF complex, it serves as an adapter that links the F-box protein
CC to CUL1 (By similarity). {ECO:0000250, ECO:0000269|PubMed:12970487}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By
CC similarity). Interacts with CPR1/CPR30, At3g61590, At4g39550 and
CC At5g49610. {ECO:0000250, ECO:0000269|PubMed:14749489,
CC ECO:0000269|PubMed:19682297}.
CC -!- INTERACTION:
CC O81057; Q8LEA8: EID1; NbExp=3; IntAct=EBI-401221, EBI-687388;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Restricted to inflorescences, pollen and leaves.
CC {ECO:0000269|PubMed:12970487, ECO:0000269|PubMed:14749489}.
CC -!- DEVELOPMENTAL STAGE: Expressed in male meiocytes and tetrads.
CC {ECO:0000269|PubMed:12970487}.
CC -!- SIMILARITY: Belongs to the SKP1 family. {ECO:0000305}.
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DR EMBL; AC005313; AAC34485.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05671.1; -; Genomic_DNA.
DR EMBL; DQ056526; AAY78682.1; -; mRNA.
DR PIR; T02709; T02709.
DR RefSeq; NP_565296.1; NM_126368.2.
DR AlphaFoldDB; O81057; -.
DR SMR; O81057; -.
DR BioGRID; 248; 70.
DR ComplexPortal; CPX-1441; SCF(COI1) ubiquitin ligase complex, variant CUL1-RBX1A-ASK14.
DR ComplexPortal; CPX-1462; SCF(COI1) ubiquitin ligase complex, variant CUL1-RBX1B-ASK14.
DR ComplexPortal; CPX-1484; SCF(COI1) ubiquitin ligase complex, variant CUL2-RBX1A-ASK14.
DR ComplexPortal; CPX-1507; SCF(COI1) ubiquitin ligase complex, variant CUL2-RBX1B-ASK14.
DR ComplexPortal; CPX-1527; SCF(TIR1) ubiquitin ligase complex, variant CUL1-RBX1A-ASK14.
DR ComplexPortal; CPX-1548; SCF(TIR1) ubiquitin ligase complex, variant CUL1-RBX1B-ASK14.
DR ComplexPortal; CPX-1570; SCF(TIR1) ubiquitin ligase complex, variant CUL2-RBX1A-ASK14.
DR ComplexPortal; CPX-1591; SCF(TIR1) ubiquitin ligase complex, variant CUL2-RBX1B-ASK14.
DR DIP; DIP-31332N; -.
DR IntAct; O81057; 19.
DR STRING; 3702.AT2G03170.1; -.
DR PaxDb; O81057; -.
DR PRIDE; O81057; -.
DR ProteomicsDB; 246505; -.
DR EnsemblPlants; AT2G03170.1; AT2G03170.1; AT2G03170.
DR GeneID; 814846; -.
DR Gramene; AT2G03170.1; AT2G03170.1; AT2G03170.
DR KEGG; ath:AT2G03170; -.
DR Araport; AT2G03170; -.
DR TAIR; locus:2056956; AT2G03170.
DR eggNOG; KOG1724; Eukaryota.
DR HOGENOM; CLU_059252_6_1_1; -.
DR InParanoid; O81057; -.
DR OMA; AREFQVI; -.
DR OrthoDB; 1412723at2759; -.
DR PhylomeDB; O81057; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:O81057; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O81057; baseline and differential.
DR Genevisible; O81057; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IC:ComplexPortal.
DR GO; GO:0097602; F:cullin family protein binding; IBA:GO_Central.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IC:ComplexPortal.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IMP:ComplexPortal.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0009733; P:response to auxin; IC:ComplexPortal.
DR GO; GO:0009753; P:response to jasmonic acid; IMP:ComplexPortal.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; TAS:TAIR.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR016897; SKP1.
DR InterPro; IPR001232; SKP1-like.
DR InterPro; IPR036296; SKP1-like_dim_sf.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR InterPro; IPR016072; Skp1_comp_dimer.
DR InterPro; IPR016073; Skp1_comp_POZ.
DR PANTHER; PTHR11165; PTHR11165; 1.
DR Pfam; PF01466; Skp1; 1.
DR Pfam; PF03931; Skp1_POZ; 1.
DR PIRSF; PIRSF028729; E3_ubiquit_lig_SCF_Skp; 1.
DR SMART; SM00512; Skp1; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR SUPFAM; SSF81382; SSF81382; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..149
FT /note="SKP1-like protein 14"
FT /id="PRO_0000375255"
FT REGION 91..149
FT /note="Interaction with the F-box domain of F-box proteins"
FT /evidence="ECO:0000250"
SQ SEQUENCE 149 AA; 17102 MW; 276BB2CB0AF5DE01 CRC64;
MSSNKIVLSS SDGESFEVEE AVARKLKIVE HMIEDDCVVT EVPLQNVTGK ILSIVVEYCK
KHVVDEESDE FKTWDEEFMK KFDQPTVFQL LLAANYLNIK GLLDLSAQTV ADRIKDKTPE
EIREIFNIEN DFTPEEEAAV RKENAWAFE
