ASK15_ARATH
ID ASK15_ARATH Reviewed; 177 AA.
AC Q1PEL7; Q9LI78;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=SKP1-like protein 15;
DE Short=AtSK15;
GN Name=ASK15; OrderedLocusNames=At3g25650; ORFNames=T5M7.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=12970487; DOI=10.1104/pp.103.024703;
RA Zhao D., Ni W., Feng B., Han T., Petrasek M.G., Ma H.;
RT "Members of the Arabidopsis-SKP1-like gene family exhibit a variety of
RT expression patterns and may play diverse roles in Arabidopsis.";
RL Plant Physiol. 133:203-217(2003).
CC -!- FUNCTION: Involved in ubiquitination and subsequent proteasomal
CC degradation of target proteins. Together with CUL1, RBX1 and a F-box
CC protein, it forms a SCF E3 ubiquitin ligase complex. The functional
CC specificity of this complex depends on the type of F-box protein. In
CC the SCF complex, it serves as an adapter that links the F-box protein
CC to CUL1 (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q1PEL7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q1PEL7-2; Sequence=VSP_037362, VSP_037363;
CC -!- TISSUE SPECIFICITY: Expressed at low levels in seedlings and leaves.
CC {ECO:0000269|PubMed:12970487}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in the pith and vascular bundle
CC in the stem. Found in the pedicel of young buds. Also expressed in the
CC inner epidermis of carpels and pedicels in mature flowers. In siliques,
CC mostly expressed in inner epidermis of the valve.
CC {ECO:0000269|PubMed:12970487}.
CC -!- SIMILARITY: Belongs to the SKP1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB03085.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP001313; BAB03085.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE77046.1; -; Genomic_DNA.
DR EMBL; DQ446700; ABE65968.1; -; mRNA.
DR RefSeq; NP_566773.1; NM_113463.1. [Q1PEL7-1]
DR AlphaFoldDB; Q1PEL7; -.
DR SMR; Q1PEL7; -.
DR BioGRID; 7483; 3.
DR ComplexPortal; CPX-1442; SCF(COI1) ubiquitin ligase complex, variant CUL1-RBX1A-ASK15.
DR ComplexPortal; CPX-1463; SCF(COI1) ubiquitin ligase complex, variant CUL1-RBX1B-ASK15.
DR ComplexPortal; CPX-1485; SCF(COI1) ubiquitin ligase complex, variant CUL2-RBX1A-ASK15.
DR ComplexPortal; CPX-1508; SCF(COI1) ubiquitin ligase complex, variant CUL2-RBX1B-ASK15.
DR ComplexPortal; CPX-1528; SCF(TIR1) ubiquitin ligase complex, variant CUL1-RBX1A-ASK15.
DR ComplexPortal; CPX-1549; SCF(TIR1) ubiquitin ligase complex, variant CUL1-RBX1B-ASK15.
DR ComplexPortal; CPX-1571; SCF(TIR1) ubiquitin ligase complex, variant CUL2-RBX1A-ASK15.
DR ComplexPortal; CPX-1592; SCF(TIR1) ubiquitin ligase complex, variant CUL2-RBX1B-ASK15.
DR IntAct; Q1PEL7; 5.
DR STRING; 3702.AT3G25650.1; -.
DR PaxDb; Q1PEL7; -.
DR PRIDE; Q1PEL7; -.
DR EnsemblPlants; AT3G25650.1; AT3G25650.1; AT3G25650. [Q1PEL7-1]
DR GeneID; 822152; -.
DR Gramene; AT3G25650.1; AT3G25650.1; AT3G25650. [Q1PEL7-1]
DR KEGG; ath:AT3G25650; -.
DR Araport; AT3G25650; -.
DR TAIR; locus:2102370; AT3G25650.
DR eggNOG; KOG1724; Eukaryota.
DR HOGENOM; CLU_059252_6_1_1; -.
DR InParanoid; Q1PEL7; -.
DR OMA; NYLNCKG; -.
DR OrthoDB; 1412723at2759; -.
DR PhylomeDB; Q1PEL7; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q1PEL7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q1PEL7; baseline and differential.
DR Genevisible; Q1PEL7; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; ISS:TAIR.
DR GO; GO:0097602; F:cullin family protein binding; IBA:GO_Central.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IC:ComplexPortal.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IMP:ComplexPortal.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0009733; P:response to auxin; IC:ComplexPortal.
DR GO; GO:0009753; P:response to jasmonic acid; IMP:ComplexPortal.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR016897; SKP1.
DR InterPro; IPR001232; SKP1-like.
DR InterPro; IPR036296; SKP1-like_dim_sf.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR InterPro; IPR016072; Skp1_comp_dimer.
DR InterPro; IPR016073; Skp1_comp_POZ.
DR PANTHER; PTHR11165; PTHR11165; 1.
DR Pfam; PF01466; Skp1; 1.
DR Pfam; PF03931; Skp1_POZ; 1.
DR PIRSF; PIRSF028729; E3_ubiquit_lig_SCF_Skp; 1.
DR SMART; SM00512; Skp1; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR SUPFAM; SSF81382; SSF81382; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Nucleus; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..177
FT /note="SKP1-like protein 15"
FT /id="PRO_0000375256"
FT REGION 108..167
FT /note="Interaction with the F-box domain of F-box proteins"
FT /evidence="ECO:0000250"
FT VAR_SEQ 83..112
FT /note="EAKQNLDAWDAEFMKNIDMETIFKLILAAN -> VAGRFLNQLKKEMMHLRR
FT RSRISMLGTQSS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17147637"
FT /id="VSP_037362"
FT VAR_SEQ 113..177
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17147637"
FT /id="VSP_037363"
SQ SEQUENCE 177 AA; 20254 MW; 05C92321D745D219 CRC64;
MSSNKIVLTS SDGESFQVEE VVARKLQIVK HLLEDDCVIN EIPLQNVTGN ILSIVLEYCK
KHVDDVVDDD ASEEPKKKKP DDEAKQNLDA WDAEFMKNID METIFKLILA ANYLNVEGLL
GLTCQTVADY IKDKTPEEVR ELFNIENDFT HEEEEEAIRK ENAWAFEADT KHEDPKP
