ID   PAC2_PSES3              Reviewed;         774 AA.
AC   P15558;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Penicillin acylase 2 proenzyme;
DE            EC=3.5.1.11;
DE   AltName: Full=Cephalosporin acylase II;
DE   AltName: Full=Penicillin acylase II;
DE   AltName: Full=Penicillin amidase II;
DE   Contains:
DE     RecName: Full=Penicillin acylase 2 small subunit;
DE     AltName: Full=Penicillin acylase II small subunit;
DE   Contains:
DE     RecName: Full=Penicillin acylase 2 large subunit;
DE     AltName: Full=Penicillin acylase II large subunit;
DE   Flags: Precursor;
GN   Name=acyII;
OS   Pseudomonas sp. (strain SE83).
OC   Bacteria; Proteobacteria.
OX   NCBI_TaxID=309;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-38 AND 240-276,
RP   AND SUBUNIT.
RC   STRAIN=SE83;
RX   PubMed=3680178; DOI=10.1128/jb.169.12.5821-5826.1987;
RA   Matsuda A., Toma K., Komatsu K.;
RT   "Nucleotide sequences of the genes for two distinct cephalosporin acylases
RT   from a Pseudomonas strain.";
RL   J. Bacteriol. 169:5821-5826(1987).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + penicillin = 6-aminopenicillanate + a carboxylate;
CC         Xref=Rhea:RHEA:18693, ChEBI:CHEBI:15377, ChEBI:CHEBI:29067,
CC         ChEBI:CHEBI:51356, ChEBI:CHEBI:57869; EC=3.5.1.11;
CC   -!- SUBUNIT: Heterodimer of a small subunit and a large subunit processed
CC       from the same precursor. {ECO:0000269|PubMed:3680178}.
CC   -!- SIMILARITY: Belongs to the peptidase S45 family. {ECO:0000305}.
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DR   EMBL; M18278; AAA25690.1; -; Genomic_DNA.
DR   PIR; A28392; A28392.
DR   AlphaFoldDB; P15558; -.
DR   SMR; P15558; -.
DR   MEROPS; S45.003; -.
DR   PRIDE; P15558; -.
DR   BRENDA; 3.5.1.93; 5085.
DR   GO; GO:0008953; F:penicillin amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1400.10; -; 1.
DR   Gene3D; 1.10.439.10; -; 1.
DR   Gene3D; 2.30.120.10; -; 1.
DR   Gene3D; 3.60.20.10; -; 1.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR   InterPro; IPR043147; Penicillin_amidase_A-knob.
DR   InterPro; IPR023343; Penicillin_amidase_dom1.
DR   InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR   InterPro; IPR002692; S45.
DR   PANTHER; PTHR34218; PTHR34218; 1.
DR   Pfam; PF01804; Penicil_amidase; 1.
DR   PIRSF; PIRSF001227; Pen_acylase; 1.
DR   SUPFAM; SSF56235; SSF56235; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Direct protein sequencing; Hydrolase; Zymogen.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:3680178"
FT   CHAIN           2..774
FT                   /note="Penicillin acylase 2 proenzyme"
FT                   /id="PRO_0000253349"
FT   CHAIN           2..?239
FT                   /note="Penicillin acylase 2 small subunit"
FT                   /id="PRO_0000021991"
FT   CHAIN           240..774
FT                   /note="Penicillin acylase 2 large subunit"
FT                   /id="PRO_0000021992"
FT   ACT_SITE        240
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   774 AA;  83694 MW;  1AE1A6EE53010B94 CRC64;
     MTMAAKTDRE ALQAALPPLS GSLSIPGLSA PVRVQRDGWG IPHIKASGEA DAYRALGFVH
     AQDRLFQMEL TRRKALGRAA EWLGAEAAEA DILVRRLGME KVCRRDFEAL GAEAKDMLRA
     YVAGVNAFLA SGAPLPIEYG LLGAEPEPWE PWHSIAVMRR LGLLMGSVWF KLWRMLALPV
     VGAANALKLR YDDGGQDLLC IPPGVEAERL EADLAALRPA VDALLKAMGG DASDAAGGGS
     NNWAVAPGRT ATGRPILAGD PHRVFEIPGM YAQHHLACDR FDMIGLTVPG VPGFPHFAHN
     GKVAYCVTHA FMDIHDLYLE QFAEDGRTAR FGNEFEPVAW RRDRIAVRGG ADREFDIVET
     RHGPVIAGDP LEGAALTLRS VQFAETDLSF DCLTRMPGAS TVAQLYDATR GWGLIDHNLV
     AGDVAGSIGH LVRARVPSRP RENGWLPVPG WSGEHEWRGW IPHEAMPRVI DPPGGLIVTA
     NNRVVADDHP DYLCTDCHPP YRAERIMERL VASPAFAVDD AAAIHADTLS PHVGLLRARL
     EALGIQGSLP AEELRQTLIA WDGRMDAGSQ AASAYNAFRR ALTRLVTARS GLEQAIAHPF
     AAVPPGVSPQ GQVWWAVPTL LRNDDAGMLK GWSWDEALSE ALSVATQNLT GRGWGEEHRP
     RFTHPLSAQF PAWAALLNPV SRPIGGDGDT VLANGLVPSA GPEATYGALS RYVFDVGNWD
     NSRWVVFHGA SGHPASPHYA DQNAPWSDCA MVPMLYSWDR IAAEAVTSQE LVPA