PAC2_YEAST
ID PAC2_YEAST Reviewed; 518 AA.
AC P39937; D3DLQ3;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Protein PAC2;
GN Name=PAC2; OrderedLocusNames=YER007W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9215891; DOI=10.1093/genetics/146.3.849;
RA Hoyt M.A., Macke J.P., Roberts B.T., Geiser J.R.;
RT "Saccharomyces cerevisiae PAC2 functions with CIN1, 2 and 4 in a pathway
RT leading to normal microtubule stability.";
RL Genetics 146:849-857(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for viability in the absence of the kinesin-related
CC CIN8 mitotic motor. Seems to be involved in the assembly of alpha-
CC tubulin.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 2140 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U16814; AAB00683.1; -; Genomic_DNA.
DR EMBL; U18778; AAB64540.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07657.1; -; Genomic_DNA.
DR PIR; S50465; S50465.
DR RefSeq; NP_010922.1; NM_001178898.1.
DR AlphaFoldDB; P39937; -.
DR SMR; P39937; -.
DR BioGRID; 36737; 108.
DR DIP; DIP-2342N; -.
DR IntAct; P39937; 2.
DR MINT; P39937; -.
DR STRING; 4932.YER007W; -.
DR MaxQB; P39937; -.
DR PaxDb; P39937; -.
DR PRIDE; P39937; -.
DR EnsemblFungi; YER007W_mRNA; YER007W; YER007W.
DR GeneID; 856724; -.
DR KEGG; sce:YER007W; -.
DR SGD; S000000809; PAC2.
DR VEuPathDB; FungiDB:YER007W; -.
DR eggNOG; KOG3207; Eukaryota.
DR GeneTree; ENSGT00530000063405; -.
DR HOGENOM; CLU_017716_5_1_1; -.
DR InParanoid; P39937; -.
DR OMA; KKYALDW; -.
DR BioCyc; YEAST:G3O-30194-MON; -.
DR PRO; PR:P39937; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P39937; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0043014; F:alpha-tubulin binding; IPI:SGD.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0007023; P:post-chaperonin tubulin folding pathway; IBA:GO_Central.
DR GO; GO:0006457; P:protein folding; IGI:SGD.
DR GO; GO:0007021; P:tubulin complex assembly; IMP:SGD.
DR Gene3D; 2.30.30.190; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR036859; CAP-Gly_dom_sf.
DR InterPro; IPR000938; CAP-Gly_domain.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR044994; TBCE.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR15140; PTHR15140; 1.
DR Pfam; PF01302; CAP_GLY; 1.
DR Pfam; PF13516; LRR_6; 2.
DR SMART; SM01052; CAP_GLY; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR SUPFAM; SSF74924; SSF74924; 1.
DR PROSITE; PS00845; CAP_GLY_1; 1.
DR PROSITE; PS50245; CAP_GLY_2; 1.
PE 1: Evidence at protein level;
KW Chaperone; Cytoplasm; Cytoskeleton; Leucine-rich repeat; Microtubule;
KW Reference proteome; Repeat.
FT CHAIN 1..518
FT /note="Protein PAC2"
FT /id="PRO_0000083526"
FT DOMAIN 23..67
FT /note="CAP-Gly"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00045"
FT REPEAT 153..174
FT /note="LRR 1"
FT REPEAT 179..201
FT /note="LRR 2"
FT REPEAT 204..227
FT /note="LRR 3"
FT REPEAT 229..252
FT /note="LRR 4"
FT REPEAT 255..276
FT /note="LRR 5"
FT REPEAT 277..298
FT /note="LRR 6"
FT REPEAT 299..319
FT /note="LRR 7"
FT REPEAT 324..345
FT /note="LRR 8"
SQ SEQUENCE 518 AA; 59258 MW; 3997C60547D04A4A CRC64;
MTYEIGDRLK IGGYFCTIKF IGVIKPWPSV KAYGVEWDDH SRGKHSGTID DIHYFDVQIP
NSGSFLKESK IKSPGVRRIT FYEALSEKYG GSSNNINDLS IGNKRVEGLG FDELNARNKN
YKKLRKIALR DSDVAILFQN QDELNRVIQN CVNVKDLDLS LNLFTNINSL CEFIEPLKNL
ESLNISQNKL LSGWDNLKEY DLSHIKTLRL SSCGLSYKHI GKLLKSFRTL KMLDLSYNNL
TSAGIQNFEN EIPCTLEELN ISGNNLISFP LFPKNLTLKG LNVSNNQISR APSIAIYSVE
SLDITDNKFK ERSLIDDLNK TFPSLKNIHL SGNEFNYNGN YINVEEQATF YEVLARFDRV
MVLNGSICDV KTRREAEMFF VSKVMNNELS YDTNLPRWSS LIKSYEIDMS KLSFNNERET
RQSLVLKIKI RAGKKPSSDL DYWVLPSFTV RYVKSVICRK LNFDILNVKL FHENSEGMIN
EIKYNFRPIS DFNVVNGDII HVSSPVNNKS IQKVNSPS
