ID   PACA_CLAMA              Reviewed;         195 AA.
AC   P48144;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Glucagon family neuropeptides;
DE   Contains:
DE     RecName: Full=Growth hormone-releasing factor;
DE              Short=GRF;
DE     AltName: Full=Growth hormone-releasing hormone;
DE              Short=GHRH;
DE   Contains:
DE     RecName: Full=Pituitary adenylate cyclase-activating polypeptide;
DE              Short=PACAP;
DE   Flags: Precursor;
OS   Clarias macrocephalus (Bighead catfish).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Siluriformes;
OC   Clariidae; Clarias.
OX   NCBI_TaxID=35657;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   PubMed=7758831; DOI=10.1016/0303-7207(94)03467-8;
RA   McRory J.E., Parker D.B., Ngamvongchon S., Sherwood N.M.;
RT   "Sequence and expression of cDNA for pituitary adenylate cyclase activating
RT   polypeptide (PACAP) and growth hormone-releasing hormone (GHRH)-like
RT   peptide in catfish.";
RL   Mol. Cell. Endocrinol. 108:169-177(1995).
CC   -!- FUNCTION: Primary role of GHRH is to release GH from the pituitary.
CC   -!- FUNCTION: PACAP plays pivotal roles as a neurotransmitter and/or a
CC       neuromodulator.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Brain, testis, ovary and stomach. Not pancreas,
CC       pituitary, muscle and liver.
CC   -!- SIMILARITY: Belongs to the glucagon family. {ECO:0000305}.
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DR   EMBL; X79078; CAA55684.1; -; mRNA.
DR   PIR; I50456; I50456.
DR   AlphaFoldDB; P48144; -.
DR   SMR; P48144; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   InterPro; IPR015550; Glucagon.
DR   InterPro; IPR000532; Glucagon_GIP_secretin_VIP.
DR   PANTHER; PTHR11213; PTHR11213; 1.
DR   Pfam; PF00123; Hormone_2; 2.
DR   PRINTS; PR00275; GLUCAGON.
DR   SMART; SM00070; GLUCA; 2.
DR   PROSITE; PS00260; GLUCAGON; 1.
PE   2: Evidence at transcript level;
KW   Amidation; Cleavage on pair of basic residues; Hormone; Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..82
FT                   /id="PRO_0000011521"
FT   PEPTIDE         83..127
FT                   /note="Growth hormone-releasing factor"
FT                   /id="PRO_0000011522"
FT   PEPTIDE         130..167
FT                   /note="Pituitary adenylate cyclase-activating polypeptide"
FT                   /id="PRO_0000011523"
FT   PROPEP          171..195
FT                   /id="PRO_0000011524"
FT   REGION          113..132
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         167
FT                   /note="Lysine amide"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   195 AA;  22443 MW;  DD34811FECAA5B51 CRC64;
     MAKSSRATLA LLIYGILMRY SQCTPIGMGF PNMRLDNDVF GDEGNSLSEL SYEPDTMSAR
     SRPALPEDAY TLYYPPERRA ETHADGLLDR ALRDILVQLS ARKYLHSLTA VRVGEEEEDE
     EDSEPLSKRH SDGIFTDSYS RYRKQMAVKK YLAAVLGRRY RQRFRNKGRR LVVPSVWTGI
     RDTVIITPEK RGKRY