ID   ASK1_ASHGO              Reviewed;         181 AA.
AC   Q75DE8;
DT   21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=DASH complex subunit ASK1;
DE   AltName: Full=Associated with spindles and kinetochores protein 1;
DE   AltName: Full=Outer kinetochore protein ASK1;
GN   Name=ASK1; OrderedLocusNames=ABR079C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Component of the DASH complex, a microtubule-binding
CC       subcomplex of the outer kinetochore that is essential for proper
CC       chromosome segregation. The DASH complex mediates the formation and
CC       maintenance of bipolar kinetochore-microtubule attachments by forming
CC       closed rings around spindle microtubules and establishing interactions
CC       with proteins from the central kinetochore (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: The DASH complex oligomerizes to form rings that encircle the
CC       microtubules. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm, cytoskeleton,
CC       spindle {ECO:0000250}. Chromosome, centromere, kinetochore
CC       {ECO:0000250}. Note=Associates with the mitotic spindle and the
CC       kinetochore. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DASH complex ASK1 family. {ECO:0000305}.
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DR   EMBL; AE016815; AAS50849.1; -; Genomic_DNA.
DR   RefSeq; NP_983025.1; NM_208378.1.
DR   AlphaFoldDB; Q75DE8; -.
DR   SMR; Q75DE8; -.
DR   STRING; 33169.AAS50849; -.
DR   EnsemblFungi; AAS50849; AAS50849; AGOS_ABR079C.
DR   GeneID; 4619129; -.
DR   KEGG; ago:AGOS_ABR079C; -.
DR   eggNOG; ENOG502S2V2; Eukaryota.
DR   HOGENOM; CLU_090087_0_0_1; -.
DR   InParanoid; Q75DE8; -.
DR   OMA; TIIHFST; -.
DR   Proteomes; UP000000591; Chromosome II.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0042729; C:DASH complex; IBA:GO_Central.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0072686; C:mitotic spindle; IEA:InterPro.
DR   GO; GO:0044732; C:mitotic spindle pole body; IBA:GO_Central.
DR   GO; GO:0051010; F:microtubule plus-end binding; IEA:EnsemblFungi.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:1990758; P:mitotic sister chromatid biorientation; IEA:EnsemblFungi.
DR   GO; GO:0051987; P:positive regulation of attachment of spindle microtubules to kinetochore; IEA:EnsemblFungi.
DR   GO; GO:0031116; P:positive regulation of microtubule polymerization; IEA:EnsemblFungi.
DR   InterPro; IPR013964; DASH_Ask1.
DR   PANTHER; PTHR28200; PTHR28200; 1.
DR   Pfam; PF08655; DASH_Ask1; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW   Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..181
FT                   /note="DASH complex subunit ASK1"
FT                   /id="PRO_0000211313"
FT   REGION          77..181
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        135..159
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   181 AA;  19596 MW;  874FD9D56779F627 CRC64;
     MARQPPAPEP TLEQLDQEIT LQLQKIDANL SACFSKITKD IIPAVARYGA VCDDALSACS
     WLRELFQKSS DIQLATEPAA PAPAAESLFP QAPAPRAPEV TTEGHVLAVP VSSDDEPDDG
     STLQRQHKRR KLSLQLQQRY GSSSSSSFVS RSPTLRTRPA SSPLRAPDDP DPPAVLRFAA
     R