PACA_MOUSE
ID PACA_MOUSE Reviewed; 175 AA.
AC O70176;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Pituitary adenylate cyclase-activating polypeptide;
DE Short=PACAP;
DE Contains:
DE RecName: Full=PACAP-related peptide;
DE AltName: Full=PRP-48;
DE Contains:
DE RecName: Full=Pituitary adenylate cyclase-activating polypeptide 27;
DE Short=PACAP-27;
DE Short=PACAP27;
DE Contains:
DE RecName: Full=Pituitary adenylate cyclase-activating polypeptide 38;
DE Short=PACAP-38;
DE Short=PACAP38;
DE Flags: Precursor;
GN Name=Adcyap1; Synonyms=Pacap;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/SvJ;
RX PubMed=9573339; DOI=10.1016/s0378-1119(98)00110-3;
RA Yamamoto K., Hashimoto H., Hagihara N., Nishino A., Fujita T., Matsuda T.,
RA Baba A.;
RT "Cloning and characterization of the mouse pituitary adenylate cyclase-
RT activating polypeptide (PACAP) gene.";
RL Gene 211:63-69(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION.
RX PubMed=23913443; DOI=10.1210/en.2013-1167;
RA Prevost G., Arabo A., Jian L., Quelennec E., Cartier D., Hassan S.,
RA Falluel-Morel A., Tanguy Y., Gargani S., Lihrmann I., Kerr-Conte J.,
RA Lefebvre H., Pattou F., Anouar Y.;
RT "The PACAP-regulated gene selenoprotein T is abundantly expressed in mouse
RT and human beta-cells and its targeted inactivation impairs glucose
RT tolerance.";
RL Endocrinology 154:3796-3806(2013).
CC -!- FUNCTION: Binding to its receptor activates G proteins and stimulates
CC adenylate cyclase in pituitary cells (By similarity). Promotes neuron
CC projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway (By
CC similarity). In chromaffin cells, induces long-lasting increase of
CC intracellular calcium concentrations and neuroendocrine secretion (By
CC similarity). Involved in the control of glucose homeostasis, induces
CC insulin secretion by pancreatic beta cells (PubMed:23913443).
CC {ECO:0000250|UniProtKB:P13589, ECO:0000250|UniProtKB:P18509,
CC ECO:0000250|UniProtKB:Q29W19, ECO:0000269|PubMed:23913443}.
CC -!- SUBUNIT: Interacts with ADCYAP1R1 (via N-terminal extracellular
CC domain). {ECO:0000250|UniProtKB:P18509}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the glucagon family. {ECO:0000305}.
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DR EMBL; AB010149; BAA28355.1; -; Genomic_DNA.
DR EMBL; BC057344; AAH57344.1; -; mRNA.
DR CCDS; CCDS29029.1; -.
DR RefSeq; NP_001302432.1; NM_001315503.1.
DR RefSeq; NP_001302433.1; NM_001315504.1.
DR RefSeq; NP_033755.1; NM_009625.3.
DR AlphaFoldDB; O70176; -.
DR BMRB; O70176; -.
DR SMR; O70176; -.
DR BioGRID; 197979; 2.
DR STRING; 10090.ENSMUSP00000067057; -.
DR PhosphoSitePlus; O70176; -.
DR PaxDb; O70176; -.
DR PRIDE; O70176; -.
DR ProteomicsDB; 294323; -.
DR Antibodypedia; 41707; 287 antibodies from 31 providers.
DR DNASU; 11516; -.
DR Ensembl; ENSMUST00000064775; ENSMUSP00000067057; ENSMUSG00000024256.
DR Ensembl; ENSMUST00000234081; ENSMUSP00000157279; ENSMUSG00000024256.
DR GeneID; 11516; -.
DR KEGG; mmu:11516; -.
DR UCSC; uc008dwi.1; mouse.
DR CTD; 116; -.
DR MGI; MGI:105094; Adcyap1.
DR VEuPathDB; HostDB:ENSMUSG00000024256; -.
DR eggNOG; ENOG502QSGB; Eukaryota.
DR GeneTree; ENSGT00950000183154; -.
DR HOGENOM; CLU_118633_0_0_1; -.
DR InParanoid; O70176; -.
DR OMA; QDFAYDQ; -.
DR OrthoDB; 1415722at2759; -.
DR PhylomeDB; O70176; -.
DR TreeFam; TF332804; -.
DR Reactome; R-MMU-418555; G alpha (s) signalling events.
DR Reactome; R-MMU-420092; Glucagon-type ligand receptors.
DR BioGRID-ORCS; 11516; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Mzb1; mouse.
DR PRO; PR:O70176; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; O70176; protein.
DR Bgee; ENSMUSG00000024256; Expressed in basal plate medulla oblongata and 108 other tissues.
DR ExpressionAtlas; O70176; baseline and differential.
DR Genevisible; O70176; MM.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0043204; C:perikaryon; IBA:GO_Central.
DR GO; GO:0043195; C:terminal bouton; ISO:MGI.
DR GO; GO:0005184; F:neuropeptide hormone activity; ISS:UniProtKB.
DR GO; GO:0051428; F:peptide hormone receptor binding; ISS:UniProtKB.
DR GO; GO:0016521; F:pituitary adenylate cyclase activating polypeptide activity; IDA:BHF-UCL.
DR GO; GO:0031858; F:pituitary adenylate cyclase-activating polypeptide receptor binding; ISO:MGI.
DR GO; GO:0005102; F:signaling receptor binding; ISO:MGI.
DR GO; GO:0007190; P:activation of adenylate cyclase activity; TAS:MGI.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IDA:BHF-UCL.
DR GO; GO:0046034; P:ATP metabolic process; ISO:MGI.
DR GO; GO:0001662; P:behavioral fear response; ISO:MGI.
DR GO; GO:0019933; P:cAMP-mediated signaling; ISS:UniProtKB.
DR GO; GO:0071385; P:cellular response to glucocorticoid stimulus; IEA:Ensembl.
DR GO; GO:0001821; P:histamine secretion; ISO:MGI.
DR GO; GO:0030073; P:insulin secretion; IDA:UniProtKB.
DR GO; GO:0002865; P:negative regulation of acute inflammatory response to antigenic stimulus; ISO:MGI.
DR GO; GO:0002878; P:negative regulation of acute inflammatory response to non-antigenic stimulus; ISO:MGI.
DR GO; GO:0045786; P:negative regulation of cell cycle; IDA:MGI.
DR GO; GO:0060253; P:negative regulation of glial cell proliferation; ISO:MGI.
DR GO; GO:0034260; P:negative regulation of GTPase activity; ISO:MGI.
DR GO; GO:0010656; P:negative regulation of muscle cell apoptotic process; ISO:MGI.
DR GO; GO:0043267; P:negative regulation of potassium ion transport; ISO:MGI.
DR GO; GO:0031175; P:neuron projection development; ISS:UniProtKB.
DR GO; GO:0007218; P:neuropeptide signaling pathway; ISS:UniProtKB.
DR GO; GO:0001541; P:ovarian follicle development; ISO:MGI.
DR GO; GO:0021983; P:pituitary gland development; IEA:Ensembl.
DR GO; GO:0043950; P:positive regulation of cAMP-mediated signaling; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0071651; P:positive regulation of chemokine (C-C motif) ligand 5 production; ISO:MGI.
DR GO; GO:0120162; P:positive regulation of cold-induced thermogenesis; IMP:YuBioLab.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISS:UniProtKB.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISS:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IDA:MGI.
DR GO; GO:0060124; P:positive regulation of growth hormone secretion; ISS:UniProtKB.
DR GO; GO:0043547; P:positive regulation of GTPase activity; ISS:UniProtKB.
DR GO; GO:0032755; P:positive regulation of interleukin-6 production; ISO:MGI.
DR GO; GO:0010976; P:positive regulation of neuron projection development; ISO:MGI.
DR GO; GO:0045860; P:positive regulation of protein kinase activity; ISS:UniProtKB.
DR GO; GO:0090274; P:positive regulation of somatostatin secretion; ISO:MGI.
DR GO; GO:0051968; P:positive regulation of synaptic transmission, glutamatergic; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0008277; P:regulation of G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0070445; P:regulation of oligodendrocyte progenitor proliferation; ISO:MGI.
DR GO; GO:0060078; P:regulation of postsynaptic membrane potential; ISO:MGI.
DR GO; GO:0032880; P:regulation of protein localization; IDA:BHF-UCL.
DR GO; GO:0001932; P:regulation of protein phosphorylation; ISO:MGI.
DR GO; GO:0045471; P:response to ethanol; IEA:Ensembl.
DR GO; GO:0042594; P:response to starvation; IEA:Ensembl.
DR GO; GO:0019233; P:sensory perception of pain; ISO:MGI.
DR GO; GO:0042311; P:vasodilation; ISO:MGI.
DR InterPro; IPR015550; Glucagon.
DR InterPro; IPR000532; Glucagon_GIP_secretin_VIP.
DR InterPro; IPR039080; PACAP.
DR PANTHER; PTHR11213; PTHR11213; 1.
DR PANTHER; PTHR11213:SF1; PTHR11213:SF1; 1.
DR Pfam; PF00123; Hormone_2; 2.
DR PRINTS; PR00275; GLUCAGON.
DR SMART; SM00070; GLUCA; 2.
DR PROSITE; PS00260; GLUCAGON; 1.
PE 2: Evidence at transcript level;
KW Amidation; Cleavage on pair of basic residues; Hormone; Neurogenesis;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT PROPEP 25..78
FT /id="PRO_0000011491"
FT PEPTIDE 81..128
FT /note="PACAP-related peptide"
FT /id="PRO_0000011492"
FT PEPTIDE 131..168
FT /note="Pituitary adenylate cyclase-activating polypeptide
FT 38"
FT /id="PRO_0000011493"
FT PEPTIDE 131..157
FT /note="Pituitary adenylate cyclase-activating polypeptide
FT 27"
FT /id="PRO_0000011494"
FT PROPEP 172..175
FT /id="PRO_0000011495"
FT REGION 149..157
FT /note="Important for receptor binding"
FT /evidence="ECO:0000250"
FT MOD_RES 157
FT /note="Leucine amide"
FT /evidence="ECO:0000250|UniProtKB:P18509"
FT MOD_RES 168
FT /note="Lysine amide"
FT /evidence="ECO:0000250|UniProtKB:P18509"
SQ SEQUENCE 175 AA; 19381 MW; D0E2007DB0C6E8C2 CRC64;
MTMCSGARLA LLVYGIIMHS SVSCSPAAGL SFPGIRPEDE AYDQDGNPLQ DFYDWDPPGV
GSPASALRDA YALYYPADRR DVAHEILNEA YRKVLDQLSA RKYLQSVVAR GAGENLGGSA
VDDPAPLTKR HSDGIFTDSY SRYRKQMAVK KYLAAVLGKR YKQRVKNKGR RIAYL
