ASK1_SCHPO
ID ASK1_SCHPO Reviewed; 307 AA.
AC Q9P6S5; P78949;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=DASH complex subunit ask1;
DE AltName: Full=Associated with spindles and kinetochores protein 1;
DE AltName: Full=Meiotically up-regulated gene 181 protein;
DE AltName: Full=Outer kinetochore protein ask1;
GN Name=ask1; Synonyms=mug181; ORFNames=SPBC27.02c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RA Kohnosu A., Niwa O., Yano M., Saitoh S., Katayama T., Nagao K.,
RA Yanagida M.;
RT "S.pombe chromosome II cosmid 1228 sequence.";
RL Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP FUNCTION IN MEIOSIS.
RX PubMed=16303567; DOI=10.1016/j.cub.2005.10.038;
RA Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L.,
RA Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P.,
RA Smith G.R., Moreno S.;
RT "A large-scale screen in S. pombe identifies seven novel genes required for
RT critical meiotic events.";
RL Curr. Biol. 15:2056-2062(2005).
RN [4]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND PHOSPHORYLATION.
RX PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT "Molecular analysis of kinetochore architecture in fission yeast.";
RL EMBO J. 24:2919-2930(2005).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-136, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Component of the DASH complex, a microtubule-binding
CC subcomplex of the outer kinetochore that is essential for proper
CC chromosome segregation. The DASH complex mediates the formation and
CC maintenance of bipolar kinetochore-microtubule attachments by forming
CC closed rings around spindle microtubules and establishing interactions
CC with proteins from the central kinetochore. Plays a role in meiosis.
CC {ECO:0000269|PubMed:16079914, ECO:0000269|PubMed:16303567}.
CC -!- SUBUNIT: The DASH complex oligomerizes to form rings that encircle the
CC microtubules. {ECO:0000269|PubMed:16079914}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm, cytoskeleton, spindle.
CC Chromosome, centromere, kinetochore. Note=Associates with the mitotic
CC spindle and the kinetochore. Kinetochore association occurs only during
CC mitosis.
CC -!- SIMILARITY: Belongs to the DASH complex ASK1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA12199.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; D83993; BAA12199.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CU329671; CAB89002.1; -; Genomic_DNA.
DR RefSeq; NP_595657.1; NM_001021551.2.
DR AlphaFoldDB; Q9P6S5; -.
DR SMR; Q9P6S5; -.
DR BioGRID; 277026; 363.
DR STRING; 4896.SPBC27.02c.1; -.
DR iPTMnet; Q9P6S5; -.
DR MaxQB; Q9P6S5; -.
DR PaxDb; Q9P6S5; -.
DR PRIDE; Q9P6S5; -.
DR EnsemblFungi; SPBC27.02c.1; SPBC27.02c.1:pep; SPBC27.02c.
DR GeneID; 2540498; -.
DR KEGG; spo:SPBC27.02c; -.
DR PomBase; SPBC27.02c; ask1.
DR VEuPathDB; FungiDB:SPBC27.02c; -.
DR eggNOG; ENOG502RZQN; Eukaryota.
DR HOGENOM; CLU_906607_0_0_1; -.
DR InParanoid; Q9P6S5; -.
DR OMA; CNNIWES; -.
DR PhylomeDB; Q9P6S5; -.
DR PRO; PR:Q9P6S5; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0042729; C:DASH complex; IDA:PomBase.
DR GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR GO; GO:1990941; C:mitotic spindle kinetochore microtubule; IDA:PomBase.
DR GO; GO:1990537; C:mitotic spindle polar microtubule; IDA:PomBase.
DR GO; GO:0044732; C:mitotic spindle pole body; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0043622; P:cortical microtubule organization; IGI:PomBase.
DR GO; GO:1990758; P:mitotic sister chromatid biorientation; TAS:PomBase.
DR GO; GO:0051455; P:monopolar spindle attachment to meiosis I kinetochore; IC:PomBase.
DR InterPro; IPR013964; DASH_Ask1.
DR PANTHER; PTHR28200; PTHR28200; 3.
DR Pfam; PF08655; DASH_Ask1; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW Cytoplasm; Cytoskeleton; Kinetochore; Meiosis; Microtubule; Mitosis;
KW Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..307
FT /note="DASH complex subunit ask1"
FT /id="PRO_0000211316"
FT REGION 117..137
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 250..307
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..292
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 293..307
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 136
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 307 AA; 34593 MW; 6C0A1C7A765C96F3 CRC64;
MNNLEQLERL EQSITLALYE IDANFSKCHR TVTTKILPIV EKYAKNCNTI WDSSKFWKQF
FEASANVSLS GVEEPVPVES NPSDQDVMSN STEADLQLHT KNEHLEKRHS FVGKSDFPDA
AVQGDNTKNE DFVQSTPKKM DVSLEDISLD DAALTPIPAR MQTPLRKPEN NPHTGRSALL
HRVLDTNWQV QVTPREPKNL QSQEVMDIDS SPFVSPSPIS MKMDMPSLND RNSSHALSLF
AEFEHESYDS INPSGMSPPK TIQFSPHTMG VGSSQQANER SLSLQRKLET LNDSNDSFVK
EEDSWEL
