ASK21_ARATH
ID ASK21_ARATH Reviewed; 351 AA.
AC Q8LF97; B3H792;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=SKP1-like protein 21;
DE Short=AtSK21;
GN Name=ASK21; OrderedLocusNames=At3g61415; ORFNames=F2A19.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=12970487; DOI=10.1104/pp.103.024703;
RA Zhao D., Ni W., Feng B., Han T., Petrasek M.G., Ma H.;
RT "Members of the Arabidopsis-SKP1-like gene family exhibit a variety of
RT expression patterns and may play diverse roles in Arabidopsis.";
RL Plant Physiol. 133:203-217(2003).
CC -!- FUNCTION: Involved in ubiquitination and subsequent proteasomal
CC degradation of target proteins. Together with CUL1, RBX1 and a F-box
CC protein, it forms a SCF E3 ubiquitin ligase complex. The functional
CC specificity of this complex depends on the type of F-box protein. In
CC the SCF complex, it serves as an adapter that links the F-box protein
CC to CUL1 (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8LF97-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8LF97-2; Sequence=VSP_037368;
CC -!- TISSUE SPECIFICITY: Expressed in young seedlings, roots, leaves, floral
CC stems, inflorescences, and siliques. {ECO:0000269|PubMed:12970487}.
CC -!- SIMILARITY: Belongs to the SKP1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL132962; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002686; AEE80198.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE80199.1; -; Genomic_DNA.
DR EMBL; AY084971; AAM61531.1; -; mRNA.
DR RefSeq; NP_001118876.1; NM_001125404.1. [Q8LF97-2]
DR RefSeq; NP_567113.1; NM_116006.4. [Q8LF97-1]
DR AlphaFoldDB; Q8LF97; -.
DR SMR; Q8LF97; -.
DR ComplexPortal; CPX-1448; SCF(COI1) ubiquitin ligase complex, variant CUL1-RBX1A-ASK21.
DR ComplexPortal; CPX-1469; SCF(COI1) ubiquitin ligase complex, variant CUL1-RBX1B-ASK21.
DR ComplexPortal; CPX-1491; SCF(COI1) ubiquitin ligase complex, variant CUL2-RBX1A-ASK21.
DR ComplexPortal; CPX-1514; SCF(COI1) ubiquitin ligase complex, variant CUL2-RBX1B-ASK21.
DR ComplexPortal; CPX-1534; SCF(TIR1) ubiquitin ligase complex, variant CUL1-RBX1A-ASK21.
DR ComplexPortal; CPX-1555; SCF(TIR1) ubiquitin ligase complex, variant CUL1-RBX1B-ASK21.
DR ComplexPortal; CPX-1577; SCF(TIR1) ubiquitin ligase complex, variant CUL2-RBX1A-ASK21.
DR ComplexPortal; CPX-1598; SCF(TIR1) ubiquitin ligase complex, variant CUL2-RBX1B-ASK21.
DR STRING; 3702.AT3G61415.1; -.
DR PaxDb; Q8LF97; -.
DR PRIDE; Q8LF97; -.
DR ProteomicsDB; 246793; -. [Q8LF97-1]
DR EnsemblPlants; AT3G61415.1; AT3G61415.1; AT3G61415. [Q8LF97-1]
DR EnsemblPlants; AT3G61415.2; AT3G61415.2; AT3G61415. [Q8LF97-2]
DR GeneID; 825314; -.
DR Gramene; AT3G61415.1; AT3G61415.1; AT3G61415. [Q8LF97-1]
DR Gramene; AT3G61415.2; AT3G61415.2; AT3G61415. [Q8LF97-2]
DR KEGG; ath:AT3G61415; -.
DR Araport; AT3G61415; -.
DR TAIR; locus:505006407; AT3G61415.
DR eggNOG; KOG1724; Eukaryota.
DR HOGENOM; CLU_039678_0_0_1; -.
DR InParanoid; Q8LF97; -.
DR OMA; VWPERIQ; -.
DR OrthoDB; 987444at2759; -.
DR PhylomeDB; Q8LF97; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q8LF97; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8LF97; baseline and differential.
DR Genevisible; Q8LF97; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; ISS:TAIR.
DR GO; GO:0097602; F:cullin family protein binding; IBA:GO_Central.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IC:ComplexPortal.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IMP:ComplexPortal.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0009733; P:response to auxin; IC:ComplexPortal.
DR GO; GO:0009753; P:response to jasmonic acid; IMP:ComplexPortal.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR016897; SKP1.
DR InterPro; IPR001232; SKP1-like.
DR InterPro; IPR036296; SKP1-like_dim_sf.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR InterPro; IPR016072; Skp1_comp_dimer.
DR PANTHER; PTHR11165; PTHR11165; 1.
DR Pfam; PF01466; Skp1; 1.
DR SMART; SM00512; Skp1; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR SUPFAM; SSF81382; SSF81382; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Nucleus; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..351
FT /note="SKP1-like protein 21"
FT /id="PRO_0000375262"
FT REGION 108..167
FT /note="Interaction with the F-box domain of F-box proteins"
FT /evidence="ECO:0000250"
FT REGION 216..240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 330..351
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 345..351
FT /note="GPAAGHK -> EIGHT (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_037368"
SQ SEQUENCE 351 AA; 40022 MW; FCCDD56CCEA0CA6C CRC64;
MSEGEMAIIK PEMMKSYIWL ETADGSIQQV EQEVAMFCPM ICQEVIQKGV GSSKNYAISL
PQRVNPAMLS LIFDYCRFHQ VPGRSNKERK VYDEKFIRMD TKRLCELTSA ADSLQLKPLV
DLTSRALARI IEGKTPEEIR EIFHLPDDLT EEEKLEPLKN TMDDPRIRLL NRLYAKKRKE
LKEREKLKSV EVEEHVDERS VDDLLSFING RDPKVVKTSK SKKKNKKRKE QKNGSSNGTC
EALEKDLHNL DSKSQSAEIV DNTASCLGDV SNLPSMEDDI FTPKTEFEDG YIDDEIDPAL
KELLDREVED FARRLNSSWV LSIGQERQPV NFSINGNGTS RRLTGPAAGH K
