PAC_ARATH
ID PAC_ARATH Reviewed; 313 AA.
AC Q39089; A8MQP8; Q39175; Q7DLY1;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Protein PALE CRESS, chloroplastic;
DE Flags: Precursor;
GN Name=PAC; Synonyms=t9j23; OrderedLocusNames=At2g48120; ORFNames=F11L15.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DISRUPTION PHENOTYPE,
RP INDUCTION BY LIGHT, AND ALTERNATIVE SPLICING.
RC STRAIN=cv. Wassilewskija;
RX PubMed=7919990; DOI=10.2307/3869823;
RA Reiter R.S., Coomber S.A., Bourett T.M., Bartley G.E., Scolnik P.A.;
RT "Control of leaf and chloroplast development by the Arabidopsis gene pale
RT cress.";
RL Plant Cell 6:1253-1264(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), FUNCTION, DISRUPTION
RP PHENOTYPE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=8709959; DOI=10.1007/bf02173642;
RA Grevelding C., Suter-Crazzolara C., Menges A., von Kempner E.,
RA Masterson R., Schell J., Reiss B.;
RT "Characterisation of a new allele of pale cress and its role in greening in
RT Arabidopsis thaliana.";
RL Mol. Gen. Genet. 251:532-541(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=9648738; DOI=10.1007/s004380050740;
RA Meurer J., Grevelding C., Westhoff P., Reiss B.;
RT "The PAC protein affects the maturation of specific chloroplast mRNAs in
RT Arabidopsis thaliana.";
RL Mol. Gen. Genet. 258:342-351(1998).
RN [9]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=10350211; DOI=10.1016/s0171-9335(99)80056-9;
RA Tirlapur U.K., Dahse I., Reiss B., Meurer J., Oelmueller R.;
RT "Characterization of the activity of a plastid-targeted green fluorescent
RT protein in Arabidopsis.";
RL Eur. J. Cell Biol. 78:233-240(1999).
CC -!- FUNCTION: Required for the differentiation of chloroplast from
CC proplastids or etioplasts, probably by modulating some chloroplast-
CC encoded genes expression and mRNA maturation. Involved in leaf-cells
CC differentiation. {ECO:0000269|PubMed:7919990,
CC ECO:0000269|PubMed:8709959, ECO:0000269|PubMed:9648738}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:10350211, ECO:0000269|PubMed:9648738}. Plastid,
CC chromoplast {ECO:0000269|PubMed:10350211}. Plastid, etioplast
CC {ECO:0000269|PubMed:10350211}. Plastid, amyloplast
CC {ECO:0000269|PubMed:10350211}. Note=Present in thin tubular projections
CC emanating from plastids and often connecting organelles each other.
CC {ECO:0000269|PubMed:10350211}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Additional isoforms seem to exist.;
CC Name=1;
CC IsoId=Q39089-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q39089-2; Sequence=VSP_044528;
CC -!- TISSUE SPECIFICITY: Expressed in green tissues, including leaves.
CC Accumulates in chloroplasts of mature stomatal guard cells.
CC {ECO:0000269|PubMed:10350211, ECO:0000269|PubMed:8709959}.
CC -!- INDUCTION: By light. {ECO:0000269|PubMed:7919990}.
CC -!- DISRUPTION PHENOTYPE: Impaired chloroplast development from proplastids
CC or etioplasts leading to reduced chlorophylls and carotenoids
CC accumulation. Reduced accumulation and maturation of some chloroplast-
CC encoded transcripts. Leaves are pale with enlarged intercellular
CC spaces, and loss of differentiation between palisade and mesophyll
CC layers. Partially restored by cytokinin treatment.
CC {ECO:0000269|PubMed:7919990, ECO:0000269|PubMed:8709959,
CC ECO:0000269|PubMed:9648738}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA21761.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; L35241; AAA21761.1; ALT_FRAME; mRNA.
DR EMBL; X96480; CAA65334.1; -; Genomic_DNA.
DR EMBL; X96482; CAA65337.1; -; mRNA.
DR EMBL; AC007166; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002685; AEC10939.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10940.1; -; Genomic_DNA.
DR EMBL; AK117768; BAC42415.1; -; mRNA.
DR EMBL; BT005304; AAO63368.1; -; mRNA.
DR EMBL; AY086817; AAM63866.1; -; mRNA.
DR PIR; F84923; F84923.
DR RefSeq; NP_001078079.1; NM_001084610.1. [Q39089-2]
DR RefSeq; NP_182332.1; NM_130379.2. [Q39089-1]
DR AlphaFoldDB; Q39089; -.
DR SMR; Q39089; -.
DR BioGRID; 4759; 1.
DR IntAct; Q39089; 1.
DR STRING; 3702.AT2G48120.1; -.
DR iPTMnet; Q39089; -.
DR PaxDb; Q39089; -.
DR PRIDE; Q39089; -.
DR ProteomicsDB; 248628; -. [Q39089-1]
DR EnsemblPlants; AT2G48120.1; AT2G48120.1; AT2G48120. [Q39089-1]
DR EnsemblPlants; AT2G48120.2; AT2G48120.2; AT2G48120. [Q39089-2]
DR GeneID; 819424; -.
DR Gramene; AT2G48120.1; AT2G48120.1; AT2G48120. [Q39089-1]
DR Gramene; AT2G48120.2; AT2G48120.2; AT2G48120. [Q39089-2]
DR KEGG; ath:AT2G48120; -.
DR Araport; AT2G48120; -.
DR TAIR; locus:2039361; AT2G48120.
DR eggNOG; ENOG502QVUP; Eukaryota.
DR HOGENOM; CLU_071161_0_0_1; -.
DR InParanoid; Q39089; -.
DR OMA; KACKKRM; -.
DR PhylomeDB; Q39089; -.
DR PRO; PR:Q39089; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q39089; baseline and differential.
DR Genevisible; Q39089; AT.
DR GO; GO:0009501; C:amyloplast; IDA:TAIR.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0042644; C:chloroplast nucleoid; IDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; IDA:TAIR.
DR GO; GO:0009509; C:chromoplast; IDA:TAIR.
DR GO; GO:0009513; C:etioplast; IDA:TAIR.
DR GO; GO:0009537; C:proplastid; IDA:TAIR.
DR GO; GO:0043621; F:protein self-association; IDA:TAIR.
DR GO; GO:0003723; F:RNA binding; IDA:TAIR.
DR GO; GO:0019843; F:rRNA binding; IDA:TAIR.
DR GO; GO:0071482; P:cellular response to light stimulus; IEP:UniProtKB.
DR GO; GO:0010239; P:chloroplast mRNA processing; IMP:TAIR.
DR GO; GO:0009658; P:chloroplast organization; IMP:TAIR.
DR GO; GO:0009965; P:leaf morphogenesis; IMP:TAIR.
DR GO; GO:0042254; P:ribosome biogenesis; IMP:TAIR.
DR GO; GO:0016070; P:RNA metabolic process; IMP:TAIR.
DR InterPro; IPR034563; PALE_CRESS.
DR PANTHER; PTHR37219; PTHR37219; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Amyloplast; Chloroplast; Developmental protein;
KW mRNA processing; Plastid; Reference proteome; Transcription;
KW Transcription regulation; Transit peptide.
FT TRANSIT 1..22
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 23..313
FT /note="Protein PALE CRESS, chloroplastic"
FT /id="PRO_0000420527"
FT VAR_SEQ 62..67
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_044528"
SQ SEQUENCE 313 AA; 36612 MW; E3711AA2A4E975BE CRC64;
MAATSLVLTC ASPLFSSPRV ISATKKLTTE LSISTAKFRR RCSGNNDEVL LEGMPPEYYD
DEWQARQREK TKELRRMQRE EEEEEERKIE EYREIGTRLK EFPEQDLRKA RKLVSSFIRA
AEEVEERIEE AAEKGELDEL VLMIIWNRLD LARRDDEKDA IRSLDLLYRR VETEILKRQA
SPAMKLLNDL LNMHDGFEDD AWLKDCRKRM AETFPREDPF SILMPPGFDI DMHQGQLRPP
IETETDNTLL RVDFVREVDA LLQEVRIEED ATTGSKGEGL DPEAIALKFK QQEKQRTIRQ
IEAILDLALN LKW
