PAC_STRMG
ID PAC_STRMG Reviewed; 1565 AA.
AC P11657;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 25-MAY-2022, entry version 131.
DE RecName: Full=Major cell-surface adhesin PAc {ECO:0000303|PubMed:2761390};
DE AltName: Full=Antigen I/II;
DE Flags: Precursor;
GN Name=pac {ECO:0000303|PubMed:2761390};
OS Streptococcus mutans.
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=1309;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 39-48.
RC STRAIN=MT8148 / Serotype c;
RX PubMed=2761390; DOI=10.1111/j.1365-2958.1989.tb00215.x;
RA Okahashi N., Sasakawa C., Yoshikawa M., Hamada S., Koga T.;
RT "Molecular characterization of a surface protein antigen gene from serotype
RT c Streptococcus mutans, implicated in dental caries.";
RL Mol. Microbiol. 3:673-678(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=MT8148 / Serotype c;
RA Terao Y., Kawabata S., Hamada S.;
RT "Identification of Streptococcus mutans rgtB gene as a regulator of
RT glucosyltransferase expression.";
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 463-839.
RC STRAIN=OMZ175 / Serotype f;
RX PubMed=12054777; DOI=10.1016/s0022-2836(02)00025-6;
RA Troffer-Charlier N., Ogier J., Moras D., Cavarelli J.;
RT "Crystal structure of the V-region of Streptococcus mutans antigen I/II at
RT 2.4 A resolution suggests a sugar preformed binding site.";
RL J. Mol. Biol. 318:179-188(2002).
CC -!- FUNCTION: Surface protein antigen implicated in dental caries.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000255|PROSITE-
CC ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00477}.
CC -!- SIMILARITY: Belongs to the antigen I/II family. {ECO:0000305}.
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DR EMBL; X14490; CAA32652.1; -; Genomic_DNA.
DR EMBL; AB040534; BAC54564.1; -; Genomic_DNA.
DR PIR; S04729; S04729.
DR PDB; 1JMM; X-ray; 2.40 A; A=463-839.
DR PDB; 3OPU; X-ray; 2.18 A; A/B/C/D/E/F=1154-1492.
DR PDB; 3QE5; X-ray; 2.50 A; A/B=991-1485.
DR PDB; 6TZL; X-ray; 1.60 A; A/B/C/D=446-848.
DR PDB; 6UBV; X-ray; 2.70 A; A/B/C/D=446-848.
DR PDBsum; 1JMM; -.
DR PDBsum; 3OPU; -.
DR PDBsum; 3QE5; -.
DR PDBsum; 6TZL; -.
DR PDBsum; 6UBV; -.
DR AlphaFoldDB; P11657; -.
DR BMRB; P11657; -.
DR SMR; P11657; -.
DR STRING; 1198676.SMUGS5_02680; -.
DR eggNOG; COG3064; Bacteria.
DR eggNOG; COG3087; Bacteria.
DR EvolutionaryTrace; P11657; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR Gene3D; 2.60.530.10; -; 1.
DR InterPro; IPR026345; Adh_isopep-form_adh_dom.
DR InterPro; IPR041324; AgI/II_N.
DR InterPro; IPR032300; Antigen_C.
DR InterPro; IPR021197; Cross-wall-target_lipo_motif.
DR InterPro; IPR013574; Glucan-bd_C/Surface_Ag-I/II_V.
DR InterPro; IPR019931; LPXTG_anchor.
DR InterPro; IPR036234; SA_I/II_PAC_V_sf.
DR InterPro; IPR009578; Surface_Ag_rpt.
DR Pfam; PF18652; Adhesin_P1_N; 1.
DR Pfam; PF17998; AgI_II_C2; 1.
DR Pfam; PF16364; Antigen_C; 1.
DR Pfam; PF08363; GbpC; 1.
DR Pfam; PF00746; Gram_pos_anchor; 1.
DR Pfam; PF06696; Strep_SA_rep; 7.
DR SUPFAM; SSF74914; SSF74914; 1.
DR TIGRFAMs; TIGR04228; isopep_sspB_C2; 1.
DR TIGRFAMs; TIGR03726; strep_RK_lipo; 1.
DR PROSITE; PS51965; AG_I_II_AR; 4.
DR PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell wall; Dental caries; Direct protein sequencing;
KW Peptidoglycan-anchor; Repeat; Secreted; Signal.
FT SIGNAL 1..38
FT /evidence="ECO:0000269|PubMed:2761390"
FT CHAIN 39..1535
FT /note="Major cell-surface adhesin PAc"
FT /id="PRO_0000005645"
FT PROPEP 1536..1565
FT /note="Removed by sortase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT /id="PRO_0000005646"
FT REPEAT 146..220
FT /note="Ag I/II A 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01310"
FT REPEAT 221..302
FT /note="Ag I/II A 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01310"
FT REPEAT 303..384
FT /note="Ag I/II A 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01310"
FT REPEAT 385..466
FT /note="Ag I/II A 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01310"
FT REPEAT 848..887
FT /note="P1"
FT REPEAT 888..926
FT /note="P2"
FT REPEAT 927..964
FT /note="P3"
FT REGION 42..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 203..448
FT /note="Heptad repeats of Y-[EQ]-X-X-L-A-X"
FT REGION 461..834
FT /note="V-region (lectin-like)"
FT REGION 827..985
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1486..1511
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 1532..1536
FT /note="LPXTG sorting signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT COMPBIAS 42..75
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 861..892
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 900..931
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 939..983
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1535
FT /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT HELIX 446..454
FT /evidence="ECO:0007829|PDB:6TZL"
FT HELIX 458..490
FT /evidence="ECO:0007829|PDB:6TZL"
FT HELIX 491..493
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 499..502
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 517..527
FT /evidence="ECO:0007829|PDB:6TZL"
FT HELIX 529..536
FT /evidence="ECO:0007829|PDB:6TZL"
FT HELIX 539..541
FT /evidence="ECO:0007829|PDB:6TZL"
FT HELIX 542..545
FT /evidence="ECO:0007829|PDB:6TZL"
FT TURN 546..548
FT /evidence="ECO:0007829|PDB:6TZL"
FT HELIX 552..554
FT /evidence="ECO:0007829|PDB:6TZL"
FT HELIX 557..560
FT /evidence="ECO:0007829|PDB:6TZL"
FT HELIX 563..565
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 566..569
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 572..576
FT /evidence="ECO:0007829|PDB:6TZL"
FT HELIX 577..579
FT /evidence="ECO:0007829|PDB:6TZL"
FT TURN 585..587
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 593..599
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 604..610
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 624..631
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 638..640
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 642..649
FT /evidence="ECO:0007829|PDB:6TZL"
FT HELIX 650..652
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 654..657
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 664..666
FT /evidence="ECO:0007829|PDB:1JMM"
FT STRAND 668..678
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 687..694
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 703..709
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 711..715
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 721..725
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 728..731
FT /evidence="ECO:0007829|PDB:6TZL"
FT HELIX 741..743
FT /evidence="ECO:0007829|PDB:6UBV"
FT STRAND 751..753
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 759..762
FT /evidence="ECO:0007829|PDB:6TZL"
FT HELIX 766..769
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 771..778
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 780..788
FT /evidence="ECO:0007829|PDB:6TZL"
FT HELIX 789..791
FT /evidence="ECO:0007829|PDB:6TZL"
FT HELIX 795..797
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 812..816
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 818..820
FT /evidence="ECO:0007829|PDB:6TZL"
FT STRAND 824..826
FT /evidence="ECO:0007829|PDB:1JMM"
FT STRAND 1003..1009
FT /evidence="ECO:0007829|PDB:3QE5"
FT STRAND 1025..1032
FT /evidence="ECO:0007829|PDB:3QE5"
FT STRAND 1045..1050
FT /evidence="ECO:0007829|PDB:3QE5"
FT STRAND 1055..1057
FT /evidence="ECO:0007829|PDB:3QE5"
FT HELIX 1059..1064
FT /evidence="ECO:0007829|PDB:3QE5"
FT STRAND 1069..1074
FT /evidence="ECO:0007829|PDB:3QE5"
FT HELIX 1075..1077
FT /evidence="ECO:0007829|PDB:3QE5"
FT STRAND 1079..1084
FT /evidence="ECO:0007829|PDB:3QE5"
FT HELIX 1086..1093
FT /evidence="ECO:0007829|PDB:3QE5"
FT TURN 1094..1097
FT /evidence="ECO:0007829|PDB:3QE5"
FT STRAND 1106..1111
FT /evidence="ECO:0007829|PDB:3QE5"
FT STRAND 1117..1120
FT /evidence="ECO:0007829|PDB:3QE5"
FT STRAND 1123..1126
FT /evidence="ECO:0007829|PDB:3QE5"
FT TURN 1127..1129
FT /evidence="ECO:0007829|PDB:3QE5"
FT STRAND 1130..1133
FT /evidence="ECO:0007829|PDB:3QE5"
FT STRAND 1137..1140
FT /evidence="ECO:0007829|PDB:3QE5"
FT STRAND 1159..1161
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1180..1187
FT /evidence="ECO:0007829|PDB:3OPU"
FT HELIX 1190..1192
FT /evidence="ECO:0007829|PDB:3OPU"
FT HELIX 1199..1202
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1206..1211
FT /evidence="ECO:0007829|PDB:3OPU"
FT TURN 1214..1216
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1217..1219
FT /evidence="ECO:0007829|PDB:3OPU"
FT HELIX 1221..1223
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1225..1228
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1236..1244
FT /evidence="ECO:0007829|PDB:3OPU"
FT TURN 1245..1247
FT /evidence="ECO:0007829|PDB:3OPU"
FT HELIX 1250..1258
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1267..1273
FT /evidence="ECO:0007829|PDB:3OPU"
FT HELIX 1275..1282
FT /evidence="ECO:0007829|PDB:3OPU"
FT TURN 1283..1286
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1289..1297
FT /evidence="ECO:0007829|PDB:3OPU"
FT HELIX 1299..1304
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1306..1316
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1319..1330
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1336..1342
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1361..1367
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1373..1377
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1382..1387
FT /evidence="ECO:0007829|PDB:3OPU"
FT TURN 1390..1392
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1393..1405
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1407..1409
FT /evidence="ECO:0007829|PDB:3OPU"
FT HELIX 1422..1424
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1425..1430
FT /evidence="ECO:0007829|PDB:3OPU"
FT TURN 1431..1434
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1435..1440
FT /evidence="ECO:0007829|PDB:3OPU"
FT HELIX 1442..1447
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1456..1464
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1466..1472
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1474..1478
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1481..1484
FT /evidence="ECO:0007829|PDB:3OPU"
FT STRAND 1488..1492
FT /evidence="ECO:0007829|PDB:3OPU"
SQ SEQUENCE 1565 AA; 170782 MW; 4C3B05C809D0C32A CRC64;
MKVKKTYGFR KSKISKTLCG AVLGTVAAVS VAGQKVFADE TTTTSDVDTK VVGTQTGNPA
TNLPEAQGSA SKEAEQSQTK LERQMVHTIE VPKTDLDQAA KDAKSAGVNV VQDADVNKGT
VKTPEEAVQK ETEIKEDYTK QAEDIKKTTD QYKSDVAAHE AEVAKIKAKN QATKEQYEKD
MAAHKAEVER INAANAASKT AYEAKLAQYQ ADLAAVQKTN AANQAAYQKA LAAYQAELKR
VQEANAAAKA AYDTAVAANN AKNTEIAAAN EEIRKRNATA KAEYETKLAQ YQAELKRVQE
ANAANEADYQ AKLTAYQTEL ARVQKANADA KATYEAAVAA NNAKNAALTA ENTAIKQRNE
NAKATYEAAL KQYEADLAAV KKANAANEAD YQAKLTAYQT ELARVQKANA DAKAAYEAAV
AANNAANAAL TAENTAIKKR NADAKADYEA KLAKYQADLA KYQKDLADYP VKLKAYEDEQ
TSIKAALAEL EKHKNEDGNL TEPSAQNLVY DLEPNANLSL TTDGKFLKAS AVDDAFSKST
SKAKYDQKIL QLDDLDITNL EQSNDVASSM ELYGNFGDKA GWSTTVSNNS QVKWGSVLLE
RGQSATATYT NLQNSYYNGK KISKIVYKYT VDPKSKFQGQ KVWLGIFTDP TLGVFASAYT
GQVEKNTSIF IKNEFTFYHE DEKPINFDNA LLSVTSLNRE HNSIEMAKDY SGKFVKISGS
SIGEKNGMIY ATDTLNFKQG EGGSRWTMYK NSQAGSGWDS SDAPNSWYGA GAIKMSGPNN
HVTVGATSAT NVMPVSDMPV VPGKDNTDGK KPNIWYSLNG KIRAVNVPKV TKEKPTPPVK
PTAPTKPTYE TEKPLKPAPV APNYEKEPTP PTRTPDQAEP NKPTPPTYET EKPLEPAPVE
PSYEAEPTPP TRTPDQAEPN KPTPPTYETE KPLEPAPVEP SYEAEPTPPT PTPDQPEPNK
PVEPTYEVIP TPPTDPVYQD LPTPPSDPTV HFHYFKLAVQ PQVNKEIRNN NDINIDRTLV
AKQSVVKFQL KTADLPAGRD ETTSFVLVDP LPSGYQFNPE ATKAASPGFD VTYDNATNTV
TFKATAATLA TFNADLTKSV ATIYPTVVGQ VLNDGATYKN NFTLTVNDAY GIKSNVVRVT
TPGKPNDPDN PNNNYIKPTK VNKNENGVVI DGKTVLAGST NYYELTWDLD QYKNDRSSAD
TIQKGFYYVD DYPEEALELR QDLVKITDAN GNEVTGVSVD NYTNLEAAPQ EIRDVLSKAG
IRPKGAFQIF RADNPREFYD TYVKTGIDLK IVSPMVVKKQ MGQTGGSYEN QAYQIDFGNG
YASNIVINNV PKINPKKDVT LTLDPADTNN VDGQTIPLNT VFNYRLIGGI IPANHSEELF
EYNFYDDYDQ TGDHYTGQYK VFAKVDITLK NGVIIKSGTE LTQYTTAEVD TTKGAITIKF
KEAFLRSVSI DSAFQAESYI QMKRIAVGTF ENTYINTVNG VTYSSNTVKT TTPEDPADPT
DPQDPSSPRT STVIIYKPQS TAYQPSSVQE TLPNTGVTNN AYMPLLGIIG LVTSFSLLGL
KAKKD
