PADC1_HUMAN
ID PADC1_HUMAN Reviewed; 188 AA.
AC Q9BSG0; Q2Z1P2;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Protease-associated domain-containing protein 1;
DE AltName: Full=Protease-associated domain-containing protein of 21 kDa;
DE Short=hPAP21;
DE Flags: Precursor;
GN Name=PRADC1 {ECO:0000312|HGNC:HGNC:16047}; Synonyms=C2orf7, PAP21;
GN ORFNames=UNQ833/PRO1760;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Skeletal muscle;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION AT ASN-171,
RP PROBABLE LACK OF GLYCOSYLATION AT ASN-121, AND MUTAGENESIS OF ASN-121 AND
RP ASN-171.
RX PubMed=15498570; DOI=10.1016/j.febslet.2004.09.039;
RA Zhou Y.-B., Liu F., Zhu Z.-D., Zhu H., Zhang X., Wang Z.-Q., Liu J.-H.,
RA Han Z.-G.;
RT "N-glycosylation is required for efficient secretion of a novel human
RT secreted glycoprotein, hPAP21.";
RL FEBS Lett. 576:401-407(2004).
CC -!- FUNCTION: Plays a role in the modulation of physical activity and
CC adiposity. {ECO:0000250|UniProtKB:Q9D9N8}.
CC -!- INTERACTION:
CC Q9BSG0; Q08AM6: VAC14; NbExp=3; IntAct=EBI-10297527, EBI-2107455;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15498570}.
CC -!- TISSUE SPECIFICITY: Highly expressed in skeletal muscle, heart and
CC liver. Expressed at intermediate level in kidney.
CC {ECO:0000269|PubMed:15498570}.
CC -!- PTM: N-glycosylated; required for efficient secretion.
CC {ECO:0000269|PubMed:15498570}.
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DR EMBL; AY358605; AAQ88968.1; -; mRNA.
DR EMBL; AK312077; BAG35013.1; -; mRNA.
DR EMBL; AC010913; AAX88901.1; -; Genomic_DNA.
DR EMBL; CH471053; EAW99742.1; -; Genomic_DNA.
DR EMBL; CH471053; EAW99743.1; -; Genomic_DNA.
DR EMBL; BC005069; AAH05069.1; -; mRNA.
DR CCDS; CCDS1924.1; -.
DR RefSeq; NP_115695.1; NM_032319.2.
DR AlphaFoldDB; Q9BSG0; -.
DR SMR; Q9BSG0; -.
DR BioGRID; 124006; 9.
DR IntAct; Q9BSG0; 5.
DR STRING; 9606.ENSP00000258083; -.
DR GlyGen; Q9BSG0; 1 site.
DR iPTMnet; Q9BSG0; -.
DR PhosphoSitePlus; Q9BSG0; -.
DR BioMuta; PRADC1; -.
DR DMDM; 55976581; -.
DR EPD; Q9BSG0; -.
DR jPOST; Q9BSG0; -.
DR MassIVE; Q9BSG0; -.
DR MaxQB; Q9BSG0; -.
DR PaxDb; Q9BSG0; -.
DR PeptideAtlas; Q9BSG0; -.
DR PRIDE; Q9BSG0; -.
DR ProteomicsDB; 78887; -.
DR Antibodypedia; 31326; 16 antibodies from 8 providers.
DR DNASU; 84279; -.
DR Ensembl; ENST00000258083.3; ENSP00000258083.2; ENSG00000135617.4.
DR GeneID; 84279; -.
DR KEGG; hsa:84279; -.
DR MANE-Select; ENST00000258083.3; ENSP00000258083.2; NM_032319.3; NP_115695.1.
DR UCSC; uc002siy.3; human.
DR CTD; 84279; -.
DR DisGeNET; 84279; -.
DR GeneCards; PRADC1; -.
DR HGNC; HGNC:16047; PRADC1.
DR HPA; ENSG00000135617; Tissue enhanced (skeletal).
DR MIM; 619674; gene.
DR neXtProt; NX_Q9BSG0; -.
DR OpenTargets; ENSG00000135617; -.
DR PharmGKB; PA25895; -.
DR VEuPathDB; HostDB:ENSG00000135617; -.
DR eggNOG; KOG3920; Eukaryota.
DR GeneTree; ENSGT00390000009837; -.
DR HOGENOM; CLU_084006_2_0_1; -.
DR InParanoid; Q9BSG0; -.
DR OMA; KINHPPW; -.
DR OrthoDB; 1302890at2759; -.
DR PhylomeDB; Q9BSG0; -.
DR TreeFam; TF335463; -.
DR PathwayCommons; Q9BSG0; -.
DR SignaLink; Q9BSG0; -.
DR BioGRID-ORCS; 84279; 15 hits in 1076 CRISPR screens.
DR ChiTaRS; PRADC1; human.
DR GenomeRNAi; 84279; -.
DR Pharos; Q9BSG0; Tdark.
DR PRO; PR:Q9BSG0; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q9BSG0; protein.
DR Bgee; ENSG00000135617; Expressed in apex of heart and 159 other tissues.
DR Genevisible; Q9BSG0; HS.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR CDD; cd02127; PA_hPAP21_like; 1.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR042773; PADC1.
DR InterPro; IPR037323; PRADC1-like_PA.
DR PANTHER; PTHR22702; PTHR22702; 1.
DR Pfam; PF02225; PA; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..188
FT /note="Protease-associated domain-containing protein 1"
FT /id="PRO_0000022001"
FT DOMAIN 83..163
FT /note="PA"
FT SITE 121
FT /note="Not glycosylated"
FT /evidence="ECO:0000305"
FT CARBOHYD 171
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:15498570"
FT MUTAGEN 121
FT /note="N->Q: Does not affect glycosylation state. Abolishes
FT N-glycosylation; when associated with Q-171."
FT /evidence="ECO:0000269|PubMed:15498570"
FT MUTAGEN 171
FT /note="N->Q: Abolishes N-glycosylation. Abolishes N-
FT glycosylation; when associated with Q-121."
FT /evidence="ECO:0000269|PubMed:15498570"
SQ SEQUENCE 188 AA; 21042 MW; 372F16BCDF06CAE9 CRC64;
MVPGAAGWCC LVLWLPACVA AHGFRIHDYL YFQVLSPGDI RYIFTATPAK DFGGIFHTRY
EQIHLVPAEP PEACGELSNG FFIQDQIALV ERGGCSFLSK TRVVQEHGGR AVIISDNAVD
NDSFYVEMIQ DSTQRTADIP ALFLLGRDGY MIRRSLEQHG LPWAIISIPV NVTSIPTFEL
LQPPWTFW
