PAE10_ARATH
ID PAE10_ARATH Reviewed; 416 AA.
AC Q66GM8; B9DGF9; O65250; Q0WRK2;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=Pectin acetylesterase 10 {ECO:0000303|PubMed:25115560};
DE EC=3.1.1.- {ECO:0000305};
DE Flags: Precursor;
GN Name=PAE10 {ECO:0000303|PubMed:25115560};
GN OrderedLocusNames=At5g26670 {ECO:0000312|Araport:AT5G26670};
GN ORFNames=F21E10.11 {ECO:0000312|EMBL:AAC13595.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP GENE FAMILY, AND DISRUPTION PHENOTYPE.
RX PubMed=25115560; DOI=10.1007/s00425-014-2139-6;
RA de Souza A., Hull P.A., Gille S., Pauly M.;
RT "Identification and functional characterization of the distinct plant
RT pectin esterases PAE8 and PAE9 and their deletion mutants.";
RL Planta 240:1123-1138(2014).
CC -!- FUNCTION: Hydrolyzes acetyl esters in homogalacturonan regions of
CC pectin. In type I primary cell wall, galacturonic acid residues of
CC pectin can be acetylated at the O-2 and O-3 positions. Decreasing the
CC degree of acetylation of pectin gels in vitro alters their physical
CC properties. {ECO:0000250|UniProtKB:B9DFR3}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q66GM8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q66GM8-2; Sequence=VSP_057379;
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. {ECO:0000269|PubMed:25115560}.
CC -!- SIMILARITY: Belongs to the pectinacetylesterase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC13595.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF058914; AAC13595.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED93578.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93579.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69252.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69253.1; -; Genomic_DNA.
DR EMBL; AK317140; BAH19826.1; -; mRNA.
DR EMBL; BT015374; AAU05497.1; -; mRNA.
DR EMBL; BT029367; ABK32181.1; -; mRNA.
DR EMBL; AK228304; BAF00247.1; -; mRNA.
DR PIR; T01197; T01197.
DR RefSeq; NP_001318657.1; NM_001343974.1. [Q66GM8-2]
DR RefSeq; NP_001330948.1; NM_001343975.1. [Q66GM8-2]
DR RefSeq; NP_850878.2; NM_180547.3. [Q66GM8-1]
DR RefSeq; NP_974837.1; NM_203108.2. [Q66GM8-2]
DR AlphaFoldDB; Q66GM8; -.
DR SMR; Q66GM8; -.
DR STRING; 3702.AT5G26670.1; -.
DR ESTHER; arath-q66gm8; Pectinacetylesterase-Notum.
DR PaxDb; Q66GM8; -.
DR PRIDE; Q66GM8; -.
DR ProteomicsDB; 226045; -. [Q66GM8-1]
DR EnsemblPlants; AT5G26670.1; AT5G26670.1; AT5G26670. [Q66GM8-1]
DR EnsemblPlants; AT5G26670.2; AT5G26670.2; AT5G26670. [Q66GM8-2]
DR EnsemblPlants; AT5G26670.3; AT5G26670.3; AT5G26670. [Q66GM8-2]
DR EnsemblPlants; AT5G26670.4; AT5G26670.4; AT5G26670. [Q66GM8-2]
DR GeneID; 832722; -.
DR Gramene; AT5G26670.1; AT5G26670.1; AT5G26670. [Q66GM8-1]
DR Gramene; AT5G26670.2; AT5G26670.2; AT5G26670. [Q66GM8-2]
DR Gramene; AT5G26670.3; AT5G26670.3; AT5G26670. [Q66GM8-2]
DR Gramene; AT5G26670.4; AT5G26670.4; AT5G26670. [Q66GM8-2]
DR KEGG; ath:AT5G26670; -.
DR Araport; AT5G26670; -.
DR TAIR; locus:2146814; AT5G26670.
DR eggNOG; KOG4287; Eukaryota.
DR InParanoid; Q66GM8; -.
DR OMA; MARVFWT; -.
DR PhylomeDB; Q66GM8; -.
DR PRO; PR:Q66GM8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q66GM8; baseline and differential.
DR Genevisible; Q66GM8; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0052793; F:pectin acetylesterase activity; IBA:GO_Central.
DR GO; GO:0071555; P:cell wall organization; IBA:GO_Central.
DR InterPro; IPR004963; PAE/NOTUM.
DR PANTHER; PTHR21562; PTHR21562; 1.
DR Pfam; PF03283; PAE; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell wall; Cell wall biogenesis/degradation;
KW Glycoprotein; Hydrolase; Reference proteome; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..416
FT /note="Pectin acetylesterase 10"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431775"
FT ACT_SITE 198
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6P988"
FT ACT_SITE 294
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6P988"
FT ACT_SITE 361
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6P988"
FT CARBOHYD 27
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT VAR_SEQ 1..118
FT /note="Missing (in isoform 2)"
FT /id="VSP_057379"
FT CONFLICT 156
FT /note="S -> G (in Ref. 3; BAH19826)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 416 AA; 46562 MW; DFED3E3674873190 CRC64;
MRKLFLLGFI VAGLVLGNEA NGYLEFNVTE LDRIEDLEFG FSKFSSNFNP LMVGLTLIRG
AGSKGAVCLD GTLPGYHLHR GHGSGANSWL IQLEGGGWCD NIRNCVYRKK SRRGSSNYME
KQIQFTGILS NKAQENPDFF NWNRVKLRYC DGGSFSGDSQ NKAARLQFRG EKIWRAAMDD
LKAKGMRNAK QALLSGCSAG GLAVILRCDE FRNLFSGWTR VKCLSDAGLF LDTPDVSGGH
TIRNLYNGVV QLQGVKNNLP HLCTNHLNPT SCFFPQNLIS QMKTPLFIVN AAYDIWQIQS
SIAPPSADPS GYWHECRLNH GRCTPAQIRF LQGFRNQMLR AVSGFSNSKK NGLFINSCFA
HCQTERQDTW FADDSPVIHK KAVAIAVGDW YFDRAEVKLI DCPYPCDRSC HNLVFR
