ASK9_ARATH
ID ASK9_ARATH Reviewed; 153 AA.
AC Q9LSX9; A0MEX6;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=SKP1-like protein 9;
DE Short=AtSK9;
GN Name=ASK9; OrderedLocusNames=At3g21850; ORFNames=MSD21.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12970487; DOI=10.1104/pp.103.024703;
RA Zhao D., Ni W., Feng B., Han T., Petrasek M.G., Ma H.;
RT "Members of the Arabidopsis-SKP1-like gene family exhibit a variety of
RT expression patterns and may play diverse roles in Arabidopsis.";
RL Plant Physiol. 133:203-217(2003).
RN [5]
RP TISSUE SPECIFICITY, AND INTERACTION WITH AT3G61590.
RX PubMed=14749489; DOI=10.1093/pcp/pch009;
RA Takahashi N., Kuroda H., Kuromori T., Hirayama T., Seki M., Shinozaki K.,
RA Shimada H., Matsui M.;
RT "Expression and interaction analysis of Arabidopsis Skp1-related genes.";
RL Plant Cell Physiol. 45:83-91(2004).
RN [6]
RP INTERACTION WITH CPR1/CPR30.
RX PubMed=19682297; DOI=10.1111/j.1365-313x.2009.03995.x;
RA Gou M., Su N., Zheng J., Huai J., Wu G., Zhao J., He J., Tang D., Yang S.,
RA Wang G.;
RT "An F-box gene, CPR30, functions as a negative regulator of the defense
RT response in Arabidopsis.";
RL Plant J. 60:757-770(2009).
CC -!- FUNCTION: Involved in ubiquitination and subsequent proteasomal
CC degradation of target proteins. Together with CUL1, RBX1 and a F-box
CC protein, it forms a SCF E3 ubiquitin ligase complex. The functional
CC specificity of this complex depends on the type of F-box protein. In
CC the SCF complex, it serves as an adapter that links the F-box protein
CC to CUL1 (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By
CC similarity). Interacts with CPR1/CPR30 and At3g61590. {ECO:0000250,
CC ECO:0000269|PubMed:14749489, ECO:0000269|PubMed:19682297}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, shoot apical meristem (SAM),
CC roots, flowers and pollen. {ECO:0000269|PubMed:14749489}.
CC -!- SIMILARITY: Belongs to the SKP1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28567.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB025634; BAB02847.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76558.1; -; Genomic_DNA.
DR EMBL; DQ446683; ABE65955.1; -; mRNA.
DR EMBL; DQ653099; ABK28567.1; ALT_SEQ; mRNA.
DR RefSeq; NP_566694.1; NM_113080.2.
DR AlphaFoldDB; Q9LSX9; -.
DR SMR; Q9LSX9; -.
DR BioGRID; 7071; 37.
DR ComplexPortal; CPX-1436; SCF(COI1) ubiquitin ligase complex, variant CUL1-RBX1A-ASK9.
DR ComplexPortal; CPX-1457; SCF(COI1) ubiquitin ligase complex, variant CUL1-RBX1B-ASK9.
DR ComplexPortal; CPX-1479; SCF(COI1) ubiquitin ligase complex, variant CUL2-RBX1A-ASK9.
DR ComplexPortal; CPX-1502; SCF(COI1) ubiquitin ligase complex, variant CUL2-RBX1B-ASK9.
DR ComplexPortal; CPX-1522; SCF(TIR1) ubiquitin ligase complex, variant CUL1-RBX1A-ASK9.
DR ComplexPortal; CPX-1543; SCF(TIR1) ubiquitin ligase complex, variant CUL1-RBX1B-ASK9.
DR ComplexPortal; CPX-1565; SCF(TIR1) ubiquitin ligase complex, variant CUL2-RBX1A-ASK9.
DR ComplexPortal; CPX-1586; SCF(TIR1) ubiquitin ligase complex, variant CUL2-RBX1B-ASK9.
DR IntAct; Q9LSX9; 10.
DR STRING; 3702.AT3G21850.1; -.
DR PaxDb; Q9LSX9; -.
DR PRIDE; Q9LSX9; -.
DR ProteomicsDB; 246857; -.
DR EnsemblPlants; AT3G21850.1; AT3G21850.1; AT3G21850.
DR GeneID; 821739; -.
DR Gramene; AT3G21850.1; AT3G21850.1; AT3G21850.
DR KEGG; ath:AT3G21850; -.
DR Araport; AT3G21850; -.
DR TAIR; locus:2093084; AT3G21850.
DR eggNOG; KOG1724; Eukaryota.
DR HOGENOM; CLU_059252_6_1_1; -.
DR OMA; WANNTIP; -.
DR OrthoDB; 1412723at2759; -.
DR PhylomeDB; Q9LSX9; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9LSX9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LSX9; baseline and differential.
DR Genevisible; Q9LSX9; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IC:ComplexPortal.
DR GO; GO:0097602; F:cullin family protein binding; IBA:GO_Central.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IC:ComplexPortal.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IMP:ComplexPortal.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0007346; P:regulation of mitotic cell cycle; TAS:TAIR.
DR GO; GO:0009733; P:response to auxin; IC:ComplexPortal.
DR GO; GO:0009753; P:response to jasmonic acid; IMP:ComplexPortal.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR016897; SKP1.
DR InterPro; IPR001232; SKP1-like.
DR InterPro; IPR036296; SKP1-like_dim_sf.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR InterPro; IPR016072; Skp1_comp_dimer.
DR InterPro; IPR016073; Skp1_comp_POZ.
DR PANTHER; PTHR11165; PTHR11165; 1.
DR Pfam; PF01466; Skp1; 1.
DR Pfam; PF03931; Skp1_POZ; 1.
DR PIRSF; PIRSF028729; E3_ubiquit_lig_SCF_Skp; 1.
DR SMART; SM00512; Skp1; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR SUPFAM; SSF81382; SSF81382; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..153
FT /note="SKP1-like protein 9"
FT /id="PRO_0000375250"
FT REGION 95..153
FT /note="Interaction with the F-box domain of F-box proteins"
FT /evidence="ECO:0000250"
SQ SEQUENCE 153 AA; 17439 MW; 7F5965319CFAF4F9 CRC64;
MSTKKIILKS SDGHSFEVEE EAARQCQIII AHMSENDCTD NGIPLPNVTG KILAMVIEYC
NKHHVDAANP CSDDDLKKWD KEFMEKDTST IFDLIKAANY LNIKSLFDLA CQTVAEIIKG
NTPEQIREFF NIENDLTPEE EAAIRRENKW AFE
