PAE8_ARATH
ID PAE8_ARATH Reviewed; 397 AA.
AC Q6DBP4; F4JT68; O65713;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Pectin acetylesterase 8 {ECO:0000303|PubMed:25115560};
DE EC=3.1.1.- {ECO:0000305};
DE Flags: Precursor;
GN Name=PAE8 {ECO:0000303|PubMed:25115560};
GN OrderedLocusNames=At4g19420 {ECO:0000312|Araport:AT4G19420};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=25115560; DOI=10.1007/s00425-014-2139-6;
RA de Souza A., Hull P.A., Gille S., Pauly M.;
RT "Identification and functional characterization of the distinct plant
RT pectin esterases PAE8 and PAE9 and their deletion mutants.";
RL Planta 240:1123-1138(2014).
CC -!- FUNCTION: Hydrolyzes acetyl esters in homogalacturonan regions of
CC pectin. In type I primary cell wall, galacturonic acid residues of
CC pectin can be acetylated at the O-2 and O-3 positions. Decreasing the
CC degree of acetylation of pectin gels in vitro alters their physical
CC properties. {ECO:0000269|PubMed:25115560}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced.According to EST sequences.
CC {ECO:0000305};
CC Name=1;
CC IsoId=Q6DBP4-1; Sequence=Displayed;
CC -!- DISRUPTION PHENOTYPE: Reduced inflorescence stem growth and increased
CC levels of acetate in rosette leaves. {ECO:0000269|PubMed:25115560}.
CC -!- SIMILARITY: Belongs to the pectinacetylesterase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA18629.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78944.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL022580; CAA18629.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161550; CAB78944.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84179.2; -; Genomic_DNA.
DR EMBL; CP002687; AEE84180.1; -; Genomic_DNA.
DR EMBL; BT014978; AAT70429.1; -; mRNA.
DR EMBL; BT015714; AAU45212.1; -; mRNA.
DR PIR; T05825; T05825.
DR RefSeq; NP_001319996.1; NM_001341336.1. [Q6DBP4-1]
DR RefSeq; NP_193677.2; NM_118062.7. [Q6DBP4-1]
DR AlphaFoldDB; Q6DBP4; -.
DR SMR; Q6DBP4; -.
DR STRING; 3702.AT4G19420.1; -.
DR ESTHER; arath-o65713; Pectinacetylesterase-Notum.
DR PaxDb; Q6DBP4; -.
DR PRIDE; Q6DBP4; -.
DR ProteomicsDB; 236319; -. [Q6DBP4-1]
DR EnsemblPlants; AT4G19420.1; AT4G19420.1; AT4G19420. [Q6DBP4-1]
DR EnsemblPlants; AT4G19420.2; AT4G19420.2; AT4G19420. [Q6DBP4-1]
DR GeneID; 827683; -.
DR Gramene; AT4G19420.1; AT4G19420.1; AT4G19420. [Q6DBP4-1]
DR Gramene; AT4G19420.2; AT4G19420.2; AT4G19420. [Q6DBP4-1]
DR KEGG; ath:AT4G19420; -.
DR Araport; AT4G19420; -.
DR TAIR; locus:2140436; AT4G19420.
DR eggNOG; KOG4287; Eukaryota.
DR InParanoid; Q6DBP4; -.
DR OMA; GCRDDIT; -.
DR PhylomeDB; Q6DBP4; -.
DR PRO; PR:Q6DBP4; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q6DBP4; baseline and differential.
DR Genevisible; Q6DBP4; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0052793; F:pectin acetylesterase activity; IBA:GO_Central.
DR GO; GO:0071555; P:cell wall organization; IBA:GO_Central.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR004963; PAE/NOTUM.
DR PANTHER; PTHR21562; PTHR21562; 1.
DR Pfam; PF03283; PAE; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell wall; Cell wall biogenesis/degradation;
KW Glycoprotein; Hydrolase; Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..397
FT /note="Pectin acetylesterase 8"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431773"
FT ACT_SITE 172
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6P988"
FT ACT_SITE 268
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6P988"
FT ACT_SITE 335
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q6P988"
FT CARBOHYD 73
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 353
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 397 AA; 44439 MW; DE9776B596206978 CRC64;
MFKLKQWLIY LVCSLVIMNT EGLFVNITFV RNAVAKGAVC LDGSPPAYHL DRGSGTGINS
WLIQLEGGGW CNNVTNCVSR MHTRLGSSKK MVENLAFSAI LSNKKQYNPD FYNWNRVKVR
YCDGASFTGD VEAVNPATNL HFRGARVWLA VMQELLAKGM INAENAVLSG CSAGGLASLM
HCDSFRALLP MGTKVKCLSD AGFFLNTRDV SGVQYIKTYF EDVVTLHGSA KNLPRSCTSR
LTPAMCFFPQ YVARQIRTPL FILNAAYDSW QIKNILAPRA ADPYGKWQSC QLDIKNCHPS
QIKVMQDFRL EFLSAVIGLG RSSSRGMFID SCYTHCQTET QTSWFWQDSP ILNRTTIAKA
VGDWVYDRTL FQKIDCPYPC NPTCHHRVFT PLDAPPI
