PAF15_XENTR
ID PAF15_XENTR Reviewed; 125 AA.
AC Q6AZL2;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=PCNA-associated factor {ECO:0000305};
DE AltName: Full=PCNA-associated factor of 15 kDa;
DE Short=PAF15;
DE Short=p15PAF;
DE AltName: Full=PCNA-clamp-associated factor {ECO:0000250|UniProtKB:Q15004};
GN Name=pclaf {ECO:0000250|UniProtKB:Q15004}; Synonyms=paf;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=20431018; DOI=10.1126/science.1183670;
RA Hellsten U., Harland R.M., Gilchrist M.J., Hendrix D., Jurka J.,
RA Kapitonov V., Ovcharenko I., Putnam N.H., Shu S., Taher L., Blitz I.L.,
RA Blumberg B., Dichmann D.S., Dubchak I., Amaya E., Detter J.C., Fletcher R.,
RA Gerhard D.S., Goodstein D., Graves T., Grigoriev I.V., Grimwood J.,
RA Kawashima T., Lindquist E., Lucas S.M., Mead P.E., Mitros T., Ogino H.,
RA Ohta Y., Poliakov A.V., Pollet N., Robert J., Salamov A., Sater A.K.,
RA Schmutz J., Terry A., Vize P.D., Warren W.C., Wells D., Wills A.,
RA Wilson R.K., Zimmerman L.B., Zorn A.M., Grainger R., Grammer T.,
RA Khokha M.K., Richardson P.M., Rokhsar D.S.;
RT "The genome of the Western clawed frog Xenopus tropicalis.";
RL Science 328:633-636(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: PCNA-binding protein that acts as a regulator of DNA repair
CC during DNA replication. Following DNA damage, the interaction with pcna
CC is disrupted, facilitating the interaction between monoubiquitinated
CC pcna and the translesion DNA synthesis DNA polymerase eta (polh) at
CC stalled replisomes, facilitating the bypass of replication-fork-
CC blocking lesions. Also acts as a regulator of centrosome number (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with pcna. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q15004}.
CC Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:Q15004}.
CC Note=Following DNA damage, localizes to DNA damage sites. Colocalizes
CC with centrosomes in perinuclear region. {ECO:0000250|UniProtKB:Q15004}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AAMC01085630; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC077667; AAH77667.1; -; mRNA.
DR RefSeq; NP_001005130.1; NM_001005130.2.
DR AlphaFoldDB; Q6AZL2; -.
DR SMR; Q6AZL2; -.
DR STRING; 8364.ENSXETP00000057278; -.
DR PaxDb; Q6AZL2; -.
DR DNASU; 448713; -.
DR Ensembl; ENSXETT00000057278; ENSXETP00000057278; ENSXETG00000027460.
DR GeneID; 448713; -.
DR KEGG; xtr:448713; -.
DR CTD; 9768; -.
DR Xenbase; XB-GENE-1006964; pclaf.
DR eggNOG; ENOG502S3UM; Eukaryota.
DR HOGENOM; CLU_142343_0_0_1; -.
DR InParanoid; Q6AZL2; -.
DR OMA; NRCISDS; -.
DR OrthoDB; 1508906at2759; -.
DR PhylomeDB; Q6AZL2; -.
DR TreeFam; TF333199; -.
DR Reactome; R-XTR-5656169; Termination of translesion DNA synthesis.
DR Proteomes; UP000008143; Chromosome 3.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000027460; Expressed in 2-cell stage embryo and 13 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISS:UniProtKB.
DR GO; GO:0007098; P:centrosome cycle; ISS:UniProtKB.
DR GO; GO:0006260; P:DNA replication; ISS:UniProtKB.
DR GO; GO:0051726; P:regulation of cell cycle; ISS:UniProtKB.
DR GO; GO:0009411; P:response to UV; ISS:UniProtKB.
DR GO; GO:0019985; P:translesion synthesis; ISS:UniProtKB.
DR InterPro; IPR040444; PCNA-AF.
DR InterPro; IPR031444; PCNA-AF_dom.
DR PANTHER; PTHR15679; PTHR15679; 1.
DR Pfam; PF15715; PAF; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; DNA damage; DNA repair; Nucleus; Reference proteome.
FT CHAIN 1..125
FT /note="PCNA-associated factor"
FT /id="PRO_0000420483"
FT REGION 1..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 28..39
FT /note="D-box"
FT MOTIF 68..79
FT /note="PIP-box"
FT MOTIF 85..87
FT /note="KEN box"
FT MOTIF 95..107
FT /note="Initiation motif"
FT COMPBIAS 1..18
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 29..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 125 AA; 12885 MW; 72B3D8CD184ED6C2 CRC64;
MVRTKADSAG SSASSGSYRK AVAARAPRKT FGSSSSGSNH VTSPTGKKSE SKYAGGNPVC
VRPTPTWQKG IGEFFGSPST SQPEKENRIP SDDEEAGGSG AGKAPRKSRP LPPDPSEEAA
DSDDE