ASL1B_DANRE
ID ASL1B_DANRE Reviewed; 195 AA.
AC Q90260;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Achaete-scute homolog 1b;
DE Short=Zash-1b;
GN Name=ascl1b {ECO:0000312|ZFIN:ZDB-GENE-980526-174};
GN Synonyms=ash {ECO:0000312|EMBL:AAA78899.1},
GN ashb {ECO:0000312|ZFIN:ZDB-GENE-980526-174};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAA78899.1}
RP NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RC TISSUE=Embryo {ECO:0000269|PubMed:7813774};
RX PubMed=7813774; DOI=10.1006/dbio.1994.1334;
RA Allende M.L., Weinberg E.S.;
RT "The expression pattern of two zebrafish achaete-scute homolog (ash) genes
RT is altered in the embryonic brain of the cyclops mutant.";
RL Dev. Biol. 166:509-530(1994).
RN [2] {ECO:0000312|EMBL:AAH67718.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo {ECO:0000312|EMBL:AAH67718.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15659486; DOI=10.1242/dev.01616;
RA Amoyel M., Cheng Y.-C., Jiang Y.-J., Wilkinson D.G.;
RT "Wnt1 regulates neurogenesis and mediates lateral inhibition of boundary
RT cell specification in the zebrafish hindbrain.";
RL Development 132:775-785(2005).
RN [4] {ECO:0000305}
RP TISSUE SPECIFICITY.
RX PubMed=16481349; DOI=10.1242/dev.02296;
RA Pogoda H.-M., von der Hardt S., Herzog W., Kramer C., Schwarz H.,
RA Hammerschmidt M.;
RT "The proneural gene ascl1a is required for endocrine differentiation and
RT cell survival in the zebrafish adenohypophysis.";
RL Development 133:1079-1089(2006).
CC -!- FUNCTION: Transcriptional regulator. May mediate transcription
CC activation by binding to the E box-containing promoter (By similarity).
CC Involved in neurogenesis. Involved in maintaining rhombomere boundaries
CC in the hindbrain, probably via up-regulation of delta expression. May
CC mediate transcription activation by binding to the E box-containing
CC promoter. {ECO:0000250, ECO:0000269|PubMed:15659486}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. {ECO:0000250|UniProtKB:P50553}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: In the 24 hours embryo, expressed in hindbrain
CC close to the anterior and posterior boundaries of rhombomeres 2-6 and
CC in ventral cells close to the floor plate of most rhombomeres. Also
CC expressed in the telencephalon, diencephalon, tegmentum and spinal cord
CC at sites distinct from those expressing ascl1a. Not expressed in the
CC adenohypophysis. {ECO:0000269|PubMed:15659486,
CC ECO:0000269|PubMed:16481349, ECO:0000269|PubMed:7813774}.
CC -!- DEVELOPMENTAL STAGE: First detected at 10 hours post-fertilization
CC (hpf) increasing over the next 24 hours, then decreasing between 48 and
CC 72 hours. Not detected in adult. {ECO:0000269|PubMed:7813774}.
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DR EMBL; U14590; AAA78899.1; -; mRNA.
DR EMBL; BC067718; AAH67718.1; -; mRNA.
DR PIR; I50508; I50508.
DR RefSeq; NP_571306.1; NM_131231.1.
DR AlphaFoldDB; Q90260; -.
DR SMR; Q90260; -.
DR PaxDb; Q90260; -.
DR DNASU; 30478; -.
DR Ensembl; ENSDART00000019446; ENSDARP00000022655; ENSDARG00000101628.
DR GeneID; 30478; -.
DR KEGG; dre:30478; -.
DR CTD; 30478; -.
DR ZFIN; ZDB-GENE-980526-174; ascl1b.
DR GeneTree; ENSGT00940000165300; -.
DR HOGENOM; CLU_063523_3_1_1; -.
DR InParanoid; Q90260; -.
DR OMA; CKRRLAF; -.
DR OrthoDB; 1131543at2759; -.
DR PhylomeDB; Q90260; -.
DR TreeFam; TF322889; -.
DR PRO; PR:Q90260; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 7.
DR Bgee; ENSDARG00000101628; Expressed in hindbrain and 39 other tissues.
DR GO; GO:0005634; C:nucleus; IC:ZFIN.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; NAS:ZFIN.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007420; P:brain development; IEP:ZFIN.
DR GO; GO:0043009; P:chordate embryonic development; IEP:ZFIN.
DR GO; GO:0031018; P:endocrine pancreas development; IMP:ZFIN.
DR GO; GO:0021575; P:hindbrain morphogenesis; IMP:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0050767; P:regulation of neurogenesis; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007423; P:sensory organ development; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR015660; MASH1/Ascl1a-like.
DR PANTHER; PTHR13935; PTHR13935; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Neurogenesis; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..195
FT /note="Achaete-scute homolog 1b"
FT /id="PRO_0000271236"
FT DOMAIN 66..118
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 141..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 141..162
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 195 AA; 22116 MW; EB91B514C2BB6DA7 CRC64;
MEATVVATTQ LTQDSFYQPF SESLEKQDRE CKVLKRQRSS SPELLRCKRR LTFNGLGYTI
PQQQPMAVAR RNERERNRVK QVNMGFQTLR QHVPNGAANK KMSKVETLRS AVEYIRALQQ
LLDEHDAVSA VLQCGVPSPS VSNAYSAGPE SPHSAYSSDE GSYEHLSSEE QELLDFTTWF
DRYESGASMA TKDWC
