ID   ASL1_BACSE              Reviewed;          11 AA.
AC   P83146;
DT   17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   11-DEC-2019, entry version 25.
DE   RecName: Full=Acharan sulfate lyase 1;
DE            EC=4.2.2.-;
DE   Flags: Fragment;
OS   Bacteroides stercoris.
OC   Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC   Bacteroides.
OX   NCBI_TaxID=46506 {ECO:0000305};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, ACTIVITY REGULATION, AND SUBUNIT.
RC   STRAIN=HJ-15;
RX   PubMed=11322884; DOI=10.1046/j.1432-1327.2001.02156.x;
RA   Kim B.-T., Hong S.-W., Kim W.-S., Kim Y.S., Kim D.-H.;
RT   "Purification and characterization of acharan sulfate lyases, two novel
RT   heparinases, from Bacteroides stercoris HJ-15.";
RL   Eur. J. Biochem. 268:2635-2641(2001).
CC   -!- FUNCTION: Degrades acharan sulfate and, to a lesser extent, heparin and
CC       heparan sulfate. {ECO:0000269|PubMed:11322884}.
CC   -!- ACTIVITY REGULATION: Inhibited by Cu(2+) ion, nitrogen and cobalt.
CC       Activated by reducing agents, such as DL-dithiothreitol and 2-
CC       mercaptoethanol. {ECO:0000269|PubMed:11322884}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 7.2.;
CC       Temperature dependence:
CC         Optimum temperature 45 degrees Celsius.;
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:11322884}.
CC   -!- PTM: The N-terminus is blocked. {ECO:0000269|PubMed:11322884}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   GO; GO:0008201; F:heparin binding; IDA:UniProtKB.
DR   GO; GO:0016829; F:lyase activity; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Heparin-binding; Lyase.
FT   CHAIN           <1..>11
FT                   /note="Acharan sulfate lyase 1"
FT                   /id="PRO_0000064700"
FT   NON_TER         1
FT                   /evidence="ECO:0000303|PubMed:11322884"
FT   NON_TER         11
FT                   /evidence="ECO:0000303|PubMed:11322884"
SQ   SEQUENCE   11 AA;  1395 MW;  01B2DAA241E865AB CRC64;
     NYIYSGHNYH Q