ID   PAFB_MYCTU              Reviewed;         332 AA.
AC   P9WIM1; A0A112; L0TA73; P64941; Q10705;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=Protein PafB;
DE   AltName: Full=Proteasome accessory factor B;
GN   Name=pafB; OrderedLocusNames=Rv2096c; ORFNames=MTCY49.36c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], ROLE IN RESISTANCE TO RNI, OPERON
RP   STRUCTURE, INTERACTION WITH PAFC, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=17277063; DOI=10.1128/jb.01597-06;
RA   Festa R.A., Pearce M.J., Darwin K.H.;
RT   "Characterization of the proteasome accessory factor (paf) operon in
RT   Mycobacterium tuberculosis.";
RL   J. Bacteriol. 189:3044-3050(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Part of the pafABC operon, however PafB does not seem to be
CC       involved in pupylation or substrate degradation. Appears to play at
CC       least a small role in resistance to reactive nitrogen intermediates
CC       (RNI) in vitro. {ECO:0000269|PubMed:17277063}.
CC   -!- SUBUNIT: Interacts with PafC; with which it probably forms a
CC       heterocomplex. {ECO:0000269|PubMed:17277063}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene are not severely
CC       sensitized to RNI and display no accumulation of the proteasome
CC       substrates mpa, FabD and PanB. {ECO:0000269|PubMed:17277063}.
CC   -!- SIMILARITY: Belongs to the PafB family. {ECO:0000305}.
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DR   EMBL; DQ990836; ABJ90141.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP44871.1; -; Genomic_DNA.
DR   PIR; C70768; C70768.
DR   RefSeq; NP_216612.1; NC_000962.3.
DR   RefSeq; WP_003410775.1; NZ_NVQJ01000061.1.
DR   AlphaFoldDB; P9WIM1; -.
DR   SMR; P9WIM1; -.
DR   STRING; 83332.Rv2096c; -.
DR   PaxDb; P9WIM1; -.
DR   DNASU; 888436; -.
DR   GeneID; 888436; -.
DR   KEGG; mtu:Rv2096c; -.
DR   TubercuList; Rv2096c; -.
DR   eggNOG; COG2378; Bacteria.
DR   OMA; MFERDKD; -.
DR   PhylomeDB; P9WIM1; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   InterPro; IPR026881; WYL_dom.
DR   Pfam; PF13280; WYL; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..332
FT                   /note="Protein PafB"
FT                   /id="PRO_0000103963"
SQ   SEQUENCE   332 AA;  35300 MW;  5867925C8A29214E CRC64;
     MATSKVERLV NLVIALLSTR GYITAEKIRS SVAGYSDSPS VEAFSRMFER DKNELRDLGI
     PLEVGRVSAL EPTEGYRINR DAYALSPVEL TPDEAAAVAV ATQLWESPEL ITATQGALLK
     LRAAGVDVDP LDTGAPVAIA SAAAVSGLRG SEDVLGILLS AIDSGQVVQF SHRSSRAEPY
     TVRTVEPWGV VTEKGRWYLV GHDRDRDATR VFRLSRIGAQ VTPIGPAGAT TVPAGVDLRS
     IVAQKVTEVP TGEQATVWVA EGRATALRRA GRSAGPRQLG GRDGEVIELE IRSSDRLARE
     ITGYGADAIV LQPGSLRDDV LARLRAQAGA LA