ID   PAFC_MYCTU              Reviewed;         316 AA.
AC   P9WIL9; A0A113; L0TBD1; Q10704;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=Protein PafC;
DE   AltName: Full=Proteasome accessory factor C;
GN   Name=pafC; OrderedLocusNames=Rv2095c; ORFNames=MTCY49.35c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], ROLE IN RESISTANCE TO RNI, OPERON
RP   STRUCTURE, INTERACTION WITH PAFC, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=17277063; DOI=10.1128/jb.01597-06;
RA   Festa R.A., Pearce M.J., Darwin K.H.;
RT   "Characterization of the proteasome accessory factor (paf) operon in
RT   Mycobacterium tuberculosis.";
RL   J. Bacteriol. 189:3044-3050(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Part of the pafABC operon, but PafC does not seem to be
CC       involved in pupylation or substrate degradation. Appears to play at
CC       least a small role in resistance to reactive nitrogen intermediates
CC       (RNI) in vitro. {ECO:0000269|PubMed:17277063}.
CC   -!- SUBUNIT: Interacts with PafB; with which it probably forms a
CC       heterocomplex. {ECO:0000269|PubMed:17277063}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene are not severely
CC       sensitized to RNI and display no accumulation of the proteasome
CC       substrates Mpa, FabD and PanB. {ECO:0000269|PubMed:17277063}.
CC   -!- SIMILARITY: Belongs to the PafC family. {ECO:0000305}.
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DR   EMBL; DQ990836; ABJ90142.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP44870.1; -; Genomic_DNA.
DR   PIR; B70768; B70768.
DR   RefSeq; NP_216611.1; NC_000962.3.
DR   RefSeq; WP_003410772.1; NZ_NVQJ01000061.1.
DR   AlphaFoldDB; P9WIL9; -.
DR   SMR; P9WIL9; -.
DR   STRING; 83332.Rv2095c; -.
DR   PaxDb; P9WIL9; -.
DR   DNASU; 888403; -.
DR   GeneID; 888403; -.
DR   KEGG; mtu:Rv2095c; -.
DR   TubercuList; Rv2095c; -.
DR   eggNOG; COG2378; Bacteria.
DR   OMA; LPMCGTS; -.
DR   PhylomeDB; P9WIL9; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   InterPro; IPR028349; PafC.
DR   InterPro; IPR043839; PafC_HTH.
DR   InterPro; IPR026881; WYL_dom.
DR   Pfam; PF19187; HTH_PafC; 1.
DR   Pfam; PF13280; WYL; 1.
DR   PIRSF; PIRSF016838; PafC; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..316
FT                   /note="Protein PafC"
FT                   /id="PRO_0000014122"
SQ   SEQUENCE   316 AA;  33764 MW;  88D4ED2B9F5BAD76 CRC64;
     MSALSTRLVR LLNMVPYFQA NPRITRAEAA AELGVTAKQL EEDLNQLWMC GLPGYSPGDL
     IDFEFCGDTI EVTFSAGIDR PLKLTSPEAT GLLVALRALA DIPGVVDPQA ARSAIAKIAA
     AAGAVAAVAE QAPTESPAAA AVRAAVRNSR ALTIDYYAAS HDTLTTRIVD PIRVLLIGGH
     SYLEAWSREA EGVRLFRFDR IVDAAELGEP AVPPESARQA PPDTSLFDGD LSLPSATLRV
     APSASWMLEY YPIRELRQLP DGSCEVAMTY ASEDWMTRLL LGFGSDVRVL APESLAQRVR
     DAATAALDAY QAAAPP