ID   PAG1_PIG                Reviewed;         389 AA.
AC   Q29078;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Pregnancy-associated glycoprotein 1;
DE            Short=PAG 1;
DE            Short=PAG1;
DE            EC=3.4.23.-;
DE   Flags: Precursor;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7669851; DOI=10.1095/biolreprod53.1.21;
RA   Szafranska B., Xie S., Green J., Roberts R.M.;
RT   "Porcine pregnancy-associated glycoproteins: new members of the aspartic
RT   proteinase gene family expressed in trophectoderm.";
RL   Biol. Reprod. 53:21-28(1995).
CC   -!- FUNCTION: Appears to be proteolytically inactive.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC   -!- TISSUE SPECIFICITY: Expressed throughout the chorion, with the signal
CC       localized exclusively over the trophectoderm.
CC   -!- DEVELOPMENTAL STAGE: Expression was detected at day 15, coinciding with
CC       the beginning of implantation, and continued throughout gestation.
CC   -!- SIMILARITY: Belongs to the peptidase A1 family. {ECO:0000305}.
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DR   EMBL; L34360; AAA81530.1; -; Genomic_DNA.
DR   PIR; I46616; I46616.
DR   AlphaFoldDB; Q29078; -.
DR   SMR; Q29078; -.
DR   STRING; 9823.ENSSSCP00000013930; -.
DR   MEROPS; A01.971; -.
DR   PaxDb; Q29078; -.
DR   eggNOG; KOG1339; Eukaryota.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR   Gene3D; 2.40.70.10; -; 2.
DR   InterPro; IPR001461; Aspartic_peptidase_A1.
DR   InterPro; IPR001969; Aspartic_peptidase_AS.
DR   InterPro; IPR012848; Aspartic_peptidase_N.
DR   InterPro; IPR033121; PEPTIDASE_A1.
DR   InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR   PANTHER; PTHR47966; PTHR47966; 1.
DR   Pfam; PF07966; A1_Propeptide; 1.
DR   Pfam; PF00026; Asp; 1.
DR   PRINTS; PR00792; PEPSIN.
DR   SUPFAM; SSF50630; SSF50630; 1.
DR   PROSITE; PS00141; ASP_PROTEASE; 1.
DR   PROSITE; PS51767; PEPTIDASE_A1; 1.
PE   2: Evidence at transcript level;
KW   Aspartyl protease; Disulfide bond; Glycoprotein; Hydrolase; Protease;
KW   Reference proteome; Secreted; Signal; Zymogen.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   PROPEP          16..?
FT                   /note="Activation peptide"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000026105"
FT   CHAIN           ?..389
FT                   /note="Pregnancy-associated glycoprotein 1"
FT                   /id="PRO_0000026106"
FT   DOMAIN          76..386
FT                   /note="Peptidase A1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
FT   ACT_SITE        94
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT   ACT_SITE        277
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT   CARBOHYD        79
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        130
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        348
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        107..112
FT                   /evidence="ECO:0000250"
FT   DISULFID        268..272
FT                   /evidence="ECO:0000250"
FT   DISULFID        311..345
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   389 AA;  43533 MW;  764A1D2C17A3F416 CRC64;
     MKWLVILGLV ALSECLVIIP LTKVKSIREN LREKDLLLNF LKEHPYNMIQ KFGLKGSLCS
     PKISCLRLWN YLDMVYVGNI TIGTPPQLFS VIFDTASSDL WVPSNQCHSR ACVTHRSFNP
     TLSSTFQSSN RTVKLAPHSG LVSGLLGYDT VQIGRFKSEN QAFGLSQSEP VKELENAFFD
     GVLGLGYPSL AIQGTTPVFD NLRKQGQIPE PVFALYLSTN TKKGSVLMIG GVDNNFFTGN
     LKWVPLSARN YWQITLDRIT WRGVVVGCTR GCQAILDSGS AFLLGPSRQI SSIQKIIQAR
     FIENEYQVRC CARTTLADFI FTINNVQYPV PARAYIRKGS TPRRCYSNFS GGTESLGKEE
     TWILGEVFLR LYFTVFDRGQ NRIGLRIAV