ID   PAG2_PIG                Reviewed;         420 AA.
AC   Q29079; Q29017;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Pregnancy-associated glycoprotein 2;
DE            Short=PAG 2;
DE            EC=3.4.23.-;
DE   Flags: Precursor;
GN   Name=PAG2;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7669851; DOI=10.1095/biolreprod53.1.21;
RA   Szafranska B., Xie S., Green J., Roberts R.M.;
RT   "Porcine pregnancy-associated glycoproteins: new members of the aspartic
RT   proteinase gene family expressed in trophectoderm.";
RL   Biol. Reprod. 53:21-28(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Placenta;
RX   PubMed=11553911; DOI=10.1002/mrd.1070;
RA   Szafranska B., Miura R., Ghosh D., Ezashi T., Xie S., Roberts R.M.,
RA   Green J.A.;
RT   "Gene for porcine pregnancy-associated glycoprotein 2 (poPAG2): its
RT   structural organization and analysis of its promoter.";
RL   Mol. Reprod. Dev. 60:137-146(2001).
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC   -!- TISSUE SPECIFICITY: Expressed throughout the chorion, with the signal
CC       localized exclusively over the trophectoderm.
CC   -!- DEVELOPMENTAL STAGE: Expression was detected at day 15, coinciding with
CC       the beginning of implantation, and continued throughout gestation.
CC   -!- SIMILARITY: Belongs to the peptidase A1 family. {ECO:0000305}.
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DR   EMBL; U39763; AAA92055.1; -; Genomic_DNA.
DR   EMBL; U41421; AAA92055.1; JOINED; Genomic_DNA.
DR   EMBL; U41422; AAA92055.1; JOINED; Genomic_DNA.
DR   EMBL; U39199; AAA92055.1; JOINED; Genomic_DNA.
DR   EMBL; U41423; AAA92055.1; JOINED; Genomic_DNA.
DR   EMBL; U41424; AAA92055.1; JOINED; Genomic_DNA.
DR   EMBL; U39762; AAA92055.1; JOINED; Genomic_DNA.
DR   EMBL; L34361; AAA81531.1; -; Genomic_DNA.
DR   PIR; I46617; I46617.
DR   AlphaFoldDB; Q29079; -.
DR   SMR; Q29079; -.
DR   MEROPS; A01.051; -.
DR   PRIDE; Q29079; -.
DR   InParanoid; Q29079; -.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR   Gene3D; 2.40.70.10; -; 3.
DR   InterPro; IPR001461; Aspartic_peptidase_A1.
DR   InterPro; IPR001969; Aspartic_peptidase_AS.
DR   InterPro; IPR012848; Aspartic_peptidase_N.
DR   InterPro; IPR033121; PEPTIDASE_A1.
DR   InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR   PANTHER; PTHR47966; PTHR47966; 2.
DR   Pfam; PF07966; A1_Propeptide; 1.
DR   Pfam; PF00026; Asp; 2.
DR   PRINTS; PR00792; PEPSIN.
DR   SUPFAM; SSF50630; SSF50630; 2.
DR   PROSITE; PS00141; ASP_PROTEASE; 2.
DR   PROSITE; PS51767; PEPTIDASE_A1; 1.
PE   2: Evidence at transcript level;
KW   Aspartyl protease; Disulfide bond; Glycoprotein; Hydrolase; Protease;
KW   Reference proteome; Secreted; Signal; Zymogen.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   PROPEP          16..?
FT                   /note="Activation peptide"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000026107"
FT   CHAIN           ?..420
FT                   /note="Pregnancy-associated glycoprotein 2"
FT                   /id="PRO_0000026108"
FT   DOMAIN          76..417
FT                   /note="Peptidase A1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
FT   ACT_SITE        94
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT   ACT_SITE        277
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT   CARBOHYD        56
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        79
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        107..112
FT                   /evidence="ECO:0000250"
FT   DISULFID        268..272
FT                   /evidence="ECO:0000250"
FT   DISULFID        341..376
FT                   /evidence="ECO:0000250"
FT   CONFLICT        335..367
FT                   /note="Missing (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   420 AA;  47132 MW;  094153B6C1B1FCDB CRC64;
     MKWLVILGLV ALSDCLVMIP LTKVKSVRES LREKGLLKNF LKEHPYNMIQ NLLSKNSSHV
     QKFSYQPLRN YLDMVYVGNI SIGTPPQQFS VVFDTGSSDL WVPSIYCKSK ACVTHRSFNP
     SHSSTFHDRG KSIKLEYGSG KMSGFLGQDT VRIGQLTSTG QAFGLSKEET GKAFEHAIFD
     GILGLAYPSI AIKGTTTVID NLKKQDQISE PVFAFYLSSD KEEGSVVMFG GVDKKYYKGD
     LKWVPLTQTS YWQIALDRIT CRGRVIGCPR GCQAIVDTGT SMLHGPSKAV AKIHSLIKHF
     EKEYVVPCNA RKALPDIVFT INNVDYPVPA QAYIRKYVVP CNARKALPDI VFTINNVDYP
     VPAQAYIRKN ANNNRCYSTF EDIMDTLNQR EIWILGDVFL RLYFTVYDEG QNRIGLAQAT