PAG4_SHEEP
ID PAG4_SHEEP Reviewed; 380 AA.
AC P83495; O02724;
DT 09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 19-FEB-2014, sequence version 2.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=Pregnancy-associated glycoprotein 4;
DE Short=ovPAG4;
DE EC=3.4.23.-;
DE AltName: Full=Pregnancy-associated glycoprotein 58c;
DE Short=ovPAG 58c;
DE Flags: Precursor;
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Placenta;
RX PubMed=9408244; DOI=10.1095/biolreprod57.6.1384;
RA Xie S., Green J., Bao B., Beckers J.F., Valdez K.E., Hakami L.,
RA Roberts R.M.;
RT "Multiple pregnancy-associated glycoproteins are secreted by day 100 ovine
RT placental tissue.";
RL Biol. Reprod. 57:1384-1393(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Placenta;
RX PubMed=9371757; DOI=10.1073/pnas.94.24.12809;
RA Xie S., Green J., Bixby J.B., Szafranska B., DeMartini J.C., Hecht S.,
RA Roberts R.M.;
RT "The diversity and evolutionary relationships of the pregnancy-associated
RT glycoproteins, an aspartic proteinase subfamily consisting of many
RT trophoblast-expressed genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:12809-12816(1997).
RN [3] {ECO:0000305}
RP PROTEIN SEQUENCE OF 54-76, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Fetal cotyledon {ECO:0000269|PubMed:15460157};
RX PubMed=15460157; DOI=10.1051/rnd:2004025;
RA El Amiri B., Remy B., De Sousa N.M., Beckers J.F.;
RT "Isolation and characterization of eight pregnancy-associated glycoproteins
RT present at high levels in the ovine placenta between day 60 and day 100 of
RT gestation.";
RL Reprod. Nutr. Dev. 44:169-181(2004).
RN [4]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=10819764; DOI=10.1095/biolreprod62.6.1624;
RA Green J.A., Xie S., Quan X., Bao B., Gan X., Mathialagan N., Beckers J.F.,
RA Roberts R.M.;
RT "Pregnancy-associated bovine and ovine glycoproteins exhibit spatially and
RT temporally distinct expression patterns during pregnancy.";
RL Biol. Reprod. 62:1624-1631(2000).
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space
CC {ECO:0000269|PubMed:9408244}.
CC -!- TISSUE SPECIFICITY: Trophoblast and placental tissue. Produced
CC specifically in the invasive binucleate cells of the placenta.
CC {ECO:0000269|PubMed:10819764, ECO:0000269|PubMed:15460157,
CC ECO:0000269|PubMed:9371757, ECO:0000269|PubMed:9408244}.
CC -!- DEVELOPMENTAL STAGE: Detected in the later stages of pregnancy between
CC day 60 and day 100 of gestation. {ECO:0000269|PubMed:10819764,
CC ECO:0000269|PubMed:15460157, ECO:0000269|PubMed:9408244}.
CC -!- SIMILARITY: Belongs to the peptidase A1 family. {ECO:0000305}.
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DR EMBL; U94790; AAB53225.1; -; mRNA.
DR RefSeq; NP_001009462.1; NM_001009462.1.
DR AlphaFoldDB; P83495; -.
DR SMR; P83495; -.
DR MEROPS; A01.089; -.
DR GeneID; 443536; -.
DR CTD; 337898; -.
DR OrthoDB; 1619495at2759; -.
DR Proteomes; UP000002356; Unplaced.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 2.40.70.10; -; 2.
DR InterPro; IPR001461; Aspartic_peptidase_A1.
DR InterPro; IPR001969; Aspartic_peptidase_AS.
DR InterPro; IPR012848; Aspartic_peptidase_N.
DR InterPro; IPR033121; PEPTIDASE_A1.
DR InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR PANTHER; PTHR47966; PTHR47966; 1.
DR Pfam; PF07966; A1_Propeptide; 1.
DR Pfam; PF00026; Asp; 1.
DR PRINTS; PR00792; PEPSIN.
DR SUPFAM; SSF50630; SSF50630; 1.
DR PROSITE; PS00141; ASP_PROTEASE; 2.
DR PROSITE; PS51767; PEPTIDASE_A1; 1.
PE 1: Evidence at protein level;
KW Aspartyl protease; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW Hydrolase; Protease; Reference proteome; Secreted; Signal.
FT SIGNAL 1..15
FT /evidence="ECO:0000255"
FT PROPEP 16..53
FT /note="Activation peptide"
FT /evidence="ECO:0000269|PubMed:15460157"
FT /id="PRO_0000425540"
FT CHAIN 54..380
FT /note="Pregnancy-associated glycoprotein 4"
FT /id="PRO_0000199523"
FT DOMAIN 71..377
FT /note="Peptidase A1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
FT ACT_SITE 89
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT ACT_SITE 270
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT CARBOHYD 74
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 125
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 102..107
FT /evidence="ECO:0000250"
FT DISULFID 261..265
FT /evidence="ECO:0000250"
FT DISULFID 303..337
FT /evidence="ECO:0000250"
SQ SEQUENCE 380 AA; 42762 MW; D58242542B624283 CRC64;
MKWLVLLGLV AFSECIFKIP LRRVKTMRKT LSGKNMLNDV LKEHPYRLPQ ISFRGSNLII
HPLRNIRDTF YVGNITIGTP PQEFQVLFDT GSSVLWVPSV LCNSSTCSIH VRFRHLQSST
FRTTNKTFWI TYGAGTMKGV VAHDTVRIGD LVSIDQPFGL SMAEYGFHGR RFDGVLGLNY
PRQSCCRPTP IFDKLKNQGA ISEPVFAFYL SKDEQEGSVV MFGGVDHRYY KGELNWVPLV
KADDWTIQVD RISMRREVIA CSDGCDALLD TGASFIHGPG RLIDDIQKLI GSEPRDLKHY
ISCSAVNTLP SIIFTINGIN YPVPAQAYIL KGSTGHCYTA FRAKRVRTST ESWVLGDVFL
RLYFSVFDRG NDRIGLAPAM
