ID   PAGR_BACAN              Reviewed;          99 AA.
AC   O31178;
DT   18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 138.
DE   RecName: Full=Transcriptional repressor PagR;
DE   AltName: Full=Protective antigen repressor;
GN   Name=pagR; Synonyms=tcrA;
GN   OrderedLocusNames=pXO1-109, BXA0166, GBAA_pXO1_0166;
OS   Bacillus anthracis.
OG   Plasmid pXO1.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC   STRAIN=UM44;
RX   PubMed=10419943; DOI=10.1128/jb.181.15.4485-4492.1999;
RA   Hoffmaster A.R., Koehler T.M.;
RT   "Autogenous regulation of the Bacillus anthracis pag operon.";
RL   J. Bacteriol. 181:4485-4492(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sterne;
RX   PubMed=10515943; DOI=10.1128/jb.181.20.6509-6515.1999;
RA   Okinaka R.T., Cloud K., Hampton O., Hoffmaster A.R., Hill K.K., Keim P.,
RA   Koehler T.M., Lamke G., Kumano S., Mahillon J., Manter D., Martinez Y.,
RA   Ricke D., Svensson R., Jackson P.J.;
RT   "Sequence and organization of pXO1, the large Bacillus anthracis plasmid
RT   harboring the anthrax toxin genes.";
RL   J. Bacteriol. 181:6509-6515(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Ames / isolate Florida / A2012;
RX   PubMed=12004073; DOI=10.1126/science.1071837;
RA   Read T.D., Salzberg S.L., Pop M., Shumway M.F., Umayam L., Jiang L.,
RA   Holtzapple E., Busch J.D., Smith K.L., Schupp J.M., Solomon D., Keim P.,
RA   Fraser C.M.;
RT   "Comparative genome sequencing for discovery of novel polymorphisms in
RT   Bacillus anthracis.";
RL   Science 296:2028-2033(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames ancestor;
RX   PubMed=18952800; DOI=10.1128/jb.01347-08;
RA   Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA   Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT   "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL   J. Bacteriol. 191:445-446(2009).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Carbosap, and Ferrara;
RX   PubMed=12067380; DOI=10.1046/j.1365-2672.2002.01660.x;
RA   Adone R., Pasquali P., La Rosa G., Marianelli C., Muscillo M.,
RA   Fasanella A., Francia M., Ciuchini F.;
RT   "Sequence analysis of the genes encoding for the major virulence factors of
RT   Bacillus anthracis vaccine strain 'Carbosap'.";
RL   J. Appl. Microbiol. 93:117-121(2002).
RN   [6]
RP   CHARACTERIZATION.
RX   PubMed=10475965; DOI=10.1046/j.1365-2672.1999.00887.x;
RA   Hoffmaster A.R., Koehler T.M.;
RT   "Control of virulence gene expression in Bacillus anthracis.";
RL   J. Appl. Microbiol. 87:279-281(1999).
CC   -!- FUNCTION: Represses the expression of the pagA and atxA genes.
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DR   EMBL; AF031382; AAB87063.1; -; Genomic_DNA.
DR   EMBL; AF065404; AAD32413.1; -; Genomic_DNA.
DR   EMBL; AE011190; AAM26111.1; -; Genomic_DNA.
DR   EMBL; AE017336; AAT28907.2; -; Genomic_DNA.
DR   EMBL; AJ413938; CAC93936.1; -; Genomic_DNA.
DR   EMBL; AJ413939; CAC93937.1; -; Genomic_DNA.
DR   PIR; E59104; E59104.
DR   RefSeq; NP_052805.1; NC_001496.1.
DR   RefSeq; WP_000215715.1; NZ_VTZH01000015.1.
DR   PDB; 2ZKZ; X-ray; 2.00 A; A/B/C/D=1-99.
DR   PDBsum; 2ZKZ; -.
DR   AlphaFoldDB; O31178; -.
DR   SMR; O31178; -.
DR   EnsemblBacteria; AAT28907; AAT28907; GBAA_pXO1_0166.
DR   GeneID; 45025513; -.
DR   KEGG; bar:GBAA_pXO1_0166; -.
DR   HOGENOM; CLU_097806_6_2_9; -.
DR   OMA; RAINHKL; -.
DR   EvolutionaryTrace; O31178; -.
DR   Proteomes; UP000000594; Plasmid pXO1.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   CDD; cd00090; HTH_ARSR; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011991; ArsR-like_HTH.
DR   InterPro; IPR001845; HTH_ArsR_DNA-bd_dom.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01022; HTH_5; 1.
DR   SMART; SM00418; HTH_ARSR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50987; HTH_ARSR_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Plasmid; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..99
FT                   /note="Transcriptional repressor PagR"
FT                   /id="PRO_0000160625"
FT   DOMAIN          9..99
FT                   /note="HTH arsR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   DNA_BIND        43..62
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   HELIX           14..24
FT                   /evidence="ECO:0007829|PDB:2ZKZ"
FT   HELIX           27..39
FT                   /evidence="ECO:0007829|PDB:2ZKZ"
FT   HELIX           44..51
FT                   /evidence="ECO:0007829|PDB:2ZKZ"
FT   HELIX           55..65
FT                   /evidence="ECO:0007829|PDB:2ZKZ"
FT   TURN            66..68
FT                   /evidence="ECO:0007829|PDB:2ZKZ"
FT   STRAND          71..75
FT                   /evidence="ECO:0007829|PDB:2ZKZ"
FT   STRAND          78..82
FT                   /evidence="ECO:0007829|PDB:2ZKZ"
FT   HELIX           86..95
FT                   /evidence="ECO:0007829|PDB:2ZKZ"
SQ   SEQUENCE   99 AA;  11556 MW;  0F035D5F36F7886C CRC64;
     MTVFVDHKIE YMSLEDDAEL LKTMAHPMRL KIVNELYKHK ALNVTQIIQI LKLPQSTVSQ
     HLCKMRGKVL KRNRQGLEIY YSINNPKVEG IIKLLNPIQ