ID   PAG_HORSE               Reviewed;         388 AA.
AC   Q28389;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Pregnancy-associated glycoprotein;
DE            Short=PAG;
DE            EC=3.4.23.-;
DE   Flags: Precursor;
GN   Name=PAG;
OS   Equus caballus (Horse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Placenta;
RX   PubMed=9561214; DOI=10.1007/978-1-4615-5373-1_22;
RA   Green J., Xie S., Gan X., Roberts R.M.;
RT   "An aspartic proteinase expressed in the equine placenta.";
RL   Adv. Exp. Med. Biol. 436:163-167(1998).
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC   -!- TISSUE SPECIFICITY: Trophoblast and placental tissue.
CC   -!- SIMILARITY: Belongs to the peptidase A1 family. {ECO:0000305}.
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DR   EMBL; L38511; AAC14885.1; -; mRNA.
DR   RefSeq; NP_001075412.1; NM_001081943.1.
DR   AlphaFoldDB; Q28389; -.
DR   SMR; Q28389; -.
DR   MEROPS; A01.051; -.
DR   PaxDb; Q28389; -.
DR   Ensembl; ENSECAT00000014230; ENSECAP00000011361; ENSECAG00000013027.
DR   GeneID; 100034171; -.
DR   KEGG; ecb:100034171; -.
DR   CTD; 10216630; -.
DR   GeneTree; ENSGT00940000153747; -.
DR   HOGENOM; CLU_013253_3_0_1; -.
DR   InParanoid; Q28389; -.
DR   OMA; YEPMRNY; -.
DR   OrthoDB; 1619495at2759; -.
DR   TreeFam; TF314990; -.
DR   Proteomes; UP000002281; Chromosome 12.
DR   Bgee; ENSECAG00000013027; Expressed in chorionic villus and 2 other tissues.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR   Gene3D; 2.40.70.10; -; 2.
DR   InterPro; IPR001461; Aspartic_peptidase_A1.
DR   InterPro; IPR001969; Aspartic_peptidase_AS.
DR   InterPro; IPR012848; Aspartic_peptidase_N.
DR   InterPro; IPR033121; PEPTIDASE_A1.
DR   InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR   PANTHER; PTHR47966; PTHR47966; 1.
DR   Pfam; PF07966; A1_Propeptide; 1.
DR   Pfam; PF00026; Asp; 1.
DR   PRINTS; PR00792; PEPSIN.
DR   SUPFAM; SSF50630; SSF50630; 1.
DR   PROSITE; PS00141; ASP_PROTEASE; 2.
DR   PROSITE; PS51767; PEPTIDASE_A1; 1.
PE   2: Evidence at transcript level;
KW   Aspartyl protease; Disulfide bond; Glycoprotein; Hydrolase; Protease;
KW   Reference proteome; Secreted; Signal; Zymogen.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   PROPEP          16..?
FT                   /note="Activation peptide"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000026103"
FT   CHAIN           ?..388
FT                   /note="Pregnancy-associated glycoprotein"
FT                   /id="PRO_0000026104"
FT   DOMAIN          74..385
FT                   /note="Peptidase A1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
FT   ACT_SITE        92
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT   ACT_SITE        275
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT   CARBOHYD        356
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        105..110
FT                   /evidence="ECO:0000250"
FT   DISULFID        266..270
FT                   /evidence="ECO:0000250"
FT   DISULFID        309..344
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   388 AA;  42891 MW;  37876CC59FB612C5 CRC64;
     MKWFGVLGLV TLSECLVTIP LVKIKSLREN LREKDMLKEY LEKYPFRLTH KLLHKHADSG
     VAFEPMRNYL DIAYMGIISV GTPPQEFQVI FDTGSADLWV PSIYCSSPAC SNHNTFNPLR
     SSTFVASGQP IKLIYGTGKM SGFVGYDTIK ISSLVDRNQA FGLSVEEPDK ILELATFDGI
     LGLSYPSLSV KGVTPVFDNL WNQGLLSQKL FAFYLSRKGK KGSVVMFGGV DPSYYTGELH
     WVPVSKPLYW QISMDSISIN GKVIACDGGC QAIVDTGTSL LLGPQDAVLN IQEIIQARRS
     TSGEYFIDCD AVNTLPDILF TIDGIGYPVP ANAYIQKDAA LGICFSSFEG NEDISNNSEE
     WILGDVFLRL YFTVFDREND RIGLATAV