PAI2_ARATH
ID PAI2_ARATH Reviewed; 275 AA.
AC Q42527; F4K0R4; Q9FFF7;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=N-(5'-phosphoribosyl)anthranilate isomerase 2, chloroplastic;
DE EC=5.3.1.24;
DE Flags: Precursor;
GN Name=PAI2; OrderedLocusNames=At5g05590; ORFNames=MOP10.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=7773017; DOI=10.2307/3870082;
RA Li J., Zhao J., Rose A.B., Schmidt R., Last R.L.;
RT "Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic
RT analysis of triplicate tryptophan pathway genes.";
RL Plant Cell 7:447-461(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM2).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=7890741; DOI=10.1074/jbc.270.11.6081;
RA Zhao J., Last R.L.;
RT "Immunological characterization and chloroplast localization of the
RT tryptophan biosynthetic enzymes of the flowering plant Arabidopsis
RT thaliana.";
RL J. Biol. Chem. 270:6081-6087(1995).
RN [8]
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=11346937; DOI=10.1007/s004250000452;
RA He Y., Li J.;
RT "Differential expression of triplicate phosphoribosylanthranilate isomerase
RT isogenes in the tryptophan biosynthetic pathway of Arabidopsis thaliana
RT (L.) Heynh.";
RL Planta 212:641-647(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-
CC carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate;
CC Xref=Rhea:RHEA:21540, ChEBI:CHEBI:18277, ChEBI:CHEBI:58613;
CC EC=5.3.1.24;
CC -!- PATHWAY: Amino-acid biosynthesis; L-tryptophan biosynthesis; L-
CC tryptophan from chorismate: step 3/5.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:7890741}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q42527-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q42527-2; Sequence=VSP_043742, VSP_043743, VSP_043744;
CC -!- TISSUE SPECIFICITY: Expressed in roots and shoots.
CC {ECO:0000269|PubMed:11346937}.
CC -!- INDUCTION: By silver nitrate and UV irradiation.
CC {ECO:0000269|PubMed:11346937}.
CC -!- SIMILARITY: Belongs to the TrpF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB11548.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BX829410; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; U18968; AAC49003.1; -; Genomic_DNA.
DR EMBL; AB005241; BAB11548.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED90894.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90895.1; -; Genomic_DNA.
DR EMBL; BT003070; AAO23635.1; -; mRNA.
DR EMBL; AK227265; BAE99293.1; -; mRNA.
DR EMBL; BX829410; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_196178.2; NM_120641.5. [Q42527-1]
DR RefSeq; NP_974734.1; NM_203005.2. [Q42527-2]
DR AlphaFoldDB; Q42527; -.
DR SMR; Q42527; -.
DR STRING; 3702.AT5G05590.1; -.
DR PaxDb; Q42527; -.
DR PRIDE; Q42527; -.
DR ProteomicsDB; 248744; -. [Q42527-1]
DR EnsemblPlants; AT5G05590.1; AT5G05590.1; AT5G05590. [Q42527-1]
DR EnsemblPlants; AT5G05590.2; AT5G05590.2; AT5G05590. [Q42527-2]
DR GeneID; 830442; -.
DR Gramene; AT5G05590.1; AT5G05590.1; AT5G05590. [Q42527-1]
DR Gramene; AT5G05590.2; AT5G05590.2; AT5G05590. [Q42527-2]
DR KEGG; ath:AT5G05590; -.
DR Araport; AT5G05590; -.
DR TAIR; locus:2169687; AT5G05590.
DR eggNOG; KOG4202; Eukaryota.
DR HOGENOM; CLU_076364_0_0_1; -.
DR OMA; CEIMEIC; -.
DR OrthoDB; 1536911at2759; -.
DR PhylomeDB; Q42527; -.
DR BioCyc; ARA:AT5G05590-MON; -.
DR UniPathway; UPA00035; UER00042.
DR PRO; PR:Q42527; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q42527; baseline and differential.
DR Genevisible; Q42527; AT.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0004640; F:phosphoribosylanthranilate isomerase activity; ISS:TAIR.
DR GO; GO:0000162; P:tryptophan biosynthetic process; IMP:TAIR.
DR CDD; cd00405; PRAI; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR HAMAP; MF_00135; PRAI; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR001240; PRAI_dom.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR InterPro; IPR044643; TrpF_fam.
DR PANTHER; PTHR42894; PTHR42894; 1.
DR Pfam; PF00697; PRAI; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Amino-acid biosynthesis;
KW Aromatic amino acid biosynthesis; Chloroplast; Isomerase; Plastid;
KW Reference proteome; Transit peptide; Tryptophan biosynthesis.
FT TRANSIT 1..32
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 33..275
FT /note="N-(5'-phosphoribosyl)anthranilate isomerase 2,
FT chloroplastic"
FT /id="PRO_0000417454"
FT VAR_SEQ 146
FT /note="Q -> QVLYILIDFDFALTKVIFSTLLTVDSYGFLQ (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_043742"
FT VAR_SEQ 201..225
FT /note="SGHGFNWAQFKLPSVRSRNGWLLAG -> RYQEQLFNFFRIVMYSLEEKFKQ
FT SL (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_043743"
FT VAR_SEQ 226..275
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_043744"
SQ SEQUENCE 275 AA; 29619 MW; FF55DC899925F2C7 CRC64;
MSTGISTDLH VHFGALNFSK TYKSGLSNRT VSFSRVGYAQ NRKLSCSVSN TENVAPKDDE
RGKDRPLVKM CGITSARDAA MAVEAGADFI GMIIWPHSKR SISLSVAKDI SKVAREGGAK
PVGVFVEDDD NTILRAADSS DLELVQLHGN GSRAAFSRLV RKRRVIYVLN ANQDGKLLNE
VPEEDCHLAD WILVDSATGG SGHGFNWAQF KLPSVRSRNG WLLAGGINPT NVSEALSILQ
PDGIDVSSGI CGTDGIQKDK SKISSFITAV RSVHY
