PAI3_ARATH
ID PAI3_ARATH Reviewed; 244 AA.
AC Q8LPI9; Q42528; Q8VY51;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic;
DE EC=5.3.1.24;
DE Flags: Precursor;
GN Name=PAI3; OrderedLocusNames=At1g29410; ORFNames=F15D2.31;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=7773017; DOI=10.2307/3870082;
RA Li J., Zhao J., Rose A.B., Schmidt R., Last R.L.;
RT "Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic
RT analysis of triplicate tryptophan pathway genes.";
RL Plant Cell 7:447-461(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-
CC carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate;
CC Xref=Rhea:RHEA:21540, ChEBI:CHEBI:18277, ChEBI:CHEBI:58613;
CC EC=5.3.1.24;
CC -!- PATHWAY: Amino-acid biosynthesis; L-tryptophan biosynthesis; L-
CC tryptophan from chorismate: step 3/5.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8LPI9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8LPI9-2; Sequence=VSP_043745, VSP_043746;
CC -!- SIMILARITY: Belongs to the TrpF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC49004.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U18969; AAC49004.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC068667; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE31084.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31085.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59934.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59936.1; -; Genomic_DNA.
DR EMBL; AY072443; AAL62435.1; -; mRNA.
DR EMBL; AY099722; AAM20573.1; -; mRNA.
DR EMBL; BT000293; AAN15612.1; -; mRNA.
DR RefSeq; NP_001322252.1; NM_001332855.1. [Q8LPI9-2]
DR RefSeq; NP_001322254.1; NM_001332853.1. [Q8LPI9-1]
DR RefSeq; NP_174234.3; NM_102681.4. [Q8LPI9-1]
DR RefSeq; NP_850954.1; NM_180623.2. [Q8LPI9-2]
DR AlphaFoldDB; Q8LPI9; -.
DR SMR; Q8LPI9; -.
DR STRING; 3702.AT1G29410.2; -.
DR PaxDb; Q8LPI9; -.
DR PRIDE; Q8LPI9; -.
DR ProteomicsDB; 248633; -. [Q8LPI9-1]
DR EnsemblPlants; AT1G29410.1; AT1G29410.1; AT1G29410. [Q8LPI9-2]
DR EnsemblPlants; AT1G29410.2; AT1G29410.2; AT1G29410. [Q8LPI9-1]
DR EnsemblPlants; AT1G29410.4; AT1G29410.4; AT1G29410. [Q8LPI9-1]
DR EnsemblPlants; AT1G29410.6; AT1G29410.6; AT1G29410. [Q8LPI9-2]
DR GeneID; 839817; -.
DR Gramene; AT1G29410.1; AT1G29410.1; AT1G29410. [Q8LPI9-2]
DR Gramene; AT1G29410.2; AT1G29410.2; AT1G29410. [Q8LPI9-1]
DR Gramene; AT1G29410.4; AT1G29410.4; AT1G29410. [Q8LPI9-1]
DR Gramene; AT1G29410.6; AT1G29410.6; AT1G29410. [Q8LPI9-2]
DR KEGG; ath:AT1G29410; -.
DR Araport; AT1G29410; -.
DR TAIR; locus:2013588; AT1G29410.
DR eggNOG; KOG4202; Eukaryota.
DR OrthoDB; 1536911at2759; -.
DR PhylomeDB; Q8LPI9; -.
DR BioCyc; ARA:AT1G29410-MON; -.
DR UniPathway; UPA00035; UER00042.
DR PRO; PR:Q8LPI9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8LPI9; baseline and differential.
DR Genevisible; Q8LPI9; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0004640; F:phosphoribosylanthranilate isomerase activity; ISS:TAIR.
DR GO; GO:0009411; P:response to UV; IEP:TAIR.
DR GO; GO:0000162; P:tryptophan biosynthetic process; IBA:GO_Central.
DR CDD; cd00405; PRAI; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR HAMAP; MF_00135; PRAI; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR001240; PRAI_dom.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR InterPro; IPR044643; TrpF_fam.
DR PANTHER; PTHR42894; PTHR42894; 1.
DR Pfam; PF00697; PRAI; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Amino-acid biosynthesis;
KW Aromatic amino acid biosynthesis; Chloroplast; Isomerase; Plastid;
KW Reference proteome; Transit peptide; Tryptophan biosynthesis.
FT TRANSIT 1..32
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 33..244
FT /note="N-(5'-phosphoribosyl)anthranilate isomerase 3,
FT chloroplastic"
FT /id="PRO_0000417455"
FT VAR_SEQ 202..213
FT /note="YLDQLLSFFALS -> FNHNNFLLWKRI (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_043745"
FT VAR_SEQ 214..244
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_043746"
SQ SEQUENCE 244 AA; 26525 MW; 451E286CCF6E3B40 CRC64;
MSTGISSDLH LHPRALNFSK TSKSGLSNRK VSFSSVGYAQ NRKLSCSVSS TENVAPKDDD
RGKDRPLVKM CGITSARDAA MAVEAGADFI GMIIWPHSKR SISLSVAKDI SQVAREGGAK
PVGVFVEDDE NTILRAADSS DLELVQLHGN SSRAAFSRLV RERKVIYVLN ANEDGKLLNV
VPEEDGHLAD WILVDSATGG RYLDQLLSFF ALSHCNVFLR GTSYTITLVH ETVCLSQVTE
ISRV
