PAIN_DROME
ID PAIN_DROME Reviewed; 913 AA.
AC Q9W0Y6;
DT 21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Transient receptor potential cation channel protein painless;
GN Name=pain; ORFNames=CG15860;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12705873; DOI=10.1016/s0092-8674(03)00272-1;
RA Tracey W.D. Jr., Wilson R.I., Laurent G., Benzer S.;
RT "painless, a Drosophila gene essential for nociception.";
RL Cell 113:261-273(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Receptor-activated non-selective cation channel involved in
CC detection of pain sensation due to high temperature. Involved in heat
CC nociception by being activated by noxious temperature of 38 degrees
CC Celsius. {ECO:0000269|PubMed:12705873}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Present in multidendritic neurons, chordotonal
CC neurons, a subset of cells in the central nervous system and a subset
CC of sensory neurons in the antennal-maxillary complex. Not detected in
CC gonads and dorsal vessels (at protein level). Expressed in peripheral
CC neurons that extend multiple branched dendrites beneath the larval
CC epidermis, similar to vertebrate pain receptors.
CC {ECO:0000269|PubMed:12705873}.
CC -!- DEVELOPMENTAL STAGE: Expressed from stage 13 of embryonic development
CC in a small number of cells in the central nervous system and in a
CC subset of neurons of the peripheral nervous system. Such cells may
CC include sensory precursors of multidendritic (md) neurons. At embryonic
CC stage 16, prior to when dendritic process are elaborated, it appears to
CC be expressed in multidendritic neurons in a polarized manner. At stage
CC 17 of development, when the md neurons first initiate dendritogenesis,
CC it is localized to branched projections initiating from clusters of
CC multidendritic neurons.
CC -!- SIMILARITY: Belongs to the transient receptor (TC 1.A.4) family.
CC {ECO:0000305}.
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DR EMBL; AY268106; AAP03646.1; -; mRNA.
DR EMBL; AE013599; AAF47293.1; -; Genomic_DNA.
DR EMBL; BT011483; AAR99141.1; -; mRNA.
DR RefSeq; NP_611979.1; NM_138135.2.
DR AlphaFoldDB; Q9W0Y6; -.
DR SMR; Q9W0Y6; -.
DR BioGRID; 63553; 2.
DR IntAct; Q9W0Y6; 1.
DR STRING; 7227.FBpp0072323; -.
DR SwissPalm; Q9W0Y6; -.
DR PaxDb; Q9W0Y6; -.
DR PRIDE; Q9W0Y6; -.
DR DNASU; 37985; -.
DR EnsemblMetazoa; FBtr0072417; FBpp0072323; FBgn0060296.
DR GeneID; 37985; -.
DR KEGG; dme:Dmel_CG15860; -.
DR UCSC; CG15860-RA; d. melanogaster.
DR CTD; 37985; -.
DR FlyBase; FBgn0060296; pain.
DR VEuPathDB; VectorBase:FBgn0060296; -.
DR eggNOG; KOG0510; Eukaryota.
DR GeneTree; ENSGT00940000170332; -.
DR InParanoid; Q9W0Y6; -.
DR OMA; HTSIYEK; -.
DR OrthoDB; 246352at2759; -.
DR PhylomeDB; Q9W0Y6; -.
DR BioGRID-ORCS; 37985; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37985; -.
DR PRO; PR:Q9W0Y6; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0060296; Expressed in oviduct (Drosophila) and 43 other tissues.
DR ExpressionAtlas; Q9W0Y6; baseline and differential.
DR Genevisible; Q9W0Y6; DM.
DR GO; GO:0034704; C:calcium channel complex; IDA:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; NAS:FlyBase.
DR GO; GO:0005886; C:plasma membrane; HDA:FlyBase.
DR GO; GO:0005227; F:calcium activated cation channel activity; IDA:FlyBase.
DR GO; GO:0005262; F:calcium channel activity; IDA:FlyBase.
DR GO; GO:0005216; F:ion channel activity; TAS:FlyBase.
DR GO; GO:0048266; P:behavioral response to pain; IMP:FlyBase.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IDA:FlyBase.
DR GO; GO:0006816; P:calcium ion transport; ISS:FlyBase.
DR GO; GO:0007620; P:copulation; IMP:FlyBase.
DR GO; GO:0050974; P:detection of mechanical stimulus involved in sensory perception; TAS:FlyBase.
DR GO; GO:0050966; P:detection of mechanical stimulus involved in sensory perception of pain; IMP:UniProtKB.
DR GO; GO:0050965; P:detection of temperature stimulus involved in sensory perception of pain; IMP:UniProtKB.
DR GO; GO:0050960; P:detection of temperature stimulus involved in thermoception; IDA:FlyBase.
DR GO; GO:0007631; P:feeding behavior; IMP:FlyBase.
DR GO; GO:0035179; P:larval turning behavior; IMP:FlyBase.
DR GO; GO:0008049; P:male courtship behavior; IMP:FlyBase.
DR GO; GO:0007638; P:mechanosensory behavior; IMP:FlyBase.
DR GO; GO:0048060; P:negative gravitaxis; IMP:FlyBase.
DR GO; GO:0042048; P:olfactory behavior; IMP:FlyBase.
DR GO; GO:0045924; P:regulation of female receptivity; IMP:FlyBase.
DR GO; GO:0008016; P:regulation of heart contraction; IMP:FlyBase.
DR GO; GO:0009408; P:response to heat; IMP:FlyBase.
DR GO; GO:0009612; P:response to mechanical stimulus; NAS:FlyBase.
DR GO; GO:0019233; P:sensory perception of pain; IMP:FlyBase.
DR Gene3D; 1.25.40.20; -; 2.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR005821; Ion_trans_dom.
DR Pfam; PF00520; Ion_trans; 1.
DR SMART; SM00248; ANK; 4.
DR SUPFAM; SSF48403; SSF48403; 2.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
PE 1: Evidence at protein level;
KW ANK repeat; Ion channel; Ion transport; Membrane; Reference proteome;
KW Repeat; Sensory transduction; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..913
FT /note="Transient receptor potential cation channel protein
FT painless"
FT /id="PRO_0000215374"
FT TOPO_DOM 1..490
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 491..511
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 512..523
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 524..544
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 545..555
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 556..576
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 577..586
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 587..607
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 608..628
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 629..649
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 650..708
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 709..729
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 730..913
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 154..189
FT /note="ANK 1"
FT REPEAT 260..289
FT /note="ANK 2"
FT REPEAT 368..397
FT /note="ANK 3"
FT REGION 656..675
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 913 AA; 103507 MW; D89CC6082E1A03C5 CRC64;
MDFNNCGFID PQAQLAGALA KQDIRQFVAA LDSGALADLQ DDRHTSIYEK ALSTPGCRDF
IEACIDHGSQ VNYINKKLDK AAISYAADSR DPGNLAALLK YRPGNKVQVD RKYGQLTPLN
SLAKNLTDEN APDVYSCMQL LLDYGASPNI VDQGEFTPLH HVLRKSKVKA GKKELIQLFL
DHPELDIDSY RNGEVRRLLQ AQFPELKLPE ERHTGPEIDI QTLQRTLRDG DETLFEQQFA
EYLQNLKGGA DNQLNAHQEE YFGLLQESIK RGRQRAFDVI LSTGMDINSR PGRANEANLV
ETAVIYGNWQ ALERLLKEPN LRLTPDSKLL NAVIGRLDEP PYDGSSHQRC FELLINSDRV
DINEADSGRL VPLFFAVKYR NTSAMQKLLK NGAYIGSKSA FGTLPIKDMP PEVLEEHFDS
CITTNGERPG DQNFEIIIDY KNLMRQERDS GLNQLQDEMA PIAFIAESKE MRHLLQHPLI
SSFLFLKWHR LSVIFYLNFL IYSLFTASII TYTLLKFHES DQRALTAFFG LLSWLGISYL
ILRECIQWIM SPVRYFWSIT NIMEVALITL SIFTCMESSF DKETQRVLAV FTILLVSMEF
CLLVGSLPVL SISTHMLMLR EVSNSFLKSF TLYSIFVLTF SLCFYILFGK SVEEDQSKSA
TPCPPLGKKE GKDEEQGFNT FTKPIEAVIK TIVMLTGEFD AGSIQFTSIY TYLIFLLFVI
FMTIVLFNLL NGLAVSDTQV IKAQAELNGA ICRTNVLSRY EQVLTGHGRA GFLLGNHLFR
SICQRLMNIY PNYLSLRQIS VLPNDGNKVL IPMSDPFEMR TLKKASFQQL PLSAAVPQKK
LLDPPLRLLP CCCSLLTGKC SQMSGRVVKR ALEVIDQKNA AEQRRKQEQI NDSRLKLIEY
KLEQLIQLVQ DRK
