PAIR2_ORYSJ
ID PAIR2_ORYSJ Reviewed; 610 AA.
AC Q76CY8; Q0J0K5;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Meiosis-specific protein PAIR2 {ECO:0000305};
DE AltName: Full=OsASY1 {ECO:0000303|PubMed:16410547};
DE AltName: Full=Protein HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS 2 {ECO:0000303|PubMed:14758540};
GN Name=PAIR2 {ECO:0000303|PubMed:14758540};
GN Synonyms=ASY1 {ECO:0000303|PubMed:16410547};
GN OrderedLocusNames=Os09g0506800 {ECO:0000312|EMBL:BAT08867.1},
GN LOC_Os09g32930 {ECO:0000305};
GN ORFNames=OSNPB_090506800 {ECO:0000312|EMBL:BAT08867.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=14758540; DOI=10.1007/s00438-003-0934-z;
RA Nonomura K.I., Nakano M., Murata K., Miyoshi K., Eiguchi M., Miyao A.,
RA Hirochika H., Kurata N.;
RT "An insertional mutation in the rice PAIR2 gene, the ortholog of
RT Arabidopsis ASY1, results in a defect in homologous chromosome pairing
RT during meiosis.";
RL Mol. Genet. Genomics 271:121-129(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=16410547; DOI=10.1242/jcs.02736;
RA Nonomura K., Nakano M., Eiguchi M., Suzuki T., Kurata N.;
RT "PAIR2 is essential for homologous chromosome synapsis in rice meiosis I.";
RL J. Cell Sci. 119:217-225(2006).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=21119003; DOI=10.1091/mbc.e10-08-0667;
RA Wang K., Wang M., Tang D., Shen Y., Qin B., Li M., Cheng Z.;
RT "PAIR3, an axis-associated protein, is essential for the recruitment of
RT recombination elements onto meiotic chromosomes in rice.";
RL Mol. Biol. Cell 22:12-19(2011).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=27436711; DOI=10.1105/tpc.16.00108;
RA He Y., Wang C., Higgins J.D., Yu J., Zong J., Lu P., Zhang D., Liang W.;
RT "MEIOTIC F-BOX is essential for male meiotic DNA double-strand break repair
RT in rice.";
RL Plant Cell 28:1879-1893(2016).
CC -!- FUNCTION: Essential factor for meiotic homologous chromosome pairing
CC (synapsis) (PubMed:14758540). Plays an essential role in promoting
CC homologous chromosome synapsis. Does not seem to play a role in
CC chromosomal axial element formation, sister chromatid cohesion at
CC centromeres or kinetochore assembly in meiosis I (PubMed:16410547).
CC {ECO:0000269|PubMed:14758540, ECO:0000269|PubMed:16410547}.
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:16410547,
CC ECO:0000269|PubMed:21119003, ECO:0000269|PubMed:27436711}. Chromosome,
CC centromere {ECO:0000269|PubMed:16410547}. Nucleus
CC {ECO:0000269|PubMed:21119003}. Note=During interphase-early leptotene,
CC distributed as numerous punctuate foci throughout the chromatin.
CC Associates with chromosomal axes at leptotene and zygotene
CC (PubMed:16410547, PubMed:21119003). Associates with chromosomal axes at
CC zygotene (PubMed:27436711). Removed from the axes of arm regions when
CC homologous chromosomes have been synapsed (PubMed:16410547,
CC PubMed:21119003). Localizes to centromeres until diakinesis
CC (PubMed:16410547). {ECO:0000269|PubMed:16410547,
CC ECO:0000269|PubMed:21119003, ECO:0000269|PubMed:27436711}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q76CY8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q76CY8-2; Sequence=VSP_058720;
CC -!- TISSUE SPECIFICITY: Expressed in anthers and ovules.
CC {ECO:0000269|PubMed:14758540}.
CC -!- DISRUPTION PHENOTYPE: Failure of the pairing of homologous chromosomes
CC during meiosis (asynapsis or non-homologous synapsis) in both male and
CC female gametophytes. {ECO:0000269|PubMed:14758540}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
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DR EMBL; AB109238; BAD00095.1; -; mRNA.
DR EMBL; AC108753; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP008215; BAF25524.1; -; Genomic_DNA.
DR EMBL; AP014965; BAT08867.1; -; Genomic_DNA.
DR EMBL; AP014965; BAT08868.1; -; Genomic_DNA.
DR EMBL; AK064028; BAG88972.1; -; mRNA.
DR RefSeq; XP_015612272.1; XM_015756786.1. [Q76CY8-1]
DR RefSeq; XP_015612273.1; XM_015756787.1. [Q76CY8-2]
DR AlphaFoldDB; Q76CY8; -.
DR SMR; Q76CY8; -.
DR STRING; 4530.OS09T0506800-02; -.
DR PaxDb; Q76CY8; -.
DR PRIDE; Q76CY8; -.
DR EnsemblPlants; Os09t0506800-01; Os09t0506800-01; Os09g0506800. [Q76CY8-2]
DR EnsemblPlants; Os09t0506800-02; Os09t0506800-02; Os09g0506800. [Q76CY8-1]
DR GeneID; 4347514; -.
DR Gramene; Os09t0506800-01; Os09t0506800-01; Os09g0506800. [Q76CY8-2]
DR Gramene; Os09t0506800-02; Os09t0506800-02; Os09g0506800. [Q76CY8-1]
DR KEGG; osa:4347514; -.
DR eggNOG; KOG4652; Eukaryota.
DR OMA; GDLMYMK; -.
DR OrthoDB; 1038689at2759; -.
DR Proteomes; UP000000763; Chromosome 9.
DR Proteomes; UP000059680; Chromosome 9.
DR GO; GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005764; C:lysosome; IBA:GO_Central.
DR GO; GO:0000228; C:nuclear chromosome; IDA:Gramene.
DR GO; GO:0005634; C:nucleus; IDA:Gramene.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0007129; P:homologous chromosome pairing at meiosis; IDA:Gramene.
DR GO; GO:0009554; P:megasporogenesis; IDA:Gramene.
DR GO; GO:0009556; P:microsporogenesis; IDA:Gramene.
DR GO; GO:0051603; P:proteolysis involved in protein catabolic process; IBA:GO_Central.
DR Gene3D; 3.30.900.10; -; 1.
DR InterPro; IPR003511; HORMA_dom.
DR InterPro; IPR036570; HORMA_dom_sf.
DR Pfam; PF02301; HORMA; 1.
DR SUPFAM; SSF56019; SSF56019; 1.
DR PROSITE; PS50815; HORMA; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Centromere; Chromosome; Meiosis; Nucleus;
KW Reference proteome.
FT CHAIN 1..610
FT /note="Meiosis-specific protein PAIR2"
FT /id="PRO_0000438699"
FT DOMAIN 15..229
FT /note="HORMA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00109"
FT REGION 236..297
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 477..507
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 520..551
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 482..497
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 551
FT /note="Missing (in isoform 2)"
FT /id="VSP_058720"
SQ SEQUENCE 610 AA; 68803 MW; D66F92FC9FC57D74 CRC64;
MVMAQKTKEA EITEQDSLLL TRNLLRIAIY NISYIRGLFP EKYFNDKSVP ALEMKIKKLM
PMDTESRRLI DWMEKGVYDA LQKKYLKTLL FCICEKEEGP MIEEYAFSFS YPNTSGDEVA
MNLSRTGSKK NSATFKSNAA EVTPDQMRSS ACKMIRTLVS LMRTLDQMPE ERTILMKLLY
YDDVTPEDYE PPFFKCCADN EAINIWNKNP LKMEVGNVNS KHLVLALKVK SVLDPCDDNN
VNSEDDNMSL DNESDQDNDF SDTEVRPSEA ERYIVAPNDG TCKGQNGTIS EDDTQDPVHE
EELTAQVREW ICSRDTESLE VSDVLVNFPD ISMEMVEDIM ERLLKDGLLS RAKKDSYSVN
KIADPTTPHI KKEVIMQNVS PTEGTKNSNG DLMYMKALYH ALPMDYVSVG KLHGKLDGEA
SQNMVRKLIE KMVQDGYVKN SANRRLGKAV IHSEVTNRKL LEIKKILEVD IAEQMAIDTN
AEPGEPERKD HLSGHEMRDG STMGCLQSVG SDLTRTRELP EPQQNVSMQS GQEASTVDKD
PSRTPTSVRE QASVCSLESG VLGQKVRKSL AGAGGTQCSQ DKRFRKASTV KEPILQYVKR
QKSQVQVQVQ
