ID   PAKSY_ORYSJ             Reviewed;         759 AA.
AC   H2KWF1; A0A0P0XZQ3; B7EC17; C7J910; Q53NJ6; Q53NK1;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2012, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Parkeol synthase;
DE            EC=5.4.99.47;
GN   OrderedLocusNames=Os11g0189600, LOC_Os11g08569; ORFNames=OsJ_33246;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG   The rice chromosomes 11 and 12 sequencing consortia;
RT   "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT   genes and recent gene duplications.";
RL   BMC Biol. 3:20-20(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [7]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=21526825; DOI=10.1021/ol200777d;
RA   Ito R., Mori K., Hashimoto I., Nakano C., Sato T., Hoshino T.;
RT   "Triterpene cyclases from Oryza sativa L.: cycloartenol, parkeol and
RT   achilleol B synthases.";
RL   Org. Lett. 13:2678-2681(2011).
CC   -!- FUNCTION: Specifically mediates the conversion of oxidosqualene ((3S)-
CC       2,3-epoxy-2,3-dihydrosqualene) to parkeol.
CC       {ECO:0000269|PubMed:21526825}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = parkeol; Xref=Rhea:RHEA:31851,
CC         ChEBI:CHEBI:15441, ChEBI:CHEBI:63460; EC=5.4.99.47;
CC         Evidence={ECO:0000269|PubMed:21526825};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAX95229.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes.; Evidence={ECO:0000305};
CC       Sequence=AAX95231.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes.; Evidence={ECO:0000305};
CC       Sequence=ABG22398.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAG89914.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=BAH95124.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=EAZ17703.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC134046; AAX95229.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AC134046; AAX95231.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; DP000010; ABG22398.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; DP000010; ABG22399.1; -; Genomic_DNA.
DR   EMBL; AP008217; BAH95124.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014967; BAT13009.1; -; Genomic_DNA.
DR   EMBL; CM000148; EAZ17703.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AK066327; BAG89914.1; ALT_INIT; mRNA.
DR   RefSeq; XP_015617632.1; XM_015762146.1.
DR   RefSeq; XP_015617633.1; XM_015762147.1.
DR   AlphaFoldDB; H2KWF1; -.
DR   SMR; H2KWF1; -.
DR   STRING; 4530.OS11T0189600-01; -.
DR   PaxDb; H2KWF1; -.
DR   PRIDE; H2KWF1; -.
DR   EnsemblPlants; Os11t0189600-01; Os11t0189600-01; Os11g0189600.
DR   GeneID; 9268610; -.
DR   Gramene; Os11t0189600-01; Os11t0189600-01; Os11g0189600.
DR   KEGG; osa:9268610; -.
DR   eggNOG; KOG0497; Eukaryota.
DR   InParanoid; H2KWF1; -.
DR   OMA; TWLEHIN; -.
DR   OrthoDB; 365003at2759; -.
DR   BioCyc; MetaCyc:MON-17969; -.
DR   BRENDA; 5.4.99.47; 8948.
DR   BRENDA; 5.4.99.B41; 8948.
DR   Proteomes; UP000000763; Chromosome 11.
DR   Proteomes; UP000007752; Chromosome 11.
DR   Proteomes; UP000059680; Chromosome 11.
DR   ExpressionAtlas; H2KWF1; baseline and differential.
DR   Genevisible; H2KWF1; OS.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0016866; F:intramolecular transferase activity; IEA:InterPro.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Membrane; Reference proteome; Repeat; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..759
FT                   /note="Parkeol synthase"
FT                   /id="PRO_0000418965"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REPEAT          147..188
FT                   /note="PFTB 1"
FT   REPEAT          512..557
FT                   /note="PFTB 2"
FT   REPEAT          589..629
FT                   /note="PFTB 3"
FT   REPEAT          638..689
FT                   /note="PFTB 4"
FT   ACT_SITE        483
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   759 AA;  86127 MW;  98EFB2E0088C5675 CRC64;
     MWRLKVSEGG SPWLRSVNNL LGRQVWEFDP DLGTPEERAD VEKARREFAE HRFERKHSSD
     LLMRMQFAKE NCQKLDLLAV KRGEHEDVMG EAVWSSLKRA ISRVCNLQAH DGHWPGDYAG
     LMFFLPGLII TLHVSGVLNT VLSSEHQKEM RRYIYNHQNE DGGWGLHIEG HSTMLGSSLN
     YVALRLLGEG PNGGDGCIEN GRNWILDHGG ATFTTSWGKF WLSVLGVFDW SGNNPVPPEL
     LLLPYQLPFH PGRMSSYIRM VFIPMSYIYG KRFVGPVTPV VLELRSELYN DPYDEIDWNK
     ARTQCAKEDM YYPRSSKLDM FWSFLHKFIE PVLLRWPGRK LREKALATSM RNVHYEDECT
     RYICFGGVPK ALNILACWIE DPSSEAFKCH IARVYDYLWI AEDGMKMQIY DGSQVWDAGL
     TVEALVATDL VKELGPTLKR AHSFLKNSQL LDNCPRDFNR WYRHISKGGW TFTTADDGWQ
     VSDCTATALK ACLLLSRISP EIVGEPLEID AQYDAVNCLM SLMNDNGGFS AFELVRSNTW
     LEHINPTEAF GRVMIEYPYV ECTSSSIQCL ALFKKLHPGH RKEEVENCIS KGANFIESSQ
     RSDGSWYGSW GICFTYATWF AVTGLVSAGR TLGNSATVRK ACDFLLSKQL PSGGWGESYL
     SCHDEVYTNL KGNRPHGTHT AWAMIALIDA GQAERDPVPL HRAAKALLNL QLEDGEFPQQ
     EIVGVFLQTA MISYSQYRNI FPIMALTGYR RRVLLAGNI