ID   PAL1F_EUGGR             Reviewed;        1019 AA.
AC   P84738; Q2P9M9;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 2.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Photoactivated adenylate cyclase subunit alpha-like protein 1224-5/1F;
GN   Name=pacA {ECO:0000312|EMBL:CAJ57395.1};
OS   Euglena gracilis.
OC   Eukaryota; Discoba; Euglenozoa; Euglenida; Spirocuta; Euglenophyceae;
OC   Euglenales; Euglenaceae; Euglena.
OX   NCBI_TaxID=3039;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:CAJ57395.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RC   STRAIN=SAG 1224-5/1F;
RX   PubMed=16121285; DOI=10.1039/b502002f;
RA   Ntefidou M., Haeder D.-P.;
RT   "Photoactivated adenylyl cyclase (PAC) genes in the flagellate Euglena
RT   gracilis mutant strains.";
RL   Photochem. Photobiol. Sci. 4:732-739(2005).
CC   -!- SUBUNIT: Heterotetramer of two alpha and two beta subunits.
CC       {ECO:0000250|UniProtKB:Q8S9F2}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC       {ECO:0000269|PubMed:16121285}. Note=Paraxonemal body. And paraxonemal
CC       bodies (PABs).
CC   -!- MISCELLANEOUS: The 1224-5/1F strain is deficient in phototaxis. It is
CC       not known if this is due to defective adenylate cyclase activity or
CC       defective BLUF domains in this protein. {ECO:0000269|PubMed:16121285}.
CC   -!- SIMILARITY: Belongs to the adenylyl cyclase class-4/guanylyl cyclase
CC       family. {ECO:0000255|PROSITE-ProRule:PRU00099}.
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DR   EMBL; AM181336; CAJ57395.1; -; mRNA.
DR   AlphaFoldDB; P84738; -.
DR   SMR; P84738; -.
DR   GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR   GO; GO:0009882; F:blue light photoreceptor activity; IEA:InterPro.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0009190; P:cyclic nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR   CDD; cd07302; CHD; 2.
DR   Gene3D; 3.30.70.1230; -; 2.
DR   InterPro; IPR001054; A/G_cyclase.
DR   InterPro; IPR036046; Acylphosphatase-like_dom_sf.
DR   InterPro; IPR007024; BLUF_domain.
DR   InterPro; IPR029787; Nucleotide_cyclase.
DR   Pfam; PF04940; BLUF; 2.
DR   Pfam; PF00211; Guanylate_cyc; 2.
DR   SMART; SM01034; BLUF; 2.
DR   SUPFAM; SSF54975; SSF54975; 2.
DR   SUPFAM; SSF55073; SSF55073; 2.
DR   PROSITE; PS50925; BLUF; 2.
DR   PROSITE; PS50125; GUANYLATE_CYCLASE_2; 1.
PE   2: Evidence at transcript level;
KW   Cell projection; Cilium; Flagellum; Repeat.
FT   CHAIN           1..1019
FT                   /note="Photoactivated adenylate cyclase subunit alpha-like
FT                   protein 1224-5/1F"
FT                   /id="PRO_0000195719"
FT   DOMAIN          55..148
FT                   /note="BLUF 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00030"
FT   DOMAIN          204..332
FT                   /note="Guanylate cyclase 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00099"
FT   DOMAIN          467..559
FT                   /note="BLUF 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00030"
FT   DOMAIN          615..744
FT                   /note="Guanylate cyclase 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00099"
FT   REGION          825..863
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        845..859
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1019 AA;  111638 MW;  1C83E3F74224E879 CRC64;
     MYILVWKEGQ QIRTFQDLEE SVQFRTASNI TDGQIFSINV TPTMGKGGET GETQLRRLMY
     LSASTEPENC NADYVGDMAH FATLRNKSIG VSGFYMYSAP FLFQVIQGTD EDLDFLCANG
     SADPRHQRCI ELADGPTTGR MYGWWHLKDS HIDNITKDPA IKTILIEIAR SFSSMWSYLP
     RNAANMVLLG KNPNKQAPEP MSVVVTVIYL VEFSVIMAHP GLSEQCPDIL PAFVDACVPH
     VDGTGGEVAK FIIAICMAYW PINSDEHALV GLQQLSDDLA ELRSLQEDGS ARSLIYSRCG
     LHFGRAMLCN AGFRKADFTL LGDWINSASR ITSLSVNSKV PLLLSFLVRC LLGDEMRVEL
     ERSGLHKVGG RVKPVQVYQF NAPELDTATV RGEIKQFNPG RERALCPVKP YESLHPAHPD
     PIFDDTPREN QPELSQVQRR DSLVDRLSQI AKLAFPSSMM AGGEGQLITL TYISQAAHPV
     SRLDLASIQR IAFARNESSN ITKSLLYVSG LFVQTLEGTK GAVVSLYLKN RQDKRHKDVV
     AAFMAPIDEG VYGSPLDMTS ATEEMLATFT PLHDVLSQLA KSFISLETYV PSTVVRHLAA
     GNNPRNLQPV SVEVVMLATA ISSFTPLSEK CSLTEVWTIC NTFIVACTSA ICNEGGEVIK
     LIGDCVPAYF PPTNADNAVH ACQEIVSFCA QLRAAFHDVL DCRSVVACGV GLDYGQVIMV
     QCGSLGMTEF VVAGEVRARV LEVEALTREA GRAIVITEPV PERQSPKLRD TGIVPCQEGV
     DGARCYGILG PEWELDVATI KKNINGFLKD ARALAANKKV DDGTNISCRG GNPPAGGIPT
     SPKVRPPGRT NSVSSYTPDP KQALDPRMAE SVFLDMCHQG DTVNNSIAVK LGLAANEDGF
     DGGWILTWYV ELMPVKQAIK VLTQLRIGNM SDNFVDDNNV GELLEKCIPT RSLQVLDLSN
     NPGLTKVIAL KPKHNTQVRE ILLNGTRIAP TEQRKQQTSK NVNRLCASTD LKGSHKYEH