PAL2_ORYSI
ID PAL2_ORYSI Reviewed; 713 AA.
AC A2X7F7; P53443; Q6K6P7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Phenylalanine ammonia-lyase;
DE EC=4.3.1.24 {ECO:0000250|UniProtKB:P24481};
GN Name=ZB8; ORFNames=OsI_008000;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. IR36;
RX PubMed=8534851; DOI=10.1007/bf00020983;
RA Zhu Q., Dabi T., Beeche A., Yamamoto R., Lawton M.A., Lamb C.;
RT "Cloning and properties of a rice gene encoding phenylalanine ammonia-
RT lyase.";
RL Plant Mol. Biol. 29:535-550(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: This is a key enzyme of plant metabolism catalyzing the first
CC reaction in the biosynthesis from L-phenylalanine of a wide variety of
CC natural products based on the phenylpropane skeleton.
CC {ECO:0000250|UniProtKB:P24481}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-phenylalanine = (E)-cinnamate + NH4(+);
CC Xref=Rhea:RHEA:21384, ChEBI:CHEBI:15669, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:58095; EC=4.3.1.24;
CC Evidence={ECO:0000250|UniProtKB:P24481};
CC -!- PATHWAY: Phenylpropanoid metabolism; trans-cinnamate biosynthesis;
CC trans-cinnamate from L-phenylalanine: step 1/1. {ECO:0000305}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:P24481}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Contains an active site 4-methylidene-imidazol-5-one (MIO), which
CC is formed autocatalytically by cyclization and dehydration of residues
CC Ala-Ser-Gly. {ECO:0000250|UniProtKB:Q68G84}.
CC -!- SIMILARITY: Belongs to the PAL/histidase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA61198.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA61198.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; X87946; CAA61198.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CM000127; EAY86767.1; -; Genomic_DNA.
DR PIR; S66313; S66313.
DR AlphaFoldDB; A2X7F7; -.
DR SMR; A2X7F7; -.
DR STRING; 39946.A2X7F7; -.
DR PRIDE; A2X7F7; -.
DR EnsemblPlants; BGIOSGA005998-TA; BGIOSGA005998-PA; BGIOSGA005998.
DR Gramene; BGIOSGA005998-TA; BGIOSGA005998-PA; BGIOSGA005998.
DR HOGENOM; CLU_014801_3_0_1; -.
DR OMA; CNEMIDP; -.
DR UniPathway; UPA00713; UER00725.
DR Proteomes; UP000007015; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0045548; F:phenylalanine ammonia-lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0009800; P:cinnamic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006559; P:L-phenylalanine catabolic process; IEA:UniProtKB-KW.
DR CDD; cd00332; PAL-HAL; 1.
DR Gene3D; 1.10.274.20; -; 1.
DR Gene3D; 1.10.275.10; -; 1.
DR InterPro; IPR001106; Aromatic_Lyase.
DR InterPro; IPR024083; Fumarase/histidase_N.
DR InterPro; IPR008948; L-Aspartase-like.
DR InterPro; IPR022313; Phe/His_NH3-lyase_AS.
DR InterPro; IPR005922; Phe_NH3-lyase.
DR InterPro; IPR023144; Phe_NH3-lyase_shielding_dom_sf.
DR PANTHER; PTHR10362; PTHR10362; 1.
DR Pfam; PF00221; Lyase_aromatic; 1.
DR SUPFAM; SSF48557; SSF48557; 1.
DR TIGRFAMs; TIGR01226; phe_am_lyase; 1.
DR PROSITE; PS00488; PAL_HISTIDASE; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Lyase; Phenylalanine catabolism; Phenylpropanoid metabolism;
KW Reference proteome.
FT CHAIN 1..713
FT /note="Phenylalanine ammonia-lyase"
FT /id="PRO_0000300256"
FT ACT_SITE 106
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q68G84"
FT BINDING 257
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q68G84"
FT BINDING 345
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q68G84"
FT BINDING 351
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q68G84"
FT BINDING 381
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q68G84"
FT BINDING 484
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q68G84"
FT MOD_RES 200
FT /note="2,3-didehydroalanine (Ser)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10122"
FT CROSSLNK 199..201
FT /note="5-imidazolinone (Ala-Gly)"
FT /evidence="ECO:0000250|UniProtKB:Q68G84"
SQ SEQUENCE 713 AA; 76937 MW; 00394D799E57673F CRC64;
MECENGRVSA NGMSGLCVAA PRADPLNWGK ATEEMTGSHL DEVKRMVAEY RQPLVKIEGA
SLRIAQVAAV AAAGEARVEL DESARERVKA SSDWVMNSMM NGTDSYGVTT GFGATSHRRT
KEGGALQREL IRFLNAGAFG TGTDGHVLPA EATRAAMLVR INTLLQGYSG IRFEILEAIA
KLLNANVTPC LPLRGTITAS GDLVPLSYIA GLVTGRENAV AVAPDGSKVN AAEAFKIAGI
QGGFFELQPK EGLAMVNGTA VGSGLASTVL FEANILAILA EVLSAVFCEV MNGKPEYTDH
LTHKLKHHPG QIEAAAIMEH ILEGSSYMKH AKKLGELDPL MKPKQDRYAL RTSPQWLGPQ
IEVIRAATKS IEREINSVND NPLIDVSRGK ALHGGNFQGT PIGVSMDNTR LAIAAIGKLM
FAQFSELVND FYNNGLPSNL SGGRNPSLDY GFKGAEIAMA SYCSELQFLG NPVTNHVQSA
EQHNQDVNSL GLISSRKTAE AIDILKLMSS TFLIALCQAV DLRHIEENVK SAVKSCVMTV
AKKTLSTNST GDLHVARFCE KDLLKEIDRE AVFAYADDPC SHNYPLMKKL RNVLVERALA
NGAAEFNADT SVFAKVAQFE EELRATLPGA IEAARAAVEN GTAAIPSRIT ECRSYPLYRF
VREELGTKYL TGEKTRSPGE ELNKVLVAIN EGKHIDPLLE CLKEWNGEPL PIC
