ID   PALA_ASPOR              Reviewed;         828 AA.
AC   Q5KU05; Q2UG75;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 2.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=pH-response regulator protein palA/RIM20;
GN   Name=palA; ORFNames=AO090023000950;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Sano M., Takase K., Yamamoto M., Machida M.;
RT   "Aspergillus oryzae palA.";
RL   Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA   Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA   Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA   Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA   Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA   Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA   Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA   Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA   Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA   Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA   Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA   Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
CC   -!- FUNCTION: Required for the proteolytic cleavage of the transcription
CC       factor pacC in response to alkaline ambient pH. May act as a scaffold
CC       protein that recruits the calpain-like protease palB via vps32 to its
CC       substrate pacC (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with pacC by binding to its two YPX[LI] motifs.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the palA/RIM20 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAE59440.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB062767; BAD83606.1; -; Genomic_DNA.
DR   EMBL; AP007157; BAE59440.1; ALT_SEQ; Genomic_DNA.
DR   AlphaFoldDB; Q5KU05; -.
DR   SMR; Q5KU05; -.
DR   STRING; 510516.Q5KU05; -.
DR   EnsemblFungi; BAE59440; BAE59440; AO090023000950.
DR   Proteomes; UP000006564; Chromosome 3.
DR   GO; GO:0071985; P:multivesicular body sorting pathway; IEA:InterPro.
DR   Gene3D; 1.25.40.280; -; 1.
DR   InterPro; IPR025304; ALIX_V_dom.
DR   InterPro; IPR045251; BRO1-like.
DR   InterPro; IPR004328; BRO1_dom.
DR   InterPro; IPR038499; BRO1_sf.
DR   PANTHER; PTHR23030; PTHR23030; 1.
DR   Pfam; PF13949; ALIX_LYPXL_bnd; 1.
DR   Pfam; PF03097; BRO1; 1.
DR   SMART; SM01041; BRO1; 1.
DR   PROSITE; PS51180; BRO1; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Reference proteome.
FT   CHAIN           1..828
FT                   /note="pH-response regulator protein palA/RIM20"
FT                   /id="PRO_0000218874"
FT   DOMAIN          4..397
FT                   /note="BRO1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00526"
FT   REGION          743..828
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        755..787
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        593..596
FT                   /note="LQGW -> ALLK (in Ref. 1; BAD83606)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   828 AA;  93333 MW;  CBA12E29A71D7E24 CRC64;
     MTSNILQIPF RRSHTVSLSD AITQYISTKY DQRPDMFADD LLIIDRLRNE AIHVQEPHVS
     GISRLVTYAA QLKWLGGKFP VDIGVEFPWY PAFGFNTSRP ISQNNIRFEL ANILFNLVAL
     YSQLAFSVNR TTPDGLKQAC NYLCQAAGVL AHLRADILPD LRASPPEDMD DMTLQSLEQL
     LLAQGQECFW QKAVKDGLKD ASIARLAAKV SDFYAEGGDY AVQSNAISPE WIHHMTAKHH
     HFAAAAQYRQ SLDCLEKRKY GEEVARLRDS EVCVNEALKE SRWINRTVLG DLQGLKNRVT
     EDLKRAEKDN DVIYLNPVPP KSELKIIDRA CMVAAKAPSQ VTDAISMLGD NGPLGQPLFS
     KLVPYAVHIA ASIYSDRRDR LVNETIIGEL ETMTDKLRDL LSSLNLPGSL QALEKPLGLP
     PTLVSHAEEM RQQDGLNRLR RSLEDTARVK ANDKAAYNEG VELLAAEKAE DDSSRRKYGT
     DRWAREPSEA AASKLYTTAR EIDGYFSSAQ SSDNLVEQKL RDSEAVFRVL TGTNRDLEMY
     VPSSRRAAIP PEVERESIRL RGCLSEVSRL ENRRKRRAQA LKDKARTDDI SKLQGWEAAR
     LEREFPMQAI QASQFEDLFE EQLHLYDTDL EMVTQEQHEQ DQISAQVREA NRAFTRAHTG
     DASTKEREKA LQELENGYLK YKEIISNIEV GRKFYNDLAK IVGRFRDDCK AFVHQRRMEA
     SQIEGDITSV AAMASLNLSQ PHLRQYSQQP TQPAQPVQPA QPVQPPNQLP PQPQPVQHQP
     PRDEPLTAPQ PTRANTRPSM APGVWSPEMG IRFGAQGTWD PSKGVKFS