ID   PALA_CRYNJ              Reviewed;         902 AA.
AC   P0CM46; Q55NW1; Q5KEK0;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=pH-response regulator protein palA/RIM20;
GN   Name=RIM20; OrderedLocusNames=CNG00250;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Required for the proteolytic cleavage of the transcription
CC       factor RIM101 in response to alkaline ambient pH. May act as a scaffold
CC       protein that recruits the calpain-like protease RIM13 via VPS32 to its
CC       substrate RIM101 (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with RIM101 by binding to its YPX[LI] motif.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the palA/RIM20 family. {ECO:0000305}.
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DR   EMBL; AE017347; AAW44451.1; -; Genomic_DNA.
DR   RefSeq; XP_571758.1; XM_571758.1.
DR   AlphaFoldDB; P0CM46; -.
DR   SMR; P0CM46; -.
DR   STRING; 5207.AAW44451; -.
DR   PaxDb; P0CM46; -.
DR   EnsemblFungi; AAW44451; AAW44451; CNG00250.
DR   GeneID; 3258948; -.
DR   KEGG; cne:CNG00250; -.
DR   VEuPathDB; FungiDB:CNG00250; -.
DR   eggNOG; KOG2220; Eukaryota.
DR   HOGENOM; CLU_007181_2_1_1; -.
DR   InParanoid; P0CM46; -.
DR   OMA; SEWIHHM; -.
DR   OrthoDB; 550620at2759; -.
DR   Proteomes; UP000002149; Chromosome 7.
DR   GO; GO:0005768; C:endosome; IBA:GO_Central.
DR   GO; GO:0071985; P:multivesicular body sorting pathway; IEA:InterPro.
DR   GO; GO:0042306; P:regulation of protein import into nucleus; IMP:CACAO.
DR   Gene3D; 1.25.40.280; -; 1.
DR   InterPro; IPR025304; ALIX_V_dom.
DR   InterPro; IPR045251; BRO1-like.
DR   InterPro; IPR004328; BRO1_dom.
DR   InterPro; IPR038499; BRO1_sf.
DR   PANTHER; PTHR23030; PTHR23030; 1.
DR   Pfam; PF13949; ALIX_LYPXL_bnd; 1.
DR   Pfam; PF03097; BRO1; 1.
DR   SMART; SM01041; BRO1; 1.
DR   PROSITE; PS51180; BRO1; 1.
PE   3: Inferred from homology;
KW   Reference proteome.
FT   CHAIN           1..902
FT                   /note="pH-response regulator protein palA/RIM20"
FT                   /id="PRO_0000218877"
FT   DOMAIN          3..415
FT                   /note="BRO1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00526"
FT   REGION          744..902
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        744..794
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        825..839
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        846..869
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        874..892
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   902 AA;  99638 MW;  7AEDEF93BA1D8BC4 CRC64;
     MSNFLPIPTK AATPLPSFAK HLLDYISAHF RDTHSEAFRK DVDVLVGMRK DWVEAKLEAH
     PEIIRAFMRY HAQLAFLSTK FPSDINLPFA YYLPFPATFS LSPDAPISLS SLTFERACVL
     FNMTALYASM AAAERRAEAE GIKRALGYLT AAAGVLEYLI TSVLPTLRSE LSSPQAAGYD
     MTESFLGTLK EFVLAEAQEC YWQQAVLQGT YKNGLIGKLS MKVSEYYKAA LASMNGTDYP
     SSSYFPLNWT AHMNVKQMHF EAAAQFRLSQ EDLEKSRYGE EIGRLKVAES LAKKGLDAAR
     KGVADSVVSD LKQLHAAIKS SLERAVRDND LVYVQPIPPA NQLAPIVGVG MVKVNVPAEV
     AEPVAWLMGG KAGMEPLFSG LVPYGVHLAL SIYDDRKDTL VRDLDGKREE LDGLAASTLQ
     SLNLPGSIQA LDRPVGLPPS LLKKSEEVAS SGGIERIRSL LDEVNRLAHA NVQSLNEAMD
     ILDQEATENE SLIARQPELQ QTRQPSHVAN QPLIQMAEQY EATIKQAGGS DATVRAKWEE
     WARLVGILAA GEMEMEDYVP GTTSPSGSLP PSVRPLRASL EELDDRIAHR ARLVREARQI
     SAADDIRPEV LKEAAKLAHG GSGDVKTEWF EDLFEKGLEK YMGVKREMDE EVAKHDELLE
     QIRTQNESFL SERKDDPIIK ERERRLQDMD LAYWKWREIV DNAEEGIKFY NSFAEMLHGF
     KAACGQFLNT RRIDVGQMTT QFQQQMNVSE PQQQPEPRYQ SPSPQSFQPS FSPSPSHFQP
     SAPSSFSSSP VRSPAPSAPA RRESPPKTLS FLPHPSSSAW QPASVDFLPP PPPPPILRSG
     GIQSQPKVAP PVTPSITAIR DQTQGTPRRM TRAAAAAAER DEATERNKYS SGGPRRKGGG
     VV