ID   PALA_YARLI              Reviewed;         773 AA.
AC   Q8WZL4; Q6C8F3;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 2.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=pH-response regulator protein RIM20;
GN   Name=RIM20; OrderedLocusNames=YALI0D20130g;
OS   Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Dipodascaceae; Yarrowia.
OX   NCBI_TaxID=284591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=SY12;
RX   PubMed=11861549; DOI=10.1093/genetics/160.2.417;
RA   Gonzalez-Lopez C.I., Szabo R., Blanchin-Roland S., Gaillardin C.;
RT   "Genetic control of extracellular protease synthesis in the yeast Yarrowia
RT   lipolytica.";
RL   Genetics 160:417-427(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIB 122 / E 150;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Required for the proteolytic cleavage of the transcription
CC       factor RIM101 in response to alkaline ambient pH. May act as a scaffold
CC       protein that recruits the calpain-like protease RIM13 via VPS32 to its
CC       substrate RIM101 (By similarity). {ECO:0000250,
CC       ECO:0000269|PubMed:11861549}.
CC   -!- SUBUNIT: Interacts with RIM101 by binding to its two YPX[LI] motifs.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the palA/RIM20 family. {ECO:0000305}.
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DR   EMBL; AJ319901; CAC86009.2; -; Genomic_DNA.
DR   EMBL; CR382130; CAG81251.1; -; Genomic_DNA.
DR   RefSeq; XP_503059.1; XM_503059.1.
DR   AlphaFoldDB; Q8WZL4; -.
DR   SMR; Q8WZL4; -.
DR   STRING; 4952.CAG81251; -.
DR   PRIDE; Q8WZL4; -.
DR   EnsemblFungi; CAG81251; CAG81251; YALI0_D20130g.
DR   GeneID; 2910311; -.
DR   KEGG; yli:YALI0D20130g; -.
DR   VEuPathDB; FungiDB:YALI0_D20130g; -.
DR   HOGENOM; CLU_007181_2_1_1; -.
DR   InParanoid; Q8WZL4; -.
DR   OMA; SEWIHHM; -.
DR   Proteomes; UP000001300; Chromosome D.
DR   GO; GO:0005768; C:endosome; IBA:GO_Central.
DR   GO; GO:0071985; P:multivesicular body sorting pathway; IEA:InterPro.
DR   Gene3D; 1.25.40.280; -; 1.
DR   InterPro; IPR025304; ALIX_V_dom.
DR   InterPro; IPR045251; BRO1-like.
DR   InterPro; IPR004328; BRO1_dom.
DR   InterPro; IPR038499; BRO1_sf.
DR   PANTHER; PTHR23030; PTHR23030; 1.
DR   Pfam; PF13949; ALIX_LYPXL_bnd; 1.
DR   Pfam; PF03097; BRO1; 1.
DR   SMART; SM01041; BRO1; 1.
DR   PROSITE; PS51180; BRO1; 1.
PE   3: Inferred from homology;
KW   Reference proteome.
FT   CHAIN           1..773
FT                   /note="pH-response regulator protein RIM20"
FT                   /id="PRO_0000218884"
FT   DOMAIN          3..398
FT                   /note="BRO1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00526"
FT   REGION          726..758
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        726..742
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        743..758
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   773 AA;  87470 MW;  ADDBCCA925F0A903 CRC64;
     MPNIIWIPFR ETQAVDLITG LGDTIEKQLN QPRDKFTADL KTANDLRNNI LNPQPNASYL
     DHLTKYYAQL TYWTTKFPAG CDSLEFMWYG TLAYTANAAP VISQSLHFER CNLLYNLGSL
     YSQMGVNEGR QDADGLKMSF NYFQMAAGCF QILIENGLPD LESLNMRGLE YDTICCVRDL
     MLAQAQECFW QRSLLSGSRN SLVAKLAQQV SLLYDSALAW AQKSPQVRSE WVHHMTCKRQ
     HFLAVAHYRM ARDALDGCRY GEEIARLKAA LNAIKEGTGK GMLRRYLVES VADDLNGIID
     VVTEDLKRAE KDNNLIYLQS VPQTTALAPI TPALMVKCTP PVELAKPIEF LTKDKLGLPL
     FVKLTPFAFH QALVLYSQEC DKIVSSIESQ LKNCTNSKNQ ALVEMALPGS LDSVEKPKGI
     PADIIAHSDQ IKSERGLETT REAVKDVQRL AATARQELEA AKAVLDDERS EDEMLRRKNN
     SGWTRFDSKE AGAHLYTRLA ELEQYLADAG ASDKLVRGKL DEIDYLLSIL NSGRSALENY
     LPSSVSGVRS PEVKRQVPHL RDLLNNLESL EIKRAQYMDR LSLKQQNDDI EPVLEYHMNK
     LIREKHANLR LTLADFEPIT SKELEKYTVD QDWASEQKEL QEEVLEKLDV ANKDFRSSLD
     MNDAAIHREN AIQSLEVAYF SYQEQKDHLD AGRNFYNQLL GEISIFHKNC KQFVYERKME
     GASLEHELSS MHLSEHEHSG GHSDTSSTPL AAPRAQRASN LSMWSPEDGI RFG