ID   PALB_ASHGO              Reviewed;         694 AA.
AC   Q759K3;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Calpain-like protease palB/RIM13;
DE            EC=3.4.22.-;
DE   AltName: Full=Cysteine protease RIM13;
GN   Name=RIM13; OrderedLocusNames=ADR274C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 235-236; 238; 269 AND 273.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Required for the proteolytic cleavage of the transcription
CC       factor RIM101 in response to alkaline ambient pH. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase C2 family. PalB/RIM13 subfamily.
CC       {ECO:0000305}.
CC   -!- CAUTION: Lacks the conserved Asn residue of the catalytic triad in
CC       position 292, which is replaced by an Asp residue. {ECO:0000305}.
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DR   EMBL; AE016817; AAS52194.2; -; Genomic_DNA.
DR   RefSeq; NP_984370.2; NM_209723.2.
DR   AlphaFoldDB; Q759K3; -.
DR   SMR; Q759K3; -.
DR   STRING; 33169.AAS52194; -.
DR   EnsemblFungi; AAS52194; AAS52194; AGOS_ADR274C.
DR   GeneID; 4620532; -.
DR   KEGG; ago:AGOS_ADR274C; -.
DR   eggNOG; KOG0045; Eukaryota.
DR   HOGENOM; CLU_395483_0_0_1; -.
DR   InParanoid; Q759K3; -.
DR   Proteomes; UP000000591; Chromosome IV.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   SUPFAM; SSF54001; SSF54001; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Reference proteome; Thiol protease.
FT   CHAIN           1..694
FT                   /note="Calpain-like protease palB/RIM13"
FT                   /id="PRO_0000207736"
FT   DOMAIN          80..320
FT                   /note="Calpain catalytic"
FT   ACT_SITE        135
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        276
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   694 AA;  78836 MW;  1B5D62C479951178 CRC64;
     MEALLAVEET GNDLWKELKE LYWAQYVQNS VDKVQFERYV RKVKSADDYW LVRALLAFSD
     SRKACTFNSR FEWLTMQAHG RQYPPLASKD AGKLIWNARR EFRYAEPQQA AYKQLARPRG
     DAVADIQQHP DLGNCSMVCA LINVKRTCGT ADMVADCGDV AQVNLFFNGA RRLVTLHRAE
     VRQNMRREQL AVLSPDMHDK LIEQAYFEVA HGMNYESMGS NTAMDTYMLC GWFPLVVETS
     EVDFPFVQRY LARGAMLALG THATRTIDSF CLRKNHDYAV LDVCGTVFRI RDPLVAGNVI
     ELAWEDVRTA FHLLYINWDA HTQCQLYEKF HFRYNSAVHN RFMSWLNKPT YRVVNDTESE
     LPVYALFERH LSNRKYDNSI VGVISGENLV SDTEEGNNIG MQWVELTVPP HSSRILFYHS
     NTNTNCTLHL FSNSPRLKVA KWSSAESSRL AVLDGCWQPI SKNLTFTHWP AFKFEIKHPE
     TDGVHVSLQF CWANEHSAAL FIYHASDYRY EKPLLHRPGP LPFPQAFFEL ALDTNTPYVV
     LCQSMDVGAD AAFQLAIQTE KQGSVISTVP TSRAYGGLRF HTSKLVSTAA KRIELPFKVS
     RSTDLHITLY SKSSPLVLKC AVFSDGSDEA ITATPGFVPI NDYPYVLPIT FAKERSLVLL
     IELQDTVPDS TVTIEIGSNC AFNFCGHDVD LARV