ID   PALB_CRYNJ              Reviewed;         814 AA.
AC   P0CQ06; Q55IT8; Q5KCW3;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=Calpain-like protease palB/RIM13;
DE            EC=3.4.22.-;
DE   AltName: Full=Cysteine protease RIM13;
GN   Name=RIM13; OrderedLocusNames=CNH02440;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Required for the proteolytic cleavage of the transcription
CC       factor RIM101 in response to alkaline ambient pH. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase C2 family. PalB/RIM13 subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAW45112.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AE017348; AAW45112.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_572419.1; XM_572419.1.
DR   AlphaFoldDB; P0CQ06; -.
DR   SMR; P0CQ06; -.
DR   STRING; 5207.AAW45112; -.
DR   PaxDb; P0CQ06; -.
DR   EnsemblFungi; AAW45112; AAW45112; CNH02440.
DR   VEuPathDB; FungiDB:CNH02440; -.
DR   eggNOG; KOG0045; Eukaryota.
DR   InParanoid; P0CQ06; -.
DR   OrthoDB; 343870at2759; -.
DR   Proteomes; UP000002149; Chromosome 8.
DR   GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR   InterPro; IPR022683; Calpain_III.
DR   InterPro; IPR036213; Calpain_III_sf.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR001300; Peptidase_C2_calpain_cat.
DR   Pfam; PF00648; Peptidase_C2; 1.
DR   SMART; SM00720; calpain_III; 1.
DR   SMART; SM00230; CysPc; 1.
DR   SUPFAM; SSF49758; SSF49758; 3.
DR   SUPFAM; SSF54001; SSF54001; 1.
DR   PROSITE; PS50203; CALPAIN_CAT; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Reference proteome; Thiol protease.
FT   CHAIN           1..814
FT                   /note="Calpain-like protease palB/RIM13"
FT                   /id="PRO_0000207740"
FT   DOMAIN          118..400
FT                   /note="Calpain catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00239"
FT   ACT_SITE        185
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00239"
FT   ACT_SITE        354
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00239"
SQ   SEQUENCE   814 AA;  89673 MW;  3ADFDCBC54948BE1 CRC64;
     MPFCSYQEGL KVASDLGNKA IQTEASLSSL SPVASPIPTL QKAFPIYISA AEAYGHLLSS
     KLVPQSDIET VKRKWRLVLE RAEKVKSRIE QLGGHVAKAE IGDRGEEIAV LRRASLINGV
     AVELWRPPGD TFDAGEPYRE AVQPELAAAQ LDMDPEWRDI QADCWLHQAP NEGDWVMRQG
     PVSDCSVVAA MGVGIKHAGQ FGTAFGWENL YPQDAHGRPR RSKNGKHILK LLLNGAWRSV
     VLDSLLPFSK RDKTPLFTTC HPAPHILPTS VGSPWAPLAL KGYFKVHGGY SLRGSNPSSD
     IYEFMGWIPE RIGLKEGFQR EKEWKRMKEA WHKGNVMVSL GTGSKVSEGL VKLHAYGVIR
     LREEGHERIL DIFDPGATSF TMSWDQVCAE FEALHLNWKP SVMPNTATRH WSWPKPPDLP
     SDADPGMMNT RYRLHVNASS PSSSLSEVWI LLSQHITSRD RPLDDIALHV FEGLGPNNSR
     NLGAIYSEGL ERTNPYTNSN HLLVRYQLRR PTSDLTLIPS RDRGIDQTGF TLNAFAPASI
     SLSLERISRT LPFTQRISGN LTDRSAGGHP GWPTHMTNPQ YKVVVRPGEG KSEISGRIIL
     HGEKDVPWNV KLIWGRGQLV YELSEDLIVA DTGSYSYGIA YCDIPELQPD TYTLIVSAFE
     PGQTGLFSLS LEATAPVSII PIAAEGAGMY NRVVNGFWTE RTAGGRPSGG TYESNPRVEM
     VLSKPATIQS RLYLPTRSPV PINLTIFKRA QGGALGEQLV TTGPYSDSIC GVSTGKMKLD
     SGVYLLVPSS YEKSKGGWVL KIWSDVALSA EIVG