ID   PALB_KLULA              Reviewed;         685 AA.
AC   Q6CKY3;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Calpain-like protease palB/RIM13;
DE            EC=3.4.22.-;
DE   AltName: Full=Cysteine protease RIM13;
GN   Name=RIM13; OrderedLocusNames=KLLA0F07183g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Required for the proteolytic cleavage of the transcription
CC       factor RIM101 in response to alkaline ambient pH. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase C2 family. PalB/RIM13 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CR382126; CAG98114.1; -; Genomic_DNA.
DR   RefSeq; XP_455406.1; XM_455406.1.
DR   AlphaFoldDB; Q6CKY3; -.
DR   SMR; Q6CKY3; -.
DR   STRING; 28985.XP_455406.1; -.
DR   EnsemblFungi; CAG98114; CAG98114; KLLA0_F07183g.
DR   GeneID; 2895973; -.
DR   KEGG; kla:KLLA0_F07183g; -.
DR   eggNOG; KOG0045; Eukaryota.
DR   HOGENOM; CLU_023416_0_0_1; -.
DR   InParanoid; Q6CKY3; -.
DR   OMA; NISIDTY; -.
DR   Proteomes; UP000000598; Chromosome F.
DR   GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   SUPFAM; SSF54001; SSF54001; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Reference proteome; Thiol protease.
FT   CHAIN           1..685
FT                   /note="Calpain-like protease palB/RIM13"
FT                   /id="PRO_0000207743"
FT   DOMAIN          82..323
FT                   /note="Calpain catalytic"
FT   ACT_SITE        139
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        277
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        295
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   685 AA;  79686 MW;  5F81BCC6A6648724 CRC64;
     MPRGAQAMLE KQADSVWNQL YEICWNFYVK NVWDINTFKA IVDKIEKSDD KQLIRFLIWF
     NDARKRSNFN DKFQWMTSFL YGRRYPPLGD DLVVKEPWND LQQLDSIIKY GNEPIDLVKM
     QPDFTTEERI VQNRRVNNCS LICSLTNARE ENQIKSYSVH NVFNVILHFN GSNRLVSVLR
     DSNFPKCSKD GAALTLFSHN IEDMLIELAY FEIKNNRNYA YGGSNTATDT YLLTGWIPEI
     LNLGDVSFSE IRKCYQFNAM LALGTDSVKG KEVTENHDFV VTSVNWKKEQ FTILNPHGTE
     NPTVYTWDDL IKEFKWLYVN WDPYSKYTQR VKLHDRYPDK FNAYPTWTQK PLMCIENKSN
     SIQNCHLYLE RHLLDDSILE SNLTIGEIPR SGFTAVSAEG NNSGFCNMQL RLNPHETRLI
     FYHSDKKCNL TFHLLSNSGL VSMSKCKSDI LTAESSWNIT NNQWTIGTSE YFKSPVFKLK
     SDGFDDTYLD FELVSQLPAK VNFQIFESDD YALQRPIFKN DTYSPQIFSK HGVRLLKGRE
     YFIVCSIAHH ITSPFIFTLR NPETKQSKFE LSPYSLGFGG LRYNEVLRCK TRITISVSNF
     TRLFVLLFTS ASKTNGRLTL RLYEKETEEI LFEQIETRYD LSVKPFPIPN IELKPASYVL
     EIFYNADVVI CNLGTSHRAI FESDL