ID   PALFB_EUGGR             Reviewed;        1019 AA.
AC   P84737; Q2P9N0;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Photoactivated adenylate cyclase subunit alpha-like protein FB;
GN   Name=pacA {ECO:0000312|EMBL:CAJ57394.1};
OS   Euglena gracilis.
OC   Eukaryota; Discoba; Euglenozoa; Euglenida; Spirocuta; Euglenophyceae;
OC   Euglenales; Euglenaceae; Euglena.
OX   NCBI_TaxID=3039;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:CAJ57394.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RC   STRAIN=FB {ECO:0000269|PubMed:16121285};
RX   PubMed=16121285; DOI=10.1039/b502002f;
RA   Ntefidou M., Haeder D.-P.;
RT   "Photoactivated adenylyl cyclase (PAC) genes in the flagellate Euglena
RT   gracilis mutant strains.";
RL   Photochem. Photobiol. Sci. 4:732-739(2005).
CC   -!- SUBUNIT: Heterotetramer of two alpha and two beta subunits.
CC       {ECO:0000250|UniProtKB:Q8S9F2}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC       {ECO:0000269|PubMed:16121285}. Note=Paraxonemal body. And paraxonemal
CC       bodies (PABs).
CC   -!- MISCELLANEOUS: The FB strain is deficient in phototaxis. It is not
CC       known if this is due to defective adenylate cyclase activity or
CC       defective BLUF domains in this protein. In wild-type E.gracilis,
CC       photoactivated adenylate cyclase is found in the paraxonemal bodies
CC       (PABs). PABs are not visible in all cells in this strain, and are
CC       smaller than in wild-type.
CC   -!- SIMILARITY: Belongs to the adenylyl cyclase class-4/guanylyl cyclase
CC       family. {ECO:0000255|PROSITE-ProRule:PRU00099}.
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DR   EMBL; AM181335; CAJ57394.1; -; mRNA.
DR   AlphaFoldDB; P84737; -.
DR   SMR; P84737; -.
DR   PRIDE; P84737; -.
DR   GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR   GO; GO:0009882; F:blue light photoreceptor activity; IEA:InterPro.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0009190; P:cyclic nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR   CDD; cd07302; CHD; 2.
DR   Gene3D; 3.30.70.1230; -; 2.
DR   Gene3D; 3.80.10.10; -; 1.
DR   InterPro; IPR001054; A/G_cyclase.
DR   InterPro; IPR036046; Acylphosphatase-like_dom_sf.
DR   InterPro; IPR007024; BLUF_domain.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   InterPro; IPR029787; Nucleotide_cyclase.
DR   Pfam; PF04940; BLUF; 2.
DR   Pfam; PF00211; Guanylate_cyc; 2.
DR   SMART; SM01034; BLUF; 2.
DR   SUPFAM; SSF54975; SSF54975; 2.
DR   SUPFAM; SSF55073; SSF55073; 2.
DR   PROSITE; PS50925; BLUF; 2.
DR   PROSITE; PS50125; GUANYLATE_CYCLASE_2; 2.
PE   2: Evidence at transcript level;
KW   Cell projection; Cilium; Flagellum; Repeat.
FT   CHAIN           1..1019
FT                   /note="Photoactivated adenylate cyclase subunit alpha-like
FT                   protein FB"
FT                   /id="PRO_0000195720"
FT   DOMAIN          55..148
FT                   /note="BLUF 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00030"
FT   DOMAIN          204..332
FT                   /note="Guanylate cyclase 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00099"
FT   DOMAIN          467..559
FT                   /note="BLUF 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00030"
FT   DOMAIN          615..744
FT                   /note="Guanylate cyclase 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00099"
FT   REGION          825..862
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        842..856
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1019 AA;  112093 MW;  EFC2B34B7744A492 CRC64;
     MYILVWKEGQ QIRTFQDLEE CGQFQTASNI TDGQIFSINV TPTMSKGGET GETQLRRLMY
     LSASTEPEKC YAEYLADMAH VATLRNKQIG VSGFLLYSSP FFFEVIEGTD EELDFLFAKI
     SADPRHVRCI VLANGPCTGR LYGEWHMKDS HIDNITKHPA IKTILFQIAR SFSCMWSYLP
     KNAANMLLLG KNPNKQAPEP MSVVVTFIYL VEFSSILAHP GQTEQCADIL AAFVDACVRN
     VEGTGAQVAK FITGICIAYW PINRAEDALI GLQQLSEDLA ELRSQQRPGS ALSLIYSRCG
     VHYGRALLCN AGFRKADFTL LGDCINTASR ITSLSVKLKV PLLLSFEVRC VLGDEMREVL
     ETSGLHKVKG RDQPVQVYQF NARELDSAMV RAKIEQLNPG SYRALCPVKP YESLLPAQRP
     PIFDDTPREN QPKLSQVQRR DSLVDRLSLI AKLAFPTSMM AGGEGQLITL TYISQAAHPM
     TRLDLASIQR IAFARNESSN ITGSLLHVSG LFVQTLEGPK GAVVSLYLKI RQDKRHKDVV
     ADFMAPSEGR EYGSPLDMTS ATEEMLTSFP PLQELLTHLA KPFISLETYV PTTVVRYLTA
     GNNPRNLQPV SVEVVLLATD ICSFTPLSEK CSLTEVWTIC KTIFVAFIRA IFNEGGEVIK
     LIGDCVTAYF PPTGADKAVH ACQEIVTFCA QLGDAFHDVL DCRSVVACGV GLDFGQVIMA
     QCGSLGMTEF VVAGEVSARV MEVVALTREA GRAIVITEPL PDRLAPKLRD TGIIPCQEGV
     DGVPCYGNLG PEWELDVATI KKNIYGFHDA RALAAMKKVD VGTNAPGRGA PAGGIPSSPK
     VRPPGRTNSV SSYTPDPNEA LDPPMAESVF LDMCHQRGDT ANNSIAVKLR QAANDDRLDL
     GRMLQGPHEL MPVMQAIKHL TNLRMLNMSD NFVVDNNVGE LVESCIPMRS LQVLDLSNNP
     GLTKVIALKR LIKHNTQVRE FLLNGTRIAP TEQRKLQSSM NVNRLCASTD LKGSHKYEH