PALS2_HUMAN
ID PALS2_HUMAN Reviewed; 540 AA.
AC Q9NZW5; A4D157; Q9H0E1;
DT 10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 16-FEB-2004, sequence version 2.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Protein PALS2 {ECO:0000305};
DE AltName: Full=MAGUK p55 subfamily member 6;
DE AltName: Full=Membrane protein, palmitoylated 6;
DE AltName: Full=Veli-associated MAGUK 1 {ECO:0000303|PubMed:11311936};
DE Short=VAM-1 {ECO:0000303|PubMed:11311936};
GN Name=PALS2 {ECO:0000312|HGNC:HGNC:18167}; Synonyms=MPP6, VAM1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11311936; DOI=10.1016/s0167-4781(01)00191-9;
RA Tseng T.-C., Marfatia S.M., Bryant P.J., Pack S., Zhuang Z., O'Brien J.E.,
RA Lin L., Hanada T., Chishti A.H.;
RT "VAM-1: a new member of the MAGUK family binds to human Veli-1 through a
RT conserved domain.";
RL Biochim. Biophys. Acta 1518:249-259(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-500, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=15592455; DOI=10.1038/nbt1046;
RA Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H.,
RA Zha X.-M., Polakiewicz R.D., Comb M.J.;
RT "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.";
RL Nat. Biotechnol. 23:94-101(2005).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- SUBUNIT: Interacts with CADM1 (By similarity). Interacts with the LIN7
CC proteins. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9NZW5; O14910: LIN7A; NbExp=4; IntAct=EBI-2683764, EBI-2513988;
CC Q9NZW5; Q9HAP6: LIN7B; NbExp=5; IntAct=EBI-2683764, EBI-821335;
CC Q9NZW5; Q9NUP9: LIN7C; NbExp=6; IntAct=EBI-2683764, EBI-1171517;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Peripheral membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Abundant in testis, brain, and kidney with lower
CC levels detectable in other tissues.
CC -!- SIMILARITY: Belongs to the MAGUK family. {ECO:0000305}.
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DR EMBL; AF162130; AAD45919.2; -; mRNA.
DR EMBL; AL136836; CAB66770.1; -; mRNA.
DR EMBL; AC005084; AAQ96847.1; -; Genomic_DNA.
DR EMBL; CH236948; EAL24247.1; -; Genomic_DNA.
DR EMBL; CH471073; EAW93810.1; -; Genomic_DNA.
DR EMBL; BC023638; AAH23638.1; -; mRNA.
DR CCDS; CCDS5388.1; -.
DR RefSeq; NP_001289966.1; NM_001303037.1.
DR RefSeq; NP_057531.2; NM_016447.3.
DR RefSeq; XP_006715801.1; XM_006715738.3.
DR RefSeq; XP_006715802.1; XM_006715739.3.
DR RefSeq; XP_006715803.1; XM_006715740.3.
DR RefSeq; XP_011513727.1; XM_011515425.2.
DR RefSeq; XP_016867804.1; XM_017012315.1.
DR RefSeq; XP_016867805.1; XM_017012316.1.
DR RefSeq; XP_016867806.1; XM_017012317.1.
DR AlphaFoldDB; Q9NZW5; -.
DR SMR; Q9NZW5; -.
DR BioGRID; 119675; 63.
DR CORUM; Q9NZW5; -.
DR IntAct; Q9NZW5; 20.
DR STRING; 9606.ENSP00000222644; -.
DR GlyGen; Q9NZW5; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9NZW5; -.
DR MetOSite; Q9NZW5; -.
DR PhosphoSitePlus; Q9NZW5; -.
DR SwissPalm; Q9NZW5; -.
DR BioMuta; MPP6; -.
DR DMDM; 42560556; -.
DR EPD; Q9NZW5; -.
DR jPOST; Q9NZW5; -.
DR MassIVE; Q9NZW5; -.
DR MaxQB; Q9NZW5; -.
DR PaxDb; Q9NZW5; -.
DR PeptideAtlas; Q9NZW5; -.
DR PRIDE; Q9NZW5; -.
DR ProteomicsDB; 83523; -.
DR Antibodypedia; 12204; 252 antibodies from 35 providers.
DR DNASU; 51678; -.
DR Ensembl; ENST00000222644.10; ENSP00000222644.4; ENSG00000105926.16.
DR Ensembl; ENST00000396475.6; ENSP00000379737.2; ENSG00000105926.16.
DR GeneID; 51678; -.
DR KEGG; hsa:51678; -.
DR MANE-Select; ENST00000222644.10; ENSP00000222644.4; NM_001303037.2; NP_001289966.1.
DR UCSC; uc003swx.4; human.
DR CTD; 51678; -.
DR DisGeNET; 51678; -.
DR GeneCards; PALS2; -.
DR HGNC; HGNC:18167; PALS2.
DR HPA; ENSG00000105926; Tissue enhanced (brain, testis).
DR MIM; 606959; gene.
DR neXtProt; NX_Q9NZW5; -.
DR OpenTargets; ENSG00000105926; -.
DR VEuPathDB; HostDB:ENSG00000105926; -.
DR eggNOG; KOG0609; Eukaryota.
DR GeneTree; ENSGT00940000158500; -.
DR InParanoid; Q9NZW5; -.
DR OMA; NPTTPHK; -.
DR OrthoDB; 847888at2759; -.
DR PhylomeDB; Q9NZW5; -.
DR TreeFam; TF314263; -.
DR PathwayCommons; Q9NZW5; -.
DR SignaLink; Q9NZW5; -.
DR BioGRID-ORCS; 51678; 33 hits in 1079 CRISPR screens.
DR ChiTaRS; MPP6; human.
DR GeneWiki; MPP6; -.
DR GenomeRNAi; 51678; -.
DR Pharos; Q9NZW5; Tbio.
DR PRO; PR:Q9NZW5; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q9NZW5; protein.
DR Bgee; ENSG00000105926; Expressed in calcaneal tendon and 116 other tissues.
DR ExpressionAtlas; Q9NZW5; baseline and differential.
DR Genevisible; Q9NZW5; HS.
DR GO; GO:0005911; C:cell-cell junction; IBA:GO_Central.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0016020; C:membrane; NAS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0065003; P:protein-containing complex assembly; NAS:UniProtKB.
DR CDD; cd12038; SH3_MPP6; 1.
DR Gene3D; 2.30.42.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR008145; GK/Ca_channel_bsu.
DR InterPro; IPR008144; Guanylate_kin-like_dom.
DR InterPro; IPR020590; Guanylate_kinase_CS.
DR InterPro; IPR014775; L27_C.
DR InterPro; IPR004172; L27_dom.
DR InterPro; IPR036892; L27_dom_sf.
DR InterPro; IPR035603; MPP6_SH3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR Pfam; PF00625; Guanylate_kin; 1.
DR Pfam; PF02828; L27; 2.
DR Pfam; PF00595; PDZ; 1.
DR Pfam; PF07653; SH3_2; 1.
DR SMART; SM00072; GuKc; 1.
DR SMART; SM00569; L27; 2.
DR SMART; SM00228; PDZ; 1.
DR SMART; SM00326; SH3; 1.
DR SUPFAM; SSF101288; SSF101288; 1.
DR SUPFAM; SSF50044; SSF50044; 1.
DR SUPFAM; SSF50156; SSF50156; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00856; GUANYLATE_KINASE_1; 1.
DR PROSITE; PS50052; GUANYLATE_KINASE_2; 1.
DR PROSITE; PS51022; L27; 2.
DR PROSITE; PS50106; PDZ; 1.
DR PROSITE; PS50002; SH3; 1.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Repeat; SH3 domain.
FT CHAIN 1..540
FT /note="Protein PALS2"
FT /id="PRO_0000094584"
FT DOMAIN 1..48
FT /note="L27 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00365"
FT DOMAIN 49..107
FT /note="L27 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00365"
FT DOMAIN 130..209
FT /note="PDZ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT DOMAIN 215..284
FT /note="SH3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT DOMAIN 338..525
FT /note="Guanylate kinase-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00100"
FT MOD_RES 500
FT /note="Phosphotyrosine"
FT /evidence="ECO:0007744|PubMed:15592455"
FT CONFLICT 41
FT /note="H -> R (in Ref. 1; AAD45919)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 540 AA; 61117 MW; 86310E4F6AF632BD CRC64;
MQQVLENLTE LPSSTGAEEI DLIFLKGIME NPIVKSLAKA HERLEDSKLE AVSDNNLELV
NEILEDITPL INVDENVAEL VGILKEPHFQ SLLEAHDIVA SKCYDSPPSS PEMNNSSINN
QLLPVDAIRI LGIHKRAGEP LGVTFRVENN DLVIARILHG GMIDRQGLLH VGDIIKEVNG
HEVGNNPKEL QELLKNISGS VTLKILPSYR DTITPQQVFV KCHFDYNPYN DNLIPCKEAG
LKFSKGEILQ IVNREDPNWW QASHVKEGGS AGLIPSQFLE EKRKAFVRRD WDNSGPFCGT
ISSKKKKKMM YLTTRNAEFD RHEIQIYEEV AKMPPFQRKT LVLIGAQGVG RRSLKNRFIV
LNPTRFGTTV PFTSRKPRED EKDGQAYKFV SRSEMEADIK AGKYLEHGEY EGNLYGTKID
SILEVVQTGR TCILDVNPQA LKVLRTSEFM PYVVFIAAPE LETLRAMHKA VVDAGITTKL
LTDSDLKKTV DESARIQRAY NHYFDLIIIN DNLDKAFEKL QTAIEKLRME PQWVPISWVY
