PAM17_YEAST
ID PAM17_YEAST Reviewed; 197 AA.
AC P36147; D6VXC6;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Presequence translocated-associated motor subunit PAM17, mitochondrial;
DE Flags: Precursor;
GN Name=PAM17; Synonyms=FMP18; OrderedLocusNames=YKR065C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE PAM COMPLEX,
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16107694; DOI=10.1128/mcb.25.17.7449-7458.2005;
RA van der Laan M., Chacinska A., Lind M., Perschil I., Sickmann A.,
RA Meyer H.E., Guiard B., Meisinger C., Pfanner N., Rehling P.;
RT "Pam17 is required for architecture and translocation activity of the
RT mitochondrial protein import motor.";
RL Mol. Cell. Biol. 25:7449-7458(2005).
CC -!- FUNCTION: Component of the PAM complex, a complex required for the
CC translocation of transit peptide-containing proteins from the inner
CC membrane into the mitochondrial matrix in an ATP-dependent manner. In
CC the complex, it is required to organize PAM16-PAM18 (TIM16-TIM14)
CC heterodimer. {ECO:0000269|PubMed:16107694}.
CC -!- SUBUNIT: Component of the PAM complex, at least composed of mtHsp70,
CC MGE1, TIM44, PAM16, PAM17 and PAM18/TIM14.
CC {ECO:0000269|PubMed:16107694}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 3000 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the PAM17 family. {ECO:0000305}.
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DR EMBL; Z28290; CAA82144.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09216.1; -; Genomic_DNA.
DR PIR; S38141; S38141.
DR RefSeq; NP_012991.3; NM_001179855.3.
DR AlphaFoldDB; P36147; -.
DR BioGRID; 34196; 267.
DR ComplexPortal; CPX-539; TIM23 mitochondrial inner membrane pre-sequence translocase complex, motor variant.
DR DIP; DIP-2128N; -.
DR IntAct; P36147; 5.
DR MINT; P36147; -.
DR STRING; 4932.YKR065C; -.
DR iPTMnet; P36147; -.
DR MaxQB; P36147; -.
DR PaxDb; P36147; -.
DR PRIDE; P36147; -.
DR EnsemblFungi; YKR065C_mRNA; YKR065C; YKR065C.
DR GeneID; 853939; -.
DR KEGG; sce:YKR065C; -.
DR SGD; S000001773; PAM17.
DR VEuPathDB; FungiDB:YKR065C; -.
DR eggNOG; ENOG502S1B1; Eukaryota.
DR HOGENOM; CLU_068297_2_0_1; -.
DR InParanoid; P36147; -.
DR OMA; YKPSVNT; -.
DR BioCyc; YEAST:G3O-32033-MON; -.
DR PRO; PR:P36147; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36147; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0001405; C:PAM complex, Tim23 associated import motor; IDA:SGD.
DR GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IC:ComplexPortal.
DR GO; GO:0006886; P:intracellular protein transport; IC:ComplexPortal.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IMP:SGD.
DR InterPro; IPR013875; Pam17.
DR PANTHER; PTHR28021; PTHR28021; 1.
DR Pfam; PF08566; Pam17; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW Reference proteome; Transit peptide; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT TRANSIT 1..14
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 15..197
FT /note="Presequence translocated-associated motor subunit
FT PAM17, mitochondrial"
FT /id="PRO_0000043179"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 197 AA; 21968 MW; 633C74D569E51603 CRC64;
MFTSAIRLSS QRLFASQPSV TAAALRSTAT TLPLRSYSQP ASLQDSSILT WSDFFKLRKQ
QRRINVGSSL FTALLGCNVS WAYLSTMEID PTQMLFGFDP LTVISAGIIA SGALGYLLGP
IVGSQVFKLS HNQQLAQFNN KNKEFLKHII NNRVDASSQS FSNPVPDYYG EKIGSLKEYK
QWLRDCHAYA KKAKEFL
