PAM71_ARATH
ID PAM71_ARATH Reviewed; 370 AA.
AC Q94AX5; Q9SH65;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Protein PAM71, chloroplastic {ECO:0000303|PubMed:27020959};
DE AltName: Full=CA(2+)/H(+) ANTIPORTER 1 {ECO:0000303|PubMed:27302341};
DE AltName: Full=GDT1-like protein 1 {ECO:0000305};
DE AltName: Full=PHOTOSYNTHESIS AFFECTED MUTANT71 {ECO:0000303|PubMed:27020959};
DE Flags: Precursor;
GN Name=PAM71 {ECO:0000303|PubMed:27020959};
GN Synonyms=CCHA1 {ECO:0000303|PubMed:27302341};
GN OrderedLocusNames=At1g64150 {ECO:0000312|Araport:AT1G64150};
GN ORFNames=F22C12.9 {ECO:0000312|EMBL:AAF24562.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=27302341; DOI=10.1016/j.molp.2016.05.015;
RA Wang C., Xu W., Jin H., Zhang T., Lai J., Zhou X., Zhang S., Liu S.,
RA Duan X., Wang H., Peng C., Yang C.;
RT "A putative chloroplast-localized Ca(2+)/H(+) antiporter CCHA1 is involved
RT in calcium and pH homeostasis and required for PSII function in
RT Arabidopsis.";
RL Mol. Plant 9:1183-1196(2016).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, SUBUNIT, AND
RP TOPOLOGY.
RX PubMed=27020959; DOI=10.1105/tpc.15.00812;
RA Schneider A., Steinberger I., Herdean A., Gandini C., Eisenhut M., Kurz S.,
RA Morper A., Hoecker N., Ruehle T., Labs M., Fluegge U.I., Geimer S.,
RA Schmidt S.B., Husted S., Weber A.P., Spetea C., Leister D.;
RT "The evolutionarily conserved protein PHOTOSYNTHESIS AFFECTED MUTANT71 is
RT required for efficient manganese uptake at the thylakoid membrane in
RT Arabidopsis.";
RL Plant Cell 28:892-910(2016).
CC -!- FUNCTION: Mn(2+)/H(+) exchanger, which transport Mn(2+)from the
CC chloroplast stroma into the acidic thylakoid lumen (PubMed:27020959).
CC Might be a chloroplast-localized Ca(2+)/H(+) antiporter
CC (PubMed:27302341). Regulates Ca(2+), Mn(2+) and pH homeostasis
CC (PubMed:27302341). Required for chloroplast development
CC (PubMed:27302341). {ECO:0000269|PubMed:27020959,
CC ECO:0000269|PubMed:27302341}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:27020959}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:27302341}; Multi-pass membrane protein
CC {ECO:0000255}. Thylakoid {ECO:0000269|PubMed:27020959}.
CC -!- DISRUPTION PHENOTYPE: Defects in photosynthesis and reduced growth rate
CC (PubMed:27302341, PubMed:27020959). Pale yellow leaves with reduced
CC PSII activity (PubMed:27302341). Altered Ca(2+) and Mn(2+) partitioning
CC in chloroplasts and reduced Mn(2+) binding to PSII (PubMed:27020959).
CC {ECO:0000269|PubMed:27020959, ECO:0000269|PubMed:27302341}.
CC -!- SIMILARITY: Belongs to the GDT1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF24562.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007764; AAF24562.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34202.1; -; Genomic_DNA.
DR EMBL; AY045639; AAK73997.1; -; mRNA.
DR EMBL; AY143875; AAN28814.1; -; mRNA.
DR RefSeq; NP_564825.1; NM_105088.4.
DR AlphaFoldDB; Q94AX5; -.
DR BioGRID; 27940; 25.
DR IntAct; Q94AX5; 25.
DR STRING; 3702.AT1G64150.1; -.
DR TCDB; 2.A.106.1.5; the ca(2+):h(+) antiporter-2 (caca2) family.
DR PaxDb; Q94AX5; -.
DR PRIDE; Q94AX5; -.
DR ProteomicsDB; 248747; -.
DR EnsemblPlants; AT1G64150.1; AT1G64150.1; AT1G64150.
DR GeneID; 842719; -.
DR Gramene; AT1G64150.1; AT1G64150.1; AT1G64150.
DR KEGG; ath:AT1G64150; -.
DR Araport; AT1G64150; -.
DR TAIR; locus:2024628; AT1G64150.
DR eggNOG; KOG2881; Eukaryota.
DR HOGENOM; CLU_050130_1_0_1; -.
DR InParanoid; Q94AX5; -.
DR OMA; VRCISSK; -.
DR OrthoDB; 919566at2759; -.
DR PhylomeDB; Q94AX5; -.
DR PRO; PR:Q94AX5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q94AX5; baseline and differential.
DR Genevisible; Q94AX5; AT.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009534; C:chloroplast thylakoid; IDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015085; F:calcium ion transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IMP:TAIR.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IMP:TAIR.
DR GO; GO:0046873; F:metal ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IDA:TAIR.
DR GO; GO:0006816; P:calcium ion transport; IMP:TAIR.
DR GO; GO:0019722; P:calcium-mediated signaling; IEP:TAIR.
DR GO; GO:0032468; P:Golgi calcium ion homeostasis; IBA:GO_Central.
DR GO; GO:0032472; P:Golgi calcium ion transport; IBA:GO_Central.
DR GO; GO:0071421; P:manganese ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0010270; P:photosystem II oxygen evolving complex assembly; IMP:TAIR.
DR InterPro; IPR001727; Gdt1.
DR PANTHER; PTHR12608; PTHR12608; 1.
DR Pfam; PF01169; UPF0016; 2.
DR PROSITE; PS01214; UPF0016; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Plastid; Reference proteome; Thylakoid;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..73
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 74..370
FT /note="Protein PAM71, chloroplastic"
FT /id="PRO_0000398764"
FT TOPO_DOM 74..113
FT /note="Stromal"
FT /evidence="ECO:0000305"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 135..161
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000305"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 183..188
FT /note="Stromal"
FT /evidence="ECO:0000305"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 210..228
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000305"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 250..275
FT /note="Stromal"
FT /evidence="ECO:0000305"
FT TRANSMEM 276..296
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 297..315
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000305"
FT TRANSMEM 316..336
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 337..348
FT /note="Stromal"
FT /evidence="ECO:0000305"
FT TRANSMEM 349..369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 370
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000269|PubMed:27020959"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..38
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 54
FT /note="Y -> S (in Ref. 3; AAN28814/AAK73997)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 370 AA; 39074 MW; 5D3FF0E824889BE7 CRC64;
MLSLNLSESL RIPFQNPRPP KSDFSSTSSS PSSSSRRCVS AYPIPIGFSV RNQYFSRCLT
QLRRNESQQL GFRCFQRNDA ACYLEKAESE EHDRNLDVLV ESSIAHSRRE IQRVLMFLAV
SGSVALLGTD PAFAASSIPN VTQSLVTSFG DLGDISSGFA SAFLLIFFSE LGDKTFFIAA
LLAARNSAAT VFVGTFGALG IMTIISVVLG RTFHYVDEVL PFRFGGTDLP IDDIAAVCLL
VYFGVSTLLD AVSDEGKADE EQKEAELAVS ELSGNGAGIV AAANTIISTF ALVFVAEWGD
KSFFSTIALA AASSPLGVIA GALAGHGAAT LLAVLGGSLL GNFLSEKAIA YVGGVLFLVF
AAVTVAEIVT
