ID   A32_VACCW               Reviewed;         270 AA.
AC   P68615; P21055; Q80HU4; Q89195;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=Protein A32;
GN   OrderedLocusNames=VACWR155; ORFNames=A32L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1856205; DOI=10.1016/s0021-9258(18)92757-2;
RA   Amegadzie B.Y., Ahn B.-Y., Moss B.;
RT   "Identification, sequence, and expression of the gene encoding a Mr 35,000
RT   subunit of the vaccinia virus DNA-dependent RNA polymerase.";
RL   J. Biol. Chem. 266:13712-13718(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2045793; DOI=10.1099/0022-1317-72-6-1349;
RA   Smith G.L., Chan Y.S., Howard S.T.;
RT   "Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the
RT   right inverted terminal repeat.";
RL   J. Gen. Virol. 72:1349-1376(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   POSSIBLE FUNCTION.
RX   PubMed=8470370; DOI=10.1007/bf01702351;
RA   Koonin E.V., Senkevich T.G., Chernos V.I.;
RT   "Gene A32 product of vaccinia virus may be an ATPase involved in viral DNA
RT   packaging as indicated by sequence comparisons with other putative viral
RT   ATPases.";
RL   Virus Genes 7:89-94(1993).
RN   [5]
RP   FUNCTION.
RX   PubMed=9621036; DOI=10.1128/jvi.72.7.5769-5780.1998;
RA   Cassetti M.C., Merchlinsky M., Wolffe E.J., Weisberg A.S., Moss B.;
RT   "DNA packaging mutant: repression of the vaccinia virus A32 gene results in
RT   noninfectious, DNA-deficient, spherical, enveloped particles.";
RL   J. Virol. 72:5769-5780(1998).
RN   [6]
RP   INTERACTION WITH A11.
RX   PubMed=15890898; DOI=10.1128/jvi.79.11.6598-6609.2005;
RA   Resch W., Weisberg A.S., Moss B.;
RT   "Vaccinia virus nonstructural protein encoded by the A11R gene is required
RT   for formation of the virion membrane.";
RL   J. Virol. 79:6598-6609(2005).
CC   -!- FUNCTION: Participates in viral DNA packaging and virion morphogenesis.
CC       {ECO:0000269|PubMed:9621036}.
CC   -!- SUBUNIT: Interacts with protein A11. {ECO:0000269|PubMed:15890898}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae A32 protein family.
CC       {ECO:0000305}.
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DR   EMBL; M61187; AAA48329.1; -; Genomic_DNA.
DR   EMBL; D11079; BAA01804.1; -; Genomic_DNA.
DR   EMBL; X57318; CAA40582.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89434.1; -; Genomic_DNA.
DR   PIR; JQ1768; JQ1768.
DR   RefSeq; YP_233037.1; NC_006998.1.
DR   IntAct; P68615; 2.
DR   MINT; P68615; -.
DR   DNASU; 3707685; -.
DR   GeneID; 3707685; -.
DR   KEGG; vg:3707685; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR006758; A32L.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF04665; Pox_A32; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..270
FT                   /note="Protein A32"
FT                   /id="PRO_0000099314"
FT   BINDING         55..62
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   270 AA;  30904 MW;  CA9EDA944EBA57EA CRC64;
     MNCFQEKQFS RENLLKMPFR MVLTGGSGSG KTIYLLSLFS TLVKKYKHIF LFTPVYNPDY
     DGYIWPNHIN FVSSQESLEY NLIRTKSNIE KCIAVAQNHK KSAHFLLIFD DVGDKLSKCN
     TLIEFLNFGR HLNTSIILLC QTYRHVPILG RANITHFCSF NISISDAENM LRSMPVKGKR
     KDILNMLNMI QTARSNNRLA IIIEDSVFCE GELRICTDTA DKDVIEQKLN IDILVNQYSH
     MKKNLNAILE SKKTKLCNSD QSSSSKNVSS