ID   PANB_SCHPO              Reviewed;         267 AA.
AC   Q09672;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=Probable 3-methyl-2-oxobutanoate hydroxymethyltransferase;
DE            EC=2.1.2.11;
DE   AltName: Full=Ketopantoate hydroxymethyltransferase;
GN   ORFNames=SPAC5H10.09c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-
CC         oxobutanoate + H2O = (6S)-5,6,7,8-tetrahydrofolate + 2-
CC         dehydropantoate; Xref=Rhea:RHEA:11824, ChEBI:CHEBI:11561,
CC         ChEBI:CHEBI:11851, ChEBI:CHEBI:15377, ChEBI:CHEBI:15636,
CC         ChEBI:CHEBI:57453; EC=2.1.2.11;
CC   -!- PATHWAY: Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-
CC       pantoate from 3-methyl-2-oxobutanoate: step 1/2.
CC   -!- SIMILARITY: Belongs to the PanB family. {ECO:0000305}.
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DR   EMBL; CU329670; CAA89959.1; -; Genomic_DNA.
DR   PIR; S55487; S55487.
DR   RefSeq; NP_592822.1; NM_001018222.2.
DR   AlphaFoldDB; Q09672; -.
DR   SMR; Q09672; -.
DR   BioGRID; 279181; 19.
DR   STRING; 4896.SPAC5H10.09c.1; -.
DR   MaxQB; Q09672; -.
DR   PaxDb; Q09672; -.
DR   EnsemblFungi; SPAC5H10.09c.1; SPAC5H10.09c.1:pep; SPAC5H10.09c.
DR   GeneID; 2542731; -.
DR   KEGG; spo:SPAC5H10.09c; -.
DR   PomBase; SPAC5H10.09c; -.
DR   VEuPathDB; FungiDB:SPAC5H10.09c; -.
DR   eggNOG; KOG2949; Eukaryota.
DR   HOGENOM; CLU_036645_1_0_1; -.
DR   InParanoid; Q09672; -.
DR   OMA; VLVWTDM; -.
DR   PhylomeDB; Q09672; -.
DR   UniPathway; UPA00028; UER00003.
DR   PRO; PR:Q09672; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005739; C:mitochondrion; HDA:PomBase.
DR   GO; GO:0003864; F:3-methyl-2-oxobutanoate hydroxymethyltransferase activity; ISS:PomBase.
DR   GO; GO:0000287; F:magnesium ion binding; IBA:GO_Central.
DR   GO; GO:0015940; P:pantothenate biosynthetic process; ISS:PomBase.
DR   CDD; cd06557; KPHMT-like; 1.
DR   Gene3D; 3.20.20.60; -; 1.
DR   HAMAP; MF_00156; PanB; 1.
DR   InterPro; IPR003700; Pantoate_hydroxy_MeTrfase.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR   PANTHER; PTHR20881; PTHR20881; 1.
DR   Pfam; PF02548; Pantoate_transf; 1.
DR   PIRSF; PIRSF000388; Pantoate_hydroxy_MeTrfase; 1.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   TIGRFAMs; TIGR00222; panB; 1.
PE   3: Inferred from homology;
KW   Pantothenate biosynthesis; Reference proteome; Transferase.
FT   CHAIN           1..267
FT                   /note="Probable 3-methyl-2-oxobutanoate
FT                   hydroxymethyltransferase"
FT                   /id="PRO_0000184926"
SQ   SEQUENCE   267 AA;  29111 MW;  B0B4FE50752960E9 CRC64;
     MSLKQITIST LRQWKLANKK FACITAYDAS FSRLFAEQGM PVMLVGDSLG MTAQGHSTTL
     PVSVEDIAYH TKSVRRGAPN RLLMADLPFM SYSTWEDACK NAATVMRAGA NIVKIEGGGN
     WIFEIVQRLT ERSVPVAGHL GLTPQSVNIF GGYKIQGREQ SAAARLIENA QQLEKFGAQL
     LVLECIPESL AEQITKTISI PTIGIGAGKH TDGQILVMHD ALGITGGRPP KFAKNFLSGA
     GDIRTAIQRY IYEVEQGLYP AEEHSFQ