ID   A33_VACCC               Reviewed;         185 AA.
AC   P68616; P21056;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   29-SEP-2021, entry version 45.
DE   RecName: Full=Protein A33;
GN   ORFNames=A33R;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
CC   -!- FUNCTION: Coordinates the incorporation of A36 into intracellular
CC       enveloped virion (IEV) membranes and, subsequently, the production of
CC       actin tails. Therefore plays an essential role in efficient cell-to-
CC       cell spread of viral particles (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer; disulfide-linked. Interacts with B5. Interacts (via
CC       C-terminus) with protein A36 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass type
CC       II membrane protein {ECO:0000305}. Host membrane {ECO:0000305}; Single-
CC       pass type II membrane protein {ECO:0000305}. Note=Component of the
CC       enveloped virion (EV) membrane.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae A33 protein family.
CC       {ECO:0000305}.
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DR   EMBL; M35027; AAA48160.1; -; Genomic_DNA.
DR   PIR; H42520; H42520.
DR   SMR; P68616; -.
DR   Proteomes; UP000008269; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.10.100.10; -; 1.
DR   InterPro; IPR016186; C-type_lectin-like/link_sf.
DR   InterPro; IPR009238; Chordopox_A33R.
DR   Pfam; PF05966; Chordopox_A33R; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Glycoprotein; Host membrane; Membrane; Reference proteome;
KW   Signal-anchor; Transmembrane; Transmembrane helix; Viral envelope protein;
KW   Virion.
FT   CHAIN           1..185
FT                   /note="Protein A33"
FT                   /id="PRO_0000099316"
FT   TOPO_DOM        1..33
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        34..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        57..185
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000250"
FT   REGION          98..185
FT                   /note="C-type lectin-like domain"
FT   CARBOHYD        125
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        135
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
FT   DISULFID        62
FT                   /note="Interchain"
SQ   SEQUENCE   185 AA;  20506 MW;  201CF65C2D2076A4 CRC64;
     MMTPENDEEQ TSVFSATVYG DKIQGKNKRK RVIGLCIRIS MVISLLSMIT MSAFLIVRLN
     QCMSANEAAI TDAAVAVAAA SSTHRKVASS TTQYDHKESC NGLYYQGSCY ILHSDYQLFS
     DAKANCTAES STLPNKSDVL ITWLIDYVED TWGSDGNPIT KTTSDYQDSD VSQEVRKYFC
     VKTMN