ID   A33_VAR67               Reviewed;         184 AA.
AC   P0DON1; P33850;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   12-AUG-2020, entry version 5.
DE   RecName: Full=Protein A33;
GN   ORFNames=A33R, A36R;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Coordinates the incorporation of A36 into intracellular
CC       enveloped virion (IEV) membranes and, subsequently, the production of
CC       actin tails. Therefore plays an essential role in efficient cell-to-
CC       cell spread of viral particles (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer; disulfide-linked. Interacts with B5. Interacts (via
CC       C-terminus) with protein A36 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass type
CC       II membrane protein {ECO:0000305}. Host membrane {ECO:0000305}; Single-
CC       pass type II membrane protein {ECO:0000305}. Note=Component of the
CC       enveloped virion (EV) membrane.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae A33 protein family.
CC       {ECO:0000305}.
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DR   EMBL; X69198; CAA49081.1; -; Genomic_DNA.
DR   EMBL; X67115; CAA47507.1; -; Genomic_DNA.
DR   PIR; A36852; A36852.
DR   RefSeq; NP_042184.1; NC_001611.1.
DR   SMR; P0DON1; -.
DR   GeneID; 1486514; -.
DR   KEGG; vg:1486514; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.10.100.10; -; 1.
DR   InterPro; IPR016186; C-type_lectin-like/link_sf.
DR   InterPro; IPR009238; Chordopox_A33R.
DR   InterPro; IPR016187; CTDL_fold.
DR   Pfam; PF05966; Chordopox_A33R; 1.
DR   SUPFAM; SSF56436; SSF56436; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Glycoprotein; Host membrane; Membrane; Reference proteome;
KW   Signal-anchor; Transmembrane; Transmembrane helix; Viral envelope protein;
KW   Virion.
FT   CHAIN           1..184
FT                   /note="Protein A33"
FT                   /id="PRO_0000099318"
FT   TOPO_DOM        1..33
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        34..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        57..184
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000250"
FT   REGION          98..184
FT                   /note="C-type lectin-like domain"
FT   CARBOHYD        134
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
FT   DISULFID        62
FT                   /note="Interchain"
SQ   SEQUENCE   184 AA;  20512 MW;  8729A4A636DBB871 CRC64;
     MMTPENDEEQ TSVFSATVYG DKIQGKNKRK RVIGICIRIS MVISLLSMIT MSAFLIVRLN
     QCMSANEAAI TDATAVAAAL STHRKVASST TQYKHQESCN GLYYQGSCYI FHSDYQLFSD
     AKANCATESS TLPNKSDVLT TWLIDYVEDT WGSDGNPITK TTTDYQDSDV SQEVRKYFCV
     KTMN