ID   A33_VARV                Reviewed;         184 AA.
AC   P0DON2; P33850;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   29-SEP-2021, entry version 10.
DE   RecName: Full=Protein A33;
GN   ORFNames=A33R, A36R;
OS   Variola virus.
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=10255;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Harvey;
RX   PubMed=1331292; DOI=10.1099/0022-1317-73-11-2887;
RA   Aguado B., Selmes I.P., Smith G.L.;
RT   "Nucleotide sequence of 21.8 kbp of variola major virus strain Harvey and
RT   comparison with vaccinia virus.";
RL   J. Gen. Virol. 73:2887-2902(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Bangladesh-1975;
RX   PubMed=8264798; DOI=10.1038/366748a0;
RA   Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C.,
RA   Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A.,
RA   Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.;
RT   "Potential virulence determinants in terminal regions of variola smallpox
RT   virus genome.";
RL   Nature 366:748-751(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Garcia-1966;
RA   Shchelkunov S.N., Totmenin A.V., Resenchuk S.M., Blinov V.M.,
RA   Sandakhchiev L.S.;
RL   Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Coordinates the incorporation of A36 into intracellular
CC       enveloped virion (IEV) membranes and, subsequently, the production of
CC       actin tails. Therefore plays an essential role in efficient cell-to-
CC       cell spread of viral particles (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer; disulfide-linked. Interacts with B5. Interacts (via
CC       C-terminus) with protein A36 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass type
CC       II membrane protein {ECO:0000305}. Host membrane {ECO:0000305}; Single-
CC       pass type II membrane protein {ECO:0000305}. Note=Component of the
CC       enveloped virion (EV) membrane.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae A33 protein family.
CC       {ECO:0000305}.
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DR   EMBL; L22579; AAA60888.1; -; Genomic_DNA.
DR   EMBL; X76266; CAA53862.1; -; Genomic_DNA.
DR   PIR; B72168; B72168.
DR   PIR; T28578; T28578.
DR   RefSeq; NP_042184.1; NC_001611.1.
DR   SMR; P0DON2; -.
DR   GeneID; 1486514; -.
DR   KEGG; vg:1486514; -.
DR   Proteomes; UP000119805; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.10.100.10; -; 1.
DR   InterPro; IPR016186; C-type_lectin-like/link_sf.
DR   InterPro; IPR009238; Chordopox_A33R.
DR   InterPro; IPR016187; CTDL_fold.
DR   Pfam; PF05966; Chordopox_A33R; 1.
DR   SUPFAM; SSF56436; SSF56436; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Glycoprotein; Host membrane; Membrane; Signal-anchor;
KW   Transmembrane; Transmembrane helix; Viral envelope protein; Virion.
FT   CHAIN           1..184
FT                   /note="Protein A33"
FT                   /id="PRO_0000448164"
FT   TOPO_DOM        1..33
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        34..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        57..184
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000250"
FT   REGION          98..184
FT                   /note="C-type lectin-like domain"
FT   CARBOHYD        134
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
FT   DISULFID        62
FT                   /note="Interchain"
SQ   SEQUENCE   184 AA;  20512 MW;  8729A4A636DBB871 CRC64;
     MMTPENDEEQ TSVFSATVYG DKIQGKNKRK RVIGICIRIS MVISLLSMIT MSAFLIVRLN
     QCMSANEAAI TDATAVAAAL STHRKVASST TQYKHQESCN GLYYQGSCYI FHSDYQLFSD
     AKANCATESS TLPNKSDVLT TWLIDYVEDT WGSDGNPITK TTTDYQDSDV SQEVRKYFCV
     KTMN