ID   A34_VACCW               Reviewed;         168 AA.
AC   P24761; Q76ZP2;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Protein A34;
GN   OrderedLocusNames=VACWR157; ORFNames=A34R, SALL4R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2045793; DOI=10.1099/0022-1317-72-6-1349;
RA   Smith G.L., Chan Y.S., Howard S.T.;
RT   "Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the
RT   right inverted terminal repeat.";
RL   J. Gen. Virol. 72:1349-1376(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1856205; DOI=10.1016/s0021-9258(18)92757-2;
RA   Amegadzie B.Y., Ahn B.-Y., Moss B.;
RT   "Identification, sequence, and expression of the gene encoding a Mr 35,000
RT   subunit of the vaccinia virus DNA-dependent RNA polymerase.";
RL   J. Biol. Chem. 266:13712-13718(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=1738204; DOI=10.1128/jvi.66.3.1610-1621.1992;
RA   Duncan S.A., Smith G.L.;
RT   "Identification and characterization of an extracellular envelope
RT   glycoprotein affecting vaccinia virus egress.";
RL   J. Virol. 66:1610-1621(1992).
RN   [5]
RP   INTERACTION WITH PROTEINS A36 AND B5.
RX   PubMed=10074134; DOI=10.1128/jvi.73.4.2863-2875.1999;
RA   Rottger S., Frischknecht F., Reckmann I., Smith G.L., Way M.;
RT   "Interactions between vaccinia virus IEV membrane proteins and their roles
RT   in IEV assembly and actin tail formation.";
RL   J. Virol. 73:2863-2875(1999).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=16509968; DOI=10.1186/1743-422x-3-10;
RA   Yoder J.D., Chen T.S., Gagnier C.R., Vemulapalli S., Maier C.S.,
RA   Hruby D.E.;
RT   "Pox proteomics: mass spectrometry analysis and identification of Vaccinia
RT   virion proteins.";
RL   Virol. J. 3:10-10(2006).
RN   [7]
RP   FUNCTION.
RX   PubMed=18094186; DOI=10.1128/jvi.01969-07;
RA   Perdiguero B., Lorenzo M.M., Blasco R.;
RT   "Vaccinia virus A34 glycoprotein determines the protein composition of the
RT   extracellular virus envelope.";
RL   J. Virol. 82:2150-2160(2008).
CC   -!- FUNCTION: Required for the envelopment of intracellular viral particles
CC       and egress of enveloped virions from the infected cell.
CC       {ECO:0000269|PubMed:18094186}.
CC   -!- SUBUNIT: Interacts with proteins A36 AND B5.
CC       {ECO:0000269|PubMed:10074134}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass type
CC       II membrane protein {ECO:0000305}. Note=Present in the enveloped virion
CC       (EV) membrane. {ECO:0000269|PubMed:1738204}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae A34 protein family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D11079; BAA01806.1; -; Genomic_DNA.
DR   EMBL; M61187; AAA48331.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89436.1; -; Genomic_DNA.
DR   PIR; JQ1770; JQ1770.
DR   RefSeq; YP_233039.1; NC_006998.1.
DR   SMR; P24761; -.
DR   IntAct; P24761; 4.
DR   MINT; P24761; -.
DR   DNASU; 3707687; -.
DR   GeneID; 3707687; -.
DR   KEGG; vg:3707687; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.10.100.10; -; 1.
DR   InterPro; IPR001304; C-type_lectin-like.
DR   InterPro; IPR016186; C-type_lectin-like/link_sf.
DR   InterPro; IPR016187; CTDL_fold.
DR   Pfam; PF00059; Lectin_C; 1.
DR   SUPFAM; SSF56436; SSF56436; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Glycoprotein; Membrane; Reference proteome; Signal-anchor;
KW   Transmembrane; Transmembrane helix; Virion.
FT   CHAIN           1..168
FT                   /note="Protein A34"
FT                   /id="PRO_0000099320"
FT   TOPO_DOM        1..14
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        15..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        38..168
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          54..163
FT                   /note="C-type lectin"
FT   CARBOHYD        133
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
FT   DISULFID        75..162
FT                   /evidence="ECO:0000250"
FT   DISULFID        141..154
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   168 AA;  19555 MW;  69F72EA9D971F19F CRC64;
     MKSLNRQTVS RFKKLSVPAA IMMILSTIIS GIGTFLHYKE ELMPSACANG WIQYDKHCYL
     DTNIKMSTDN AVYQCRKLRA RLPRPDTRHL RVLFSIFYKD YWVSLKKTND KWLDINNDKD
     IDISKLTNFK QLNSTTDAEA CYIYKSGKLV KTVCKSTQSV LCVKKFYK