ID   A35_VACCW               Reviewed;         176 AA.
AC   Q01232; Q76ZP1;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Protein A35;
DE   Flags: Precursor;
GN   OrderedLocusNames=VACWR158; ORFNames=A35R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2045793; DOI=10.1099/0022-1317-72-6-1349;
RA   Smith G.L., Chan Y.S., Howard S.T.;
RT   "Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the
RT   right inverted terminal repeat.";
RL   J. Gen. Virol. 72:1349-1376(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1856205; DOI=10.1016/s0021-9258(18)92757-2;
RA   Amegadzie B.Y., Ahn B.-Y., Moss B.;
RT   "Identification, sequence, and expression of the gene encoding a Mr 35,000
RT   subunit of the vaccinia virus DNA-dependent RNA polymerase.";
RL   J. Biol. Chem. 266:13712-13718(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   INDUCTION.
RX   PubMed=16352555; DOI=10.1128/jvi.80.1.306-313.2006;
RA   Roper R.L.;
RT   "Characterization of the vaccinia virus A35R protein and its role in
RT   virulence.";
RL   J. Virol. 80:306-313(2006).
RN   [5]
RP   FUNCTION.
RX   PubMed=19828608; DOI=10.1128/jvi.01802-09;
RA   Rehm K.E., Jones G.J., Tripp A.A., Metcalf M.W., Roper R.L.;
RT   "The poxvirus A35 protein is an immunoregulator.";
RL   J. Virol. 84:418-425(2010).
RN   [6]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=19954808; DOI=10.1016/j.virol.2009.11.008;
RA   Rehm K.E., Connor R.F., Jones G.J., Yimbu K., Roper R.L.;
RT   "Vaccinia virus A35R inhibits MHC class II antigen presentation.";
RL   Virology 397:176-186(2010).
CC   -!- FUNCTION: Mildly affects the expression of MHC class II molecules on
CC       the surface of host antigen presenting cells (APCs).
CC       {ECO:0000269|PubMed:19828608, ECO:0000269|PubMed:19954808}.
CC   -!- SUBCELLULAR LOCATION: Host endosome {ECO:0000269|PubMed:19954808}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:16352555}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae A35 protein family.
CC       {ECO:0000305}.
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DR   EMBL; D11079; BAA01807.1; -; Genomic_DNA.
DR   EMBL; X57318; CAA40584.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89437.1; -; Genomic_DNA.
DR   PIR; S29918; S29918.
DR   RefSeq; YP_233040.1; NC_006998.1.
DR   DNASU; 3707688; -.
DR   GeneID; 3707688; -.
DR   KEGG; vg:3707688; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0044174; C:host cell endosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0039505; P:suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II; IEA:UniProtKB-KW.
DR   InterPro; IPR009247; Chordopox_A35R.
DR   Pfam; PF05989; Chordopox_A35R; 1.
PE   2: Evidence at transcript level;
KW   Host endosome; Host-virus interaction;
KW   Inhibition of host adaptive immune response by virus;
KW   Inhibition of host MHC class II molecule presentation by virus;
KW   Reference proteome; Signal; Viral immunoevasion.
FT   SIGNAL          1..14
FT                   /evidence="ECO:0000255"
FT   CHAIN           15..176
FT                   /note="Protein A35"
FT                   /id="PRO_0000040598"
SQ   SEQUENCE   176 AA;  20031 MW;  D99E9E46CE171078 CRC64;
     MDAAFVITPM GVLTITDTLY DDLDISIMDF IGPYIIGNIK TVQIDVRDIK YSDMQKCYFS
     YKGKIVPQDS NDLARFNIYS ICAAYRSKNT IIIACDYDIM LDIEDKHQPF YLFPSIDVFN
     ATIIEAYNLY TAGDYHLIIN PSDNLKMKLL FNSSFCISDG NGWIIIDGKC NSNFLS